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The transport pathways of polymer nanoparticles in MDCK epithelial cells
He, Bing; Jia, Zengrong; Du, Wenwen; Yu, Chao; Fan, Yuchen; Dai, Wenbing; Yuan, Lan; Zhang, Hua; Wang, Xueqing; Wang, Jiancheng; Zhang, Xuan; Zhang, Qiang
关键词Polymer nanoparticles Epithelial cells Transport mechanism Endocytosis Exocytosis Transcytosis
刊名BIOMATERIALS
2013-06-01
DOI10.1016/j.biomaterials.2013.01.100
34期:17页:4309-4326
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]PLGA NANOPARTICLES ; PHOSPHATIDYLINOSITOL 3-KINASE ; DEPENDENT PATHWAY ; GROWTH-FACTOR ; LIPID RAFTS ; IN-VITRO ; ENDOCYTOSIS ; MEMBRANE ; COMPARTMENTS ; EXOCYTOSIS
英文摘要

Epithelial cell membranes as the typical biological barrier constitute the prime obstacle for the transport of therapeutic agents including nanomedicines. The previous studies on the interaction between nanomedicines and cells are mostly emphasized on cellular uptake and intracellular trafficking, but seldom on epithelial cells, although more and more oral nanomedicines are available now. In an attempt to clarify the transport pathways of nanomedicines in epithelial cells, the different molecular mechanisms among endocytosis, exocytosis and transcytosis processes were carefully studied and compared here using a kind of polymer nanopartides (PNs) and MDCK epithelial cells as models. As the result, their similarity and difference were demonstrated. The similarities among all the three processes included the mediation of lipid rafts, the involvement of some protein kinases such as protein tyrosine kinase (PTK), protein kinase C (PKC) and phosphatidylinositol 3-kinase (P13K), and the existence of multiple pathways. However, the difference among these processes was very significant, including different pathways, and especially the disparate effects of lipid rafts and protein kinases for different processes. The endocytosis involved both lipid raft and clathrin mechanisms but no macropinocytosis, via the invagination of membrane but no pore formation, the exocytosis contained ER/Golgi and Golgi/PM pathways, and transcytosis included AEE/CE/BSE and Golgi/BSE pathways. The roles of lipid rafts on endocytosis were positive but that on exocytosis and transcytosis was negative. The impacts of PTK and PKC on endocytosis were positive, while the influences of PTK, PKC and P13K on AEE/CE/BSE, as well as PTK and P13K on Golgi/BSE transcytosis pathways were negative. Moreover, the discrepancy between inward and outward transport of PNs elucidated an interesting fact that the endocytosis was rather easy and outward transport including exocytosis and transcytosis was rather difficult. Finally, it was indicated by comparison with previous reports that the molecular mechanisms between PNs and macromolecules such as proteins were also dissimilar. (C) 2013 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000317700400013
项目编号2009CB930300 ; 81130059
资助机构National Basic Research Program of China ; National Natural Science Foundation of China
引用统计
被引频次:59[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67516
专题北京大学药学院
北京大学医学信息学中心
北京大学基础医学院
北京大学药学院_药剂学系
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
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GB/T 7714
He, Bing,Jia, Zengrong,Du, Wenwen,et al. The transport pathways of polymer nanoparticles in MDCK epithelial cells[J]. BIOMATERIALS,2013,34(17):4309-4326.
APA He, Bing.,Jia, Zengrong.,Du, Wenwen.,Yu, Chao.,Fan, Yuchen.,...&Zhang, Qiang.(2013).The transport pathways of polymer nanoparticles in MDCK epithelial cells.BIOMATERIALS,34(17),4309-4326.
MLA He, Bing,et al."The transport pathways of polymer nanoparticles in MDCK epithelial cells".BIOMATERIALS 34.17(2013):4309-4326.
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