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学科主题: 临床医学
题名:
Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex
作者: Chen, Zhanguang1; Qian, Sihua1; Liu, Guoliang1; Chen, Xi2; Chen, Junhui3
关键词: Resonance light scattering (RLS) ; Shoulder peak ; Doxorubicin (DOX) ; Daunorubicin (DAU) ; DNA
刊名: MICROCHIMICA ACTA
发表日期: 2011-12-01
DOI: 10.1007/s00604-011-0692-2
卷: 175, 期:3-4, 页:217-223
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; CETYLTRIMETHYLAMMONIUM BROMIDE ; CAPILLARY-ELECTROPHORESIS ; HUMAN SERUM ; ASSAY ; CARDIOTOXICITY ; AGGREGATION ; ADRIAMYCIN ; SURFACTANT
英文摘要:

We have developed a resonance light scattering (RLS) quenching assay for the highly sensitive determination of doxorubicin (DOX) and daunorubicin (DAU). It is based on the reduction of the intensity of the shoulder of the RLS spectra at 443 nm. The intensity of the RLS of the ethidium-DNA system decrease linearly on addition of trace quantities of DOX or DAU within the concentration range of 0.008 to 12.0 mu g mL(-1) for DOX, and of 0.010 to 21.0 mu g mL(-1) for DAU. The detection limits are 3.0 and 5.0 ng mL(-1), respectively. The assay was successfully applied to the determination of DAU in synthetic and serum samples. Compared to the reported methods for anthracyclines, this assay displays higher sensitivity, lower detection limits, and a wider linear range.

语种: 英语
所属项目编号: 2006B35630009 ; YR09009
项目资助者: Guangdong Science and Technology Department ; Science Foundation of Shantou University
WOS记录号: WOS:000296924800002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67529
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
3.Peking Univ, Dept Radiol, Shenzhen Hosp, Shenzhen 518036, Peoples R China

Recommended Citation:
Chen, Zhanguang,Qian, Sihua,Liu, Guoliang,et al. Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex[J]. MICROCHIMICA ACTA,2011,175(3-4):217-223.
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