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Comparison of alpha1-adrenergic receptor cell-membrane stationary phases prepared from expressed cell line and from rabbit hepatocytes
Wang, Yu; Yuan, Bingxiang; Deng, Xiuling; He, Langchong; Wang, Sicen; Zhang, Youyi; Han, Qide
关键词G-protein-coupled receptor cell-membrane chromatography cell-membrane stationary phase alpha(1A) adrenergic receptors immobilization
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2006-12-01
DOI10.1007/s00216-006-0859-6
386期:7-8页:2003-2011
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]RADIOLIGAND-BINDING ASSAY ; PROTEIN-COUPLED RECEPTOR ; AFFINITY-CHROMATOGRAPHY ; ALPHA(1)-ADRENOCEPTOR ; ALPHA(1A) ; SUBTYPE ; CLONING
英文摘要

A G-protein-coupled receptor-cell-membrane stationary phase (CMSP) has been prepared by immobilizing cell membranes on the surface of silica, as carrier. The resulting HEK293 alpha (1A) adrenoceptor cell-membrane stationary phase can be used for rapid on-line chromatographic determination of potential subtype-selective alpha (1) -adrenoceptor ligand-binding affinities for alpha (1) -adrenoceptor subtypes. The objective of the research was to study whether cell lines stably overexpressing subtype receptors could improve the sensitivity and specificity of cell-membrane chromatography (CMC) compared with use of homogenized tissue and cells in primary culture. Effects of mobile-phase ionic strength, sample concentration, and the presence of competitive agents on ligand-receptor interaction in CMSP were also evaluated. We found that cell lines stably overexpressing subtype receptors led to improved sensitivity and specificity in CMC. The technique leads to useful procedures-cell-membrane stationary phases may, for example, facilitate exploration of ligand-receptor interaction and determination of ligand-receptor binding affinity in initial screening and separation of lead compounds or active components in Chinese traditional natural medicine and herbs. This might eventually be an important contribution and an addition to our collection of techniques.

语种英语
WOS记录号WOS:000242144200008
引用统计
被引频次:34[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67526
专题北京大学第三临床医学院_心血管内科
作者单位1.Xi An Jiao Tong Univ, Sch Med, Dept Pharmacol, Xian 710061, Peoples R China
2.Xi An Jiao Tong Univ, Coll Pharm, Xian 710061, Peoples R China
3.Peking Univ, Third Hosp, Inst Vasc Med, Beijing 100083, Peoples R China
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GB/T 7714
Wang, Yu,Yuan, Bingxiang,Deng, Xiuling,et al. Comparison of alpha1-adrenergic receptor cell-membrane stationary phases prepared from expressed cell line and from rabbit hepatocytes[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2006,386(7-8):2003-2011.
APA Wang, Yu.,Yuan, Bingxiang.,Deng, Xiuling.,He, Langchong.,Wang, Sicen.,...&Han, Qide.(2006).Comparison of alpha1-adrenergic receptor cell-membrane stationary phases prepared from expressed cell line and from rabbit hepatocytes.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,386(7-8),2003-2011.
MLA Wang, Yu,et al."Comparison of alpha1-adrenergic receptor cell-membrane stationary phases prepared from expressed cell line and from rabbit hepatocytes".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 386.7-8(2006):2003-2011.
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