IR@PKUHSC  > 北京大学口腔医学院  > 牙周科
学科主题口腔医学
Aggregatibacter actinomycetemcomitans Infection Enhances Apoptosis In Vivo through a Caspase-3-Dependent Mechanism in Experimental Periodontitis
Kang, Jun1,2,3; Bezerra, Beatriz de Brito4; Pacios, Sandra1,6; Andriankaja, Oelisoa1; Li, Yu5; Tsiagbe, Vincent5; Schreiner, Helen5; Fine, Daniel H.5; Graves, Dana T.1
刊名INFECTION AND IMMUNITY
2012-06-01
DOI10.1128/IAI.06371-11
80期:6页:2247-2256
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases
资助者NIDCR ; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Brazil) ; NIDCR ; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Brazil)
研究领域[WOS]Immunology ; Infectious Diseases
关键词[WOS]GENERALIZED AGGRESSIVE PERIODONTITIS ; CYTOLETHAL DISTENDING TOXIN ; ALVEOLAR BONE LOSS ; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS ; DIABETES-MELLITUS ; LONGITUDINAL COHORT ; RAT MODEL ; DISEASES ; CELLS ; MICE
英文摘要

The purpose of this study was to test the hypothesis that diabetes aggravates periodontal destruction induced by Aggregatibacter actinomycetemcomitans infection. Thirty-eight diabetic and 33 normal rats were inoculated with A. actinomycetemcomitans and euthanized at baseline and at 4, 5, and 6 weeks after inoculation. Bone loss and the infiltration of polymorphonuclear leukocytes (PMNs) in gingival epithelium were measured in hematoxylin-eosin-stained sections. The induction of tumor necrosis factor alpha (TNF-alpha) was evaluated by immunohistochemistry and of apoptotic cells by a TUNEL (terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling) assay. After A. actinomycetemcomitans infection, the bone loss in diabetic rats was 1.7-fold and the PMN infiltration 1.6-fold higher than in normoglycemic rats (P < 0.05). The induction of TNF-alpha was 1.5-fold higher and of apoptotic cells was up to 3-fold higher in diabetic versus normoglycemic rats (P < 0.05). Treatment with a caspase-3 inhibitor significantly blocked noninflammatory cell apoptosis induced by A. actinomycetemcomitans infection in gingival epithelium and connective tissue (P < 0.05). These results provide new insight into how diabetes aggravates A. actinomycetemcomitans-induced periodontal destruction in rats by significantly increasing the inflammatory response, leading to increased bone loss and enhancing apoptosis of gingival epithelial and connective tissue cells through a caspase-3-dependent mechanism. Antibiotics had a more pronounced effect on many of these parameters in diabetic than in normoglycemic rats, suggesting a deficiency in the capacity of diabetic animals to resist infection.

语种英语
所属项目编号DE018307 ; DE017732
资助者NIDCR ; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Brazil) ; NIDCR ; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (Brazil)
WOS记录号WOS:000304387700032
Citation statistics
Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67550
Collection北京大学口腔医学院_牙周科
作者单位1.Univ Penn, Sch Dent Med, Dept Periodont, Philadelphia, PA 19104 USA
2.Peking Univ, Dept Periodontol, Sch Stomatol, Beijing 100871, Peoples R China
3.Peking Univ, Dept Periodontol, Hosp Stomatol, Beijing 100871, Peoples R China
4.Univ Estadual Campinas, Prosthodont & Periodont Dept, Piracicaba Dent Sch, Piracicaba, Brazil
5.Univ Med & Dent New Jersey, New Jersey Dent Sch, Dept Oral Biol, Newark, NJ 07103 USA
6.Univ Int Catalunya, Dept Periodontol, Sch Dent Med, Sant Cugat Del Valles, Spain
Recommended Citation
GB/T 7714
Kang, Jun,Bezerra, Beatriz de Brito,Pacios, Sandra,et al. Aggregatibacter actinomycetemcomitans Infection Enhances Apoptosis In Vivo through a Caspase-3-Dependent Mechanism in Experimental Periodontitis[J]. INFECTION AND IMMUNITY,2012,80(6):2247-2256.
APA Kang, Jun.,Bezerra, Beatriz de Brito.,Pacios, Sandra.,Andriankaja, Oelisoa.,Li, Yu.,...&Graves, Dana T..(2012).Aggregatibacter actinomycetemcomitans Infection Enhances Apoptosis In Vivo through a Caspase-3-Dependent Mechanism in Experimental Periodontitis.INFECTION AND IMMUNITY,80(6),2247-2256.
MLA Kang, Jun,et al."Aggregatibacter actinomycetemcomitans Infection Enhances Apoptosis In Vivo through a Caspase-3-Dependent Mechanism in Experimental Periodontitis".INFECTION AND IMMUNITY 80.6(2012):2247-2256.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Kang, Jun]'s Articles
[Bezerra, Beatriz de Brito]'s Articles
[Pacios, Sandra]'s Articles
百度学术
百度学术Similar articles in
[Kang, Jun]'s Articles
[Bezerra, Beatriz de Brito]'s Articles
[Pacios, Sandra]'s Articles
必应学术
必应学术Similar articles in
[Kang, Jun]'s Articles
[Bezerra, Beatriz de Brito]'s Articles
[Pacios, Sandra]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.