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Interactions Between N-Terminal Extracellular Tail of CCR4 and Natural Products of Licorice Using Capillary Electrophoresis
Li, Meina1,2; Wang, Qing1,3; Ling, Xiaomei1,2; Yang, Lu1,2; Li, Zhongjie1,2; Ye, Min1,3; Wang, Ying4
关键词Capillary electrophoresis CCR4 antagonists Licorice Interactions
刊名CHROMATOGRAPHIA
2013-07-01
DOI10.1007/s10337-013-2474-y
76期:13-14页:811-819
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
资助者National Natural Science Foundation ; Natural Science Foundation of Beijing ; Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation ; Natural Science Foundation of Beijing ; Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]IN-VITRO ; FLAVONOIDS ; ANTAGONISTS ; INHIBITORS ; BINDING ; LIGAND ; MICE
英文摘要

Licorice is gaining popularity because of its significant biological anti-inflammatory activity, while CC chemokine receptor 4 (CCR4) has been identified as a potentially important drug target for the treatment of inflammatory diseases. Capillary electrophoresis was developed for screening for the first time of CCR4 antagonists from natural products of licorice. The interactions between natural products of licorice and ML40, the equivalent peptide derived from the N-terminal of CCR4, were determined. Twenty-eight ingredients were isolated and the results showed that ten of them interacted with ML40 compared with the positive control S009. The binding constants of the compounds to ML40 were calculated and the binding constant of liquiritin apioside (LA) was the largest among them (3.636 +/- A 0.2185) x 10(4) M-1. The CCR4 antagonisms of the compounds that showed strong integration with ML40 were also confirmed by chemotaxis inhibition in which they displayed different degrees of inhibition to CCL22/CCL17-induced HEK293 chemotaxis. The methodology presented could be applied to automated high-throughput screening of potential antagonists of CCR4. This study provided the potential rationale for the development of anti-inflammatory compounds from natural products of licorice.

语种英语
所属项目编号30872292 ; 90813025 ; 81072612 ; 7102107 ; K2012004 ; 20110001110021
资助者National Natural Science Foundation ; Natural Science Foundation of Beijing ; Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China ; National Natural Science Foundation ; Natural Science Foundation of Beijing ; Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China
WOS记录号WOS:000320959000009
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67507
Collection北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100191, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100191, Peoples R China
4.Peking Univ, Sch Basic Med Sci, Dept Med Immunol, Ctr Human Dis Genom, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Li, Meina,Wang, Qing,Ling, Xiaomei,et al. Interactions Between N-Terminal Extracellular Tail of CCR4 and Natural Products of Licorice Using Capillary Electrophoresis[J]. CHROMATOGRAPHIA,2013,76(13-14):811-819.
APA Li, Meina.,Wang, Qing.,Ling, Xiaomei.,Yang, Lu.,Li, Zhongjie.,...&Wang, Ying.(2013).Interactions Between N-Terminal Extracellular Tail of CCR4 and Natural Products of Licorice Using Capillary Electrophoresis.CHROMATOGRAPHIA,76(13-14),811-819.
MLA Li, Meina,et al."Interactions Between N-Terminal Extracellular Tail of CCR4 and Natural Products of Licorice Using Capillary Electrophoresis".CHROMATOGRAPHIA 76.13-14(2013):811-819.
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