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学科主题临床医学
Dendritic cells pulsed with alpha-fetoprotein and mutant P53 fused gene induce bi-targeted cytotoxic T lymphocyte response against hepatic carcinoma
Ren, Jun1; Jia, Jun1; Zhang, Hongmei2; Zhang, Liwang2; Ma, Bo1; Jiang, Hanfang1; Di, Lijun1; Song, Guohong1; Yu, Jing1
刊名CANCER SCIENCE
2008-07-01
DOI10.1111/j.1349-7006.2008.00820.x
99期:7页:1420-1426
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]COLONY-STIMULATING FACTOR ; HEPATOCELLULAR-CARCINOMA ; TUMOR-CELLS ; ANTITUMOR IMMUNITY ; PERIPHERAL-BLOOD ; TOTAL RNA ; IN-VITRO ; VACCINATION ; CANCER ; IMMUNOTHERAPY
英文摘要

Dendritic cell (DC)-based immunotherapy is rapidly emerging as a promising treatment in cancer therapy. We had previously shown that DC pulsed with either defined mRNA of tumor antigen (Ag) such as alpha-fetoprotein (AFP), or total RNA of hepatocellular carcinoma (HCC) could elicit Ag-specific cytotoxic T lymphocyte (CTL) response. Therefore, we suggested a novel DC-based therapeutic method, in which DCs derived from CD34(+) cells enriched peripheral blood mononuclear cells were pulsed with liposome-coated AFP and mutant P53 (mtP53) fused gene pEGFP-C3/AFP-mtP53 to induce bi-targeted specific CTL responses against HCC. Three different genotype HCC cell lines, HepG2 (human histocompatibility leukocyte antigens (HLA) A2 positive, AFP expressing positive, P53 expressing negative), SMMC7721 (HLA A2 positive, neither AFP nor P53 expressing positive), and HMCC97 (HLA A2 positive, both AFP and P53 expressing positive) were selected as targets for CTL responses. An important finding was that DCs pulsed with the liposome-coated fused gene could evoke more intensive bi-targeted Ag-specific CTL responses against HMCC97 than DCs pulsed with either AFP or P53 single gene (P < 0.05). This experimental therapeutic model provides a new promising cytotherapeutic approach, in that DCs pulsed with the fused gene of different Ags might induce more extensive multitargeted antitumor immunity.

语种英语
WOS记录号WOS:000256351900018
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67587
专题北京大学临床肿瘤学院_肿瘤内科
北京大学临床肿瘤学院_乳腺肿瘤内科
北京大学临床肿瘤学院_移植与免疫治疗病区
北京大学临床肿瘤学院_生物诊断与治疗中心
作者单位1.Peking Univ, Sch Oncol, Beijing Canc Hosp, Dept Med Oncol, Beijing 100036, Peoples R China
2.Fourth Mil Med Univ, Dept Clin Oncol, Xi Jing Hosp, Xian 710032, Peoples R China
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GB/T 7714
Ren, Jun,Jia, Jun,Zhang, Hongmei,et al. Dendritic cells pulsed with alpha-fetoprotein and mutant P53 fused gene induce bi-targeted cytotoxic T lymphocyte response against hepatic carcinoma[J]. CANCER SCIENCE,2008,99(7):1420-1426.
APA Ren, Jun.,Jia, Jun.,Zhang, Hongmei.,Zhang, Liwang.,Ma, Bo.,...&Yu, Jing.(2008).Dendritic cells pulsed with alpha-fetoprotein and mutant P53 fused gene induce bi-targeted cytotoxic T lymphocyte response against hepatic carcinoma.CANCER SCIENCE,99(7),1420-1426.
MLA Ren, Jun,et al."Dendritic cells pulsed with alpha-fetoprotein and mutant P53 fused gene induce bi-targeted cytotoxic T lymphocyte response against hepatic carcinoma".CANCER SCIENCE 99.7(2008):1420-1426.
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