IR@PKUHSC  > 北京大学第二临床医学院  > 眼科
学科主题临床医学
siRNA targeting mammalian target of rapamycin (mTOR) attenuates experimental proliferative vitreoretinopathy
Zhou, Peng; Zhao, Ming-Wei; Li, Xiao-Xin; Yu, Wen-Zhen; Bian, Zong-Mei
关键词human retinal pigment epithelial cell mammalian target of rapamycin migration proliferative vitreoretinopathy small interfering RNA
刊名CURRENT EYE RESEARCH
2007-11-01
DOI10.1080/02713680701658323
32期:11页:973-984
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]EXPERIMENTAL-MODEL ; RPE CELLS ; IN-VITRO ; MIGRATION ; ALKYLPHOSPHOCHOLINES ; NEOVASCULARIZATION ; INHIBITION ; PATHWAY
英文摘要

Purpose: To investigate the effect of mammalian target of rapamycin (mTOR) specific siRNA on proliferative vitreoretinopathy (PVR). Methods: Cultured human retinal pigment epithelial (hRPE) cell line D407 was treated with three mTOR specific small interfering RNAs. Cell proliferation, attachment, spreading, and migration were performed. The impact of the mTOR specific siRNA on PVR was tested using a rabbit model in which PVR was induced by the injection of hRPE cells. Results: Decreasing mTOR expression by about 82% using small interfering RNA resulted in a significant decrease in cell spreading and migration. Whereas retinal detachment occurred in 100% of the control group animals, co-injection of the mTOR specific siRNA substantially reduced the severity and incidence (50%) of retinal detachments. Conclusions: Gene therapy with mTOR specific siRNA attenuates PVR in a rabbit model of the disease. This may be a new approach to preventing PVR in humans.

语种英语
WOS记录号WOS:000251506500007
Citation statistics
Cited Times:16[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67552
Collection北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 10044, Peoples R China
2.Univ Michigan, Dept Ophthalmol, Ann Arbor, MI USA
Recommended Citation
GB/T 7714
Zhou, Peng,Zhao, Ming-Wei,Li, Xiao-Xin,et al. siRNA targeting mammalian target of rapamycin (mTOR) attenuates experimental proliferative vitreoretinopathy[J]. CURRENT EYE RESEARCH,2007,32(11):973-984.
APA Zhou, Peng,Zhao, Ming-Wei,Li, Xiao-Xin,Yu, Wen-Zhen,&Bian, Zong-Mei.(2007).siRNA targeting mammalian target of rapamycin (mTOR) attenuates experimental proliferative vitreoretinopathy.CURRENT EYE RESEARCH,32(11),973-984.
MLA Zhou, Peng,et al."siRNA targeting mammalian target of rapamycin (mTOR) attenuates experimental proliferative vitreoretinopathy".CURRENT EYE RESEARCH 32.11(2007):973-984.
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