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DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS
Liu, Y.1,2; Ye, Z.1,2; Yang, H.1,2; Zhou, L.1,2; Fan, D.3; He, S.1,2; Chui, D.1,2,3
关键词hippocampal synaptosomes SNARE proteins cognitive impairment formaldehyde inhalation
刊名NEUROSCIENCE
2010-09-01
DOI10.1016/j.neuroscience.2010.05.061
169期:3页:1248-1254
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者National Natural Science Foundation of China ; National Hightech Research Developments program of China (973-project) ; National Hightech Research Developments program of China (863-project ) ; National Natural Science Foundation of China ; National Hightech Research Developments program of China (973-project) ; National Hightech Research Developments program of China (863-project )
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]LOW-LEVEL FORMALDEHYDE ; HISTOLOGY TECHNICIANS ; PRESYNAPTIC PROTEINS ; CHEMICAL-SENSITIVITY ; WATER-MAZE ; EXPOSURE ; MEMORY ; MICE ; SENSITIZATION ; EXPRESSION
英文摘要

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein) complex, a four-helical bundle composed of syntaxin1 and synaptosome-associated protein 25 (SNAP25) on the plasma membrane and synaptobrevin/VAMP2 (vesicle-associated membrane protein 2) on the vesicle membrane, plays a key role in synaptic exocytosis and facilitates neurotransmission. Disturbances of SNARE proteins were uncovered in some neurodegenerative diseases, neuroendocrine disturbances and even after environmental interventions. In the present study, we evaluated the effects of formaldehyde (FA) inhalation (13.5 +/- 1.5 ppm, twice 30-min each day for two rounds of 14 consecutive days) on learning and memory in Morris water maze and thereafter explored the SNARE protein levels in hippocampal synaptosomes. The formaldehyde-treated rats showed learning and memory impairment in escape latency and probe trials, without mobility disturbances in Morris water maze. Using western blotting assays, we detected the SNARE proteins in hippocampal synaptosomes and identified decrease of both SNAP25 and VAMP2 after formaldehyde treatment without significant changes of another SNARE protein, syntaxin 1, and synaptic vesicle marker, synaptophysin. Furthermore, the neuronal morphology and number detected in Nissl stain and western blotting assay of neurofilament-150 and synaptophysin were not affected after FA treatment. These results suggested that the specific decrease of SNAP25 and VAMP2 in hippocampal synaptosomes served as a potential contributing mechanism underlying learning and memory impairments after repetitive formaldehyde inhalation treatment. (C) 2010 IBRO. Published by Elsevier Ltd. All rights reserved.

语种英语
所属项目编号30570533 ; 30670414 ; 30973145 ; 2006CB500705 ; 0060102A4031
资助者National Natural Science Foundation of China ; National Hightech Research Developments program of China (973-project) ; National Hightech Research Developments program of China (863-project ) ; National Natural Science Foundation of China ; National Hightech Research Developments program of China (973-project) ; National Hightech Research Developments program of China (863-project )
WOS记录号WOS:000281109200028
引用统计
被引频次:19[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67541
专题基础医学院_神经生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Key Lab Neurosci,Minist Educ,Minist Publ Hlth, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Dept Neurol, Beijing 100191, Peoples R China
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Liu, Y.,Ye, Z.,Yang, H.,et al. DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS[J]. NEUROSCIENCE,2010,169(3):1248-1254.
APA Liu, Y..,Ye, Z..,Yang, H..,Zhou, L..,Fan, D..,...&Chui, D..(2010).DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS.NEUROSCIENCE,169(3),1248-1254.
MLA Liu, Y.,et al."DISTURBANCES OF SOLUBLE N-ETHYLMALEIMIDE-SENSITIVE FACTOR ATTACHMENT PROTEINS IN HIPPOCAMPAL SYNAPTOSOMES CONTRIBUTE TO COGNITIVE IMPAIRMENT AFTER REPETITIVE FORMALDEHYDE INHALATION IN MALE RATS".NEUROSCIENCE 169.3(2010):1248-1254.
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