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学科主题: 临床医学
题名:
Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells
作者: Salmiheimo, Aino N. E.1,2; Mustonen, Harri K.1,2; Vainionpaa, Sanna A. A.1,2; Shen, Zhanlong3; Kemppainen, Esko A. J.1,2; Seppanen, Hanna E.1,2; Puolakkainen, Pauli A.1,2
关键词: pancreatic adenocarcinoma ; M1 ; M2 ; macrophages ; invasion
刊名: JOURNAL OF CANCER
发表日期: 2016
DOI: 10.7150/jca.12923
卷: 7, 期:1, 页:42-49
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TUMOR-ASSOCIATED MACROPHAGES ; ACTIVATED MACROPHAGES ; ROLES ; GROWTH ; MICROENVIRONMENT ; POLARIZATION ; PROGRESSION ; METASTASIS ; PROFILES ; PROMOTE
英文摘要:

Recent studies suggest that pro-inflammatory type M1 macrophages inhibit tumor progression and that anti-inflammatory M2 macrophages enhance it. The aim of this study was to examine the interaction of type M1 and M2 macrophages with pancreatic cancer cells. We studied the migration rate of fluorescein stained pancreatic cancer cells on Matrigel cultured alone or with Granulocyte- Macrophage Colony Stimulating Factor (GM-CSF) differentiated macrophages or with Macrophage Colony Stimulating Factor (M-CSF) differentiated macrophages, skewing the phenotype towards pro- and anti-inflammatory direction, respectively. Macrophage differentiation was assessed with flow cytometry and the cytokine secretion in cell cultures with cytokine array. Both GM-CSF and M-CSF differentiated macrophages increased the migration rate of primary pancreatic adenocarcinoma cell line (MiaPaCa-2) and metastatic cell line (HPAF-II). Stimulation with IL6 or IL4+ LPS reversed the macrophages′ increasing effect on the migration rate of Mi-aPaCa-2 completely and partly of HPAF-II. Co-culture with MiaPaCa-2 reduced the inflammatory cytokine secretion of GM-CSF differentiated macrophages. Co-culture of macrophages with pancreatic cancer cells seem to change the inflammatory cytokine profile of GM-CSF differentiated macrophages and this might explain why also GM-CSF differentiated macrophages promoted the invasion. Adding IL6 or IL4+ LPS to the cell culture with MiaPaCa-2 and GM-CSF or M-CSF differentiated macrophages increased the secretion of inflammatory cytokines and this could contribute to the reversion of the macrophage induced increase of cancer cell migration rate.

语种: 英语
项目资助者: Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds ; Biomedicum Helsinki Foundation ; Finnish Medical Foundation
WOS记录号: WOS:000366281500007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67626
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Helsinki Univ Hosp, Dept Surg, Helsinki, Finland
2.Univ Helsinki, SF-00130 Helsinki, Finland
3.Peking Univ, Peoples Hosp, Dept Surg Gastroenterol, Beijing 100871, Peoples R China

Recommended Citation:
Salmiheimo, Aino N. E.,Mustonen, Harri K.,Vainionpaa, Sanna A. A.,et al. Increasing the Inflammatory Competence of Macrophages with IL-6 or with Combination of IL-4 and LPS Restrains the Invasiveness of Pancreatic Cancer Cells[J]. JOURNAL OF CANCER,2016,7(1):42-49.
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