IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Hepatitis B virus genotypes, phylogeny and occult infection in a region with a high incidence of hepatocellular carcinoma in China
Fang, Zhong-Liao1; Zhuang, Hui1; Wang, Xue-Yan2; Ge, Xian-Min3; Harrison, Tim J.4
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2004-11-15
10期:22页:3264-3268
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
英文摘要

AIM: To determine the genotypes and phylogeny of hepatitis B viruses (HBVs) in asymptomatic HBV carriers, and the prevalence of occult HBV infection in Long An County, Guangxi Zhuang Autonomous Region, an area with a high incidence of hepatocellular carcinoma.

METHODS: A nested polymerase chain reaction (nPCR) was used for detection of HBV DNA in serum samples from 36 blood donors with asympmatic HBV infection, and in serum samples from 52 HBsAg negative family members of the children who did not receive hepatitis B vaccination in Long An County. PCR products were sequenced, and the genotype of each HBV sequence was determined by comparison with sequences of known genotypes in the GenBank and EMBL nucleotide databases using the BLAST programme. Phylogenetic trees were constructed by the quartet maximum likelihood analysis using the TreePuzzle software.

RESULTS: Twenty (55.56%) of 36 HBV asymptomatic carriers were positive for HBV DNA. They were all genotype C by comparison with sequences of known genotypes in the GenBank and EMBL nucleotide databases. The full-length HBV DNA sequence isolated from the sample No. 624 contained 3 215 bases. No interesting mutations were found in this isolate. The homology analysis showed that this strain was closer to the Vietnamese HBV genotype C strain, with a homology of 97%, compared its relation to the same genotype of HBV isolated in Shanghai. Six (11.5%) of the 52 HBsAg negative family members were positive for HBV DNA. A point mutation was found in the sample No. 37, resulting in the substitution of amino acid glycine to arginine in the "a" determinant. Other samples with positive HBV DNA did not have any unusual amino acid substitutions in or around the "a" determinant, and were attributed to the wild-type HBV.

CONCLUSION: The HBVs isolated from asymptomatic carriers of Long An County were all identified as genotype C, and the prevalence of occult HBV infection in the population of the county is as high as 11.5%. It is suggested that genotype C and persistent occult HBV infection may play an important role in the development of HCC in the county.

语种英语
WOS记录号WOS:000208318200007
项目编号H020920020190
资助机构Beijing Municipal Committee of Science and Technology
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67518
专题北京大学基础医学院_病原生物学系
北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med, Dept Microbiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Ctr Dis Prevent & Control Guangxi Zhuang Autonomo, Nanning 530021, Guangxi Zhuang, Peoples R China
3.Guangxi Worker Hosp, Nanning 530021, Guangxi Zhuang, Peoples R China
4.UCL, Ctr Hepatol, Royal Free & Univ Coll Med Sch, London, England
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GB/T 7714
Fang, Zhong-Liao,Zhuang, Hui,Wang, Xue-Yan,et al. Hepatitis B virus genotypes, phylogeny and occult infection in a region with a high incidence of hepatocellular carcinoma in China[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2004,10(22):3264-3268.
APA Fang, Zhong-Liao,Zhuang, Hui,Wang, Xue-Yan,Ge, Xian-Min,&Harrison, Tim J..(2004).Hepatitis B virus genotypes, phylogeny and occult infection in a region with a high incidence of hepatocellular carcinoma in China.WORLD JOURNAL OF GASTROENTEROLOGY,10(22),3264-3268.
MLA Fang, Zhong-Liao,et al."Hepatitis B virus genotypes, phylogeny and occult infection in a region with a high incidence of hepatocellular carcinoma in China".WORLD JOURNAL OF GASTROENTEROLOGY 10.22(2004):3264-3268.
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