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Abnormal subcellular localization of AQP5 and downregulated AQP5 protein in parotid glands of streptozotocin-induced diabetic rats
Wang, Di; Yuan, Zhenfang4; Inoue, Noriko; Cho, Gota2; Shono, Masayuki3; Ishikawa, Yasuko1
关键词Type 1 diabetes Aquaporin-5 Lipid raft Parotid gland Xerostomia Translocation
刊名BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
2011-05-01
DOI10.1016/j.bbagen.2011.01.013
1810期:5页:543-554
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]APICAL PLASMA-MEMBRANE ; LIPID RAFTS ; SALIVARY SECRETION ; ACINAR-CELLS ; HYPERTONIC INDUCTION ; SIGNAL-TRANSDUCTION ; WATER CHANNELS ; AQUAPORIN-5 ; ACTIVATION ; IDENTIFICATION
英文摘要

Background: The mechanisms underlying diabetic xerostomia have not been clarified in relation with aquaporin-5 (AQP5) subcellular localization in salivary glands.

Methods: Western blotting, real-time PCR, and immunocytochemistry were used to analyse AQP5 protein levels and mRNA expression. AQP5 protein levels were measured in the apical plasma membrane (APM) and detergent-insoluble fraction prepared from streptozotocin-diabetic rat parotid glands.

Results: Despite an increase in AQP5 mRNA, AQP5 protein levels were decreased in diabetic parotid glands compared with controls. Immunohistochemical studies indicated that AQP5, under unstimulated conditions, colocalised with flotillin-2 and GM1 with a diffuse pattern in the apical cytoplasm of acinar and duct cells in both control and diabetic rats. Ten minutes after intravenous injection of muscarinic agonist cevimeline. AQP5 was dramatically increased together with flotillin-2 and GM1 in the APM of parotid acinar and duct cells of control but not diabetic rats. Sixty minutes after injection, AQP5 was located in a diffuse pattern in the apical cytoplasm in both rats. Treatment of the parotid tissues with cevimeline for 10 min increased the Triton X-100 solubility of AQP5 in control but not diabetic rats. Administration of insulin to diabetic rats tended to restore the cevimeline-induced translocation of AQP5.

Conclusion: Lack of AQP5 translocation in the salivary gland in response to a muscarinic agonist and downregulation of AQP5 protein might lead to diabetic xerostomia.

General significance: Cevimeline is useful to cure diabetic xerostomia under insulin administration. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000290067900007
资助机构Ministry of Education, Science, Sports, and Culture of japan ; Daiichi Pharmaceutical Company (Tokyo, Japan)
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67573
专题北京大学第一临床医学院_大内科
北京大学第一临床医学院_内分泌内科
作者单位1.Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dent Pharmacol Branch,Dept Med Pharmacol, Tokushima 7708504, Japan
2.Univ Tokushima, Grad Sch, Inst Hlth Biosci, Dept Dent Anaesthesiol, Tokushima 7708504, Japan
3.Univ Tokushima, Grad Sch, Inst Hlth Biosci, Support Ctr Adv Med Sci, Tokushima 7708504, Japan
4.Peking Univ, Hosp 1, Dept Internal Med, Beijing 100034, Peoples R China
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GB/T 7714
Wang, Di,Yuan, Zhenfang,Inoue, Noriko,et al. Abnormal subcellular localization of AQP5 and downregulated AQP5 protein in parotid glands of streptozotocin-induced diabetic rats[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2011,1810(5):543-554.
APA Wang, Di,Yuan, Zhenfang,Inoue, Noriko,Cho, Gota,Shono, Masayuki,&Ishikawa, Yasuko.(2011).Abnormal subcellular localization of AQP5 and downregulated AQP5 protein in parotid glands of streptozotocin-induced diabetic rats.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1810(5),543-554.
MLA Wang, Di,et al."Abnormal subcellular localization of AQP5 and downregulated AQP5 protein in parotid glands of streptozotocin-induced diabetic rats".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1810.5(2011):543-554.
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