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学科主题: 基础医学
题名:
Hyperpolarization-activated, cyclic nucleotide-gated cation channels: Roles in the differential electrophysiological properties of rat primary afferent neurons
作者: Tu, HY; Deng, LB; Sun, Q; Yao, L; Han, JS; Wan, Y
关键词: hyperpolarization-activated ; cyclic nucleotide-gated channel ; I-h ; dorsal root ganglion ; ZD7288
刊名: JOURNAL OF NEUROSCIENCE RESEARCH
发表日期: 2004-06-01
DOI: 10.1002/jnr.20109
卷: 76, 期:5, 页:713-722
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: DORSAL-ROOT GANGLION ; CURRENT I-H ; PACEMAKER CHANNELS ; SYNAPTIC-TRANSMISSION ; SYMPATHETIC NEURONS ; SENSORY NEURONS ; HCN CHANNELS ; CURRENT I(H) ; SPINAL-CORD ; MODULATION
英文摘要:

The large, medium-sized, and small neurons of the dorsal root ganglion (DRG) have different functions in the processing of various senses. Hyperpolarization-activated, cyclic nucleotide-gated channels (HCN) contribute greatly to neuronal excitability. In the present study, which used whole-cell patch clamp techniques and immunohistochemical staining methods, the electrophysiological properties of DRG neurons were systematically compared, and the roles of HCN-1, -2, and -4 were examined. The main results were as follows. 1) The large neurons had significantly higher V-0.5 values (membrane potential at which the HCN channels were half-activated) and shorter time constants (T) than small or medium-sized DRG neurons. However, large DRG neurons had higher I-h density (HCN neuron current). 2) HCN-1 was found predominantly, but not exclusively, in large and medium-sized DRG neurons; HCN-2 was found in all DRG neurons; and HCN-4 was poorly visualized in all DRG neurons. HCN-1 and HCN-2 were colocalized in large and medium-sized neurons with immunostaining of adjacent sections. In the dorsal horn of the spinal cord, HCN-1, HCN-2, and HCN-4 were all expressed in laminae I-IV, although HCN-1 was not detectable in lamina II. 3) Blockade of 1, current in DRG neurons caused a significant decrease in V0.5, resting membrane potential, and repetitive firing number of action potential and a significant increase in time of rising phase of action potential. These results suggest that the different HCN channels in the three types of DRG neurons might contribute to their differential electrophysiological properties. (C) 2004 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000221772700013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67510
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: Peking Univ, Neurosci Res Inst, Key Lab Neurosci, Minist Educ, Beijing 100083, Peoples R China

Recommended Citation:
Tu, HY,Deng, LB,Sun, Q,et al. Hyperpolarization-activated, cyclic nucleotide-gated cation channels: Roles in the differential electrophysiological properties of rat primary afferent neurons[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2004,76(5):713-722.
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