IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
EGFP expression controlled by GFAP promoter in mesenchymal cells: An efficient tool for glial lineage selection and transplantation
Zhang, PX; He, XJ; Zhao, FQ; Du, C; Fu, ZG; Zhang, DY; Zhang, HB; Jiang, BG
刊名ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY
2005
DOI10.1081/BIO-200066628
33期:3页:307-317
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Biotechnology & Applied Microbiology ; Engineering ; Materials Science
关键词[WOS]MARROW STROMAL CELLS ; NERVE REGENERATION ; IN-VITRO ; FUSION
英文摘要

In order to demonstrate a new method to label and select enough glial cells from induced MSCs to provide cells for cell therapy, MSCs were induced with Beta-mercaptoethanol followed by retinoic acid, forskolin, basic-FGF, PDGF and heregulin. Induced MSCs were transfected with reconstructed vector pGFAP-EGFP by inserting GFAP promotor into pEGFP-N3 to substitute CMV promotor. Living cells against G418 were enriched and checked by flowcytometry. EGFP expressing cells were sorted and used for transplantation in vivo. Immunoelectronmicroscopy was accomplished using anti-EGFP to relocalize the transplanted cells. Almost all MSCs took on phenotypes of glial cells after induction, expressing S100 and GFAP. The EGFP expression rate of survived MSCs against G418 was 82.74%. Glial cells expressing EGFP accumulated mainly around the damaged nerve fibers. MSCs were relocalized by immunoelectronmicroscopy and remyelination was observed. EGFP expression controlled by GFAP promoter in mesenchymal cells was an efficient toot for glial lineage selection and transplantation. Induced MSCs can promote nerve regeneration by participating remyelination.

语种英语
WOS记录号WOS:000231417000006
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67560
Collection北京大学第二临床医学院
作者单位Peking Univ, Peoples Hosp, Dept Orthoped & Trauma, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Zhang, PX,He, XJ,Zhao, FQ,et al. EGFP expression controlled by GFAP promoter in mesenchymal cells: An efficient tool for glial lineage selection and transplantation[J]. ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,2005,33(3):307-317.
APA Zhang, PX.,He, XJ.,Zhao, FQ.,Du, C.,Fu, ZG.,...&Jiang, BG.(2005).EGFP expression controlled by GFAP promoter in mesenchymal cells: An efficient tool for glial lineage selection and transplantation.ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY,33(3),307-317.
MLA Zhang, PX,et al."EGFP expression controlled by GFAP promoter in mesenchymal cells: An efficient tool for glial lineage selection and transplantation".ARTIFICIAL CELLS BLOOD SUBSTITUTES AND BIOTECHNOLOGY 33.3(2005):307-317.
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