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学科主题: 临床医学
题名:
Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis
作者: Ding, CH; Li, Q; Xiong, ZY; Zhou, AW; Jones, G; Xu, SY
关键词: type II collagen ; oral tolerance ; adjuvant arthritis ; synoviocyte ; TGF-beta
刊名: CLINICAL AND EXPERIMENTAL IMMUNOLOGY
发表日期: 2003-06-01
卷: 132, 期:3, 页:416-423
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Immunology
研究领域[WOS]: Immunology
关键词[WOS]: MYELIN BASIC-PROTEIN ; GROWTH-FACTOR-BETA ; RHEUMATOID-ARTHRITIS ; AUTOIMMUNE-DISEASES ; TOLERANCE INDUCTION ; DOWN-REGULATION ; PEYERS-PATCHES ; DOUBLE-BLIND ; LEWIS RATS ; T-CELLS
英文摘要:

The objective of this study was to investigate the effect of the oral administration of type II collagen (CII) on pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis (AA). Sprague-Dawley rats were fed with bovine CII either before immunization with Complete Freund′s adjuvant (CFA) or after initiation of arthritis. Hind paw secondary swelling was measured and synoviocytes were harvested. Sera from portal vein of oral tolerized rats were collected and in vitro synoviocytes culture or synoviocytes-Peyer′s Patches (PP) cells coculture system were developed. Interleukin (IL)-1 activity was measured by a mouse thymocyte activation assayed by MTT dye reduction and tumour necrosis factor (TNF) activity was measured by an L929 cytotoxicity bioassay. Nitric oxide (NO) and malondialdehyde (MDA) levels were measured by biochemical methods. We found that feeding with CII (5, 50 and 500 mug/kg) for 7 days before immunization significantly suppressed hind paw secondary swelling measured at day 16,20,24 and 28 (all, P < 0.01) and pro-inflammatory mediator (IL-1, TNF, NO and MDA) production by synoviocytes (all P < 0.01) in rats with AA. Feeding with CII (5, 50 and 500 mug/kg) for 7 days after initiation of arthritis had a similar effect. CII (1, 10, 100 mug/ml) had no effect on IL-1 and TNF production by synoviocytes in vitro, but CH 10 mug/ml suppressed IL-1 and TNF production by synoviocytes-PP cells coculture system (P < 0.01), which was antagonized by anti-TGF-β antibody (10 μg/ml) (P < 0.01). Portal serum (1 : 10) from oral tolerized rats suppressed IL-1 and TNF production by synoviocytes (P < 0.01), which was also antagonized by anti-TGF-β antibody (10 μg/ml) (P < 0.01). We conclude that oral administration of CII had prophylactic and therapeutic effects on AA and over-production of IL-1,TNF, NO and MDA by synoviocytes was suppressed. Bystander active suppression may be the main mechanism of oral CH in the suppression of synoviocyte function.

语种: 英语
WOS记录号: WOS:000183693900006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67499
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Anhui Med Univ, Inst Clin Pharmacol, Hefei, Peoples R China
2.Univ Tasmania, Discipline Med, Hobart, Tas, Australia
3.Peking Univ, Shenzhen Hosp, Shenzhen, Peoples R China
4.Univ Tasmania, Menzies Ctr Populat Hlth Res, Hobart, Tas 7000, Australia

Recommended Citation:
Ding, CH,Li, Q,Xiong, ZY,et al. Oral administration of type II collagen suppresses pro-inflammatory mediator production by synoviocytes in rats with adjuvant arthritis[J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY,2003,132(3):416-423.
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