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学科主题临床医学
Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex
Chen, Zhanguang1; Qian, Sihua1; Liu, Guoliang1; Chen, Xi2; Chen, Junhui3
关键词Resonance light scattering (RLS) Shoulder peak Doxorubicin (DOX) Daunorubicin (DAU) DNA
刊名MICROCHIMICA ACTA
2011-12-01
DOI10.1007/s00604-011-0692-2
175期:3-4页:217-223
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]FLOW-INJECTION ANALYSIS ; NUCLEIC-ACIDS ; CETYLTRIMETHYLAMMONIUM BROMIDE ; CAPILLARY-ELECTROPHORESIS ; HUMAN SERUM ; ASSAY ; CARDIOTOXICITY ; AGGREGATION ; ADRIAMYCIN ; SURFACTANT
英文摘要

We have developed a resonance light scattering (RLS) quenching assay for the highly sensitive determination of doxorubicin (DOX) and daunorubicin (DAU). It is based on the reduction of the intensity of the shoulder of the RLS spectra at 443 nm. The intensity of the RLS of the ethidium-DNA system decrease linearly on addition of trace quantities of DOX or DAU within the concentration range of 0.008 to 12.0 mu g mL(-1) for DOX, and of 0.010 to 21.0 mu g mL(-1) for DAU. The detection limits are 3.0 and 5.0 ng mL(-1), respectively. The assay was successfully applied to the determination of DAU in synthetic and serum samples. Compared to the reported methods for anthracyclines, this assay displays higher sensitivity, lower detection limits, and a wider linear range.

语种英语
WOS记录号WOS:000296924800002
项目编号2006B35630009 ; YR09009
资助机构Guangdong Science and Technology Department ; Science Foundation of Shantou University
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67529
专题北京大学深圳医院
作者单位1.Shantou Univ, Dept Chem, Shantou 515063, Peoples R China
2.Guangdong Pharmaceut Univ, Guangzhou 510006, Guangdong, Peoples R China
3.Peking Univ, Dept Radiol, Shenzhen Hosp, Shenzhen 518036, Peoples R China
推荐引用方式
GB/T 7714
Chen, Zhanguang,Qian, Sihua,Liu, Guoliang,et al. Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex[J]. MICROCHIMICA ACTA,2011,175(3-4):217-223.
APA Chen, Zhanguang,Qian, Sihua,Liu, Guoliang,Chen, Xi,&Chen, Junhui.(2011).Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex.MICROCHIMICA ACTA,175(3-4),217-223.
MLA Chen, Zhanguang,et al."Highly sensitive determination of doxorubicin and daunorubicin based on their effect on the resonance light scattering signals of the ethidium-DNA complex".MICROCHIMICA ACTA 175.3-4(2011):217-223.
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