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The saponin of red ginseng protects the cardiac myocytes against ischemic injury in vitro and in vivo
Li, Hai-Xia1,2; Han, Shu-Yan1; Ma, Xu1; Zhang, Ke1; Wang, Li1; Ma, Zhi-Zhong1; Tu, Peng-Fei1
关键词Red ginseng Saponins Myocardial ischemia Hemodynamics K-ATP Ca2+
刊名PHYTOMEDICINE
2012-04-15
DOI10.1016/j.phymed.2012.01.002
19期:6页:477-483
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]NEONATAL-RAT CARDIOMYOCYTES ; NITRIC-OXIDE ; MYOCARDIAL-ISCHEMIA ; AMERICAN GINSENG ; EXTRACT ; ISCHEMIA/REPERFUSION ; GINSENOSIDES ; INCREASES ; HYPOXIA
英文摘要

Steamed root of Panax ginseng C.A. Mayer, known as "red ginseng", differs from other ginseng preparations in terms of its saponin components and content, as some partly deglycosylated saponins are produced as artifacts during the steaming process. However, whether saponins derived from red ginseng (SRG) can have a protective effect on cardiomyocytes remains unknown. The present study aimed to explore the effect of SRG on myocardial ischemia in vitro and in vivo. MTT assays revealed that SRG pretreatment significantly increased the viability of cardiomyocytes injured by Na2S2O4 hypoxia in vitro. This effect was almost completely abolished by glibenclamide, a blocker of the ATP-sensitive potassium channel, but the cardioprotective activity of SRG was not influenced by the phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002.SRG also significantly reduced the Na2S2O4-induced increase in intracellular calcium, as shown by Fluo-3/AM probes with flow cytometry. Adult rat heart ischemia, which was induced by ligation of the left anterior descending coronary artery, was employed for the in vivo analysis. SRG pretreatment reduced infarct size and resulted in a higher left ventricle (LV) developed pressure, LV (+)dP/dt(max) and LV systolic pressure and lower LV (-)dP/dt(max) and LV end diastolic pressure after 24h of ischemia. Moreover, SRG significantly reduced the level of cardiac Troponin I(cTnI) in the serum, which suggests that cTnI, a protein component of the troponin regulatory complex involved in cardiac contractility, contributes to the SRG-mediated recovery of cardiac systolic function. In conclusion, this study is the first to provide evidence and a mechanistic analysis of the cardioprotective effects of SRG. SRG significantly attenuated myocardial ischemic injury by improving cardiac systole function, partly by reducing cTnI secretion and improving cardiac diastolic function. Also, SRG attenuated the Ca2+ overload in cardiomyocytes and modulated the K-ATP, but not PI3K, signaling pathway; taken together, these mechanisms synergistically reduced infarct size. (C) 2012 Elsevier GmbH. All rights reserved.

语种英语
WOS记录号WOS:000304576400001
项目编号30525043
资助机构National Science Fund for Distinguished Young Scholars of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67514
专题北京大学药学院
北京大学基础医学院
北京大学药学院_天然药物学系
医学人文研究院/公共教学部_哲学与社会科学系
北京大学第一临床医学院_检验科
北京大学第三临床医学院_耳鼻喉科
北京大学临床肿瘤学院_中西医结合科暨老年肿瘤科
作者单位1.Peking Univ, Hlth Sci Ctr, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
2.Zhengzhou Univ, Sch Pharmaceut Sci, Zhengzhou 450002, Peoples R China
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GB/T 7714
Li, Hai-Xia,Han, Shu-Yan,Ma, Xu,et al. The saponin of red ginseng protects the cardiac myocytes against ischemic injury in vitro and in vivo[J]. PHYTOMEDICINE,2012,19(6):477-483.
APA Li, Hai-Xia.,Han, Shu-Yan.,Ma, Xu.,Zhang, Ke.,Wang, Li.,...&Tu, Peng-Fei.(2012).The saponin of red ginseng protects the cardiac myocytes against ischemic injury in vitro and in vivo.PHYTOMEDICINE,19(6),477-483.
MLA Li, Hai-Xia,et al."The saponin of red ginseng protects the cardiac myocytes against ischemic injury in vitro and in vivo".PHYTOMEDICINE 19.6(2012):477-483.
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