北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 神经生物学系  > 期刊论文
学科主题: 基础医学
题名:
DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS
作者: Liu, Y.1,2; Ye, Z.1,2; Yang, H.1,2; Zhou, L.1,2; Fan, D.3; He, S.1,2; Chui, D.1,2,3
关键词: hippocampal synaptosomes ; SNARE proteins ; cognitive impairment ; formaldehyde inhalation
刊名: NEUROSCIENCE
发表日期: 2010-09-01
DOI: 10.1016/j.neuroscience.2010.05.061
卷: 169, 期:3, 页:1248-1254
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: LOW-LEVEL FORMALDEHYDE ; HISTOLOGY TECHNICIANS ; PRESYNAPTIC PROTEINS ; CHEMICAL-SENSITIVITY ; WATER-MAZE ; EXPOSURE ; MEMORY ; MICE ; SENSITIZATION ; EXPRESSION
英文摘要:

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein) complex, a four-helical bundle composed of syntaxin1 and synaptosome-associated protein 25 (SNAP25) on the plasma membrane and synaptobrevin/VAMP2 (vesicle-associated membrane protein 2) on the vesicle membrane, plays a key role in synaptic exocytosis and facilitates neurotransmission. Disturbances of SNARE proteins were uncovered in some neurodegenerative diseases, neuroendocrine disturbances and even after environmental interventions. In the present study, we evaluated the effects of formaldehyde (FA) inhalation (13.5 +/- 1.5 ppm, twice 30-min each day for two rounds of 14 consecutive days) on learning and memory in Morris water maze and thereafter explored the SNARE protein levels in hippocampal synaptosomes. The formaldehyde-treated rats showed learning and memory impairment in escape latency and probe trials, without mobility disturbances in Morris water maze. Using western blotting assays, we detected the SNARE proteins in hippocampal synaptosomes and identified decrease of both SNAP25 and VAMP2 after formaldehyde treatment without significant changes of another SNARE protein, syntaxin 1, and synaptic vesicle marker, synaptophysin. Furthermore, the neuronal morphology and number detected in Nissl stain and western blotting assay of neurofilament-150 and synaptophysin were not affected after FA treatment. These results suggested that the specific decrease of SNAP25 and VAMP2 in hippocampal synaptosomes served as a potential contributing mechanism underlying learning and memory impairments after repetitive formaldehyde inhalation treatment. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30570533 ; 30670414 ; 30973145 ; 2006CB500705 ; 0060102A4031
项目资助者: National Natural Science Foundation of China ; National Hightech Research Developments program of China (973-project) ; National Hightech Research Developments program of China (863-project )
WOS记录号: WOS:000281109200028
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67541
Appears in Collections:基础医学院_神经生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS.pdf(672KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Key Lab Neurosci,Minist Educ,Minist Publ Hlth, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Neurol, Beijing 100191, Peoples R China

Recommended Citation:
Liu, Y.,Ye, Z.,Yang, H.,et al. DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS[J]. NEUROSCIENCE,2010,169(3):1248-1254.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Y.]'s Articles
[Ye, Z.]'s Articles
[Yang, H.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Y.]‘s Articles
[Ye, Z.]‘s Articles
[Yang, H.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace