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学科主题: 药学
题名:
Development of a Sensitive High-Performance Liquid Chromatographic Method with Simple Extraction for Simultaneous Determination of Huperzine A and Huperzine B in the Species Containing Lycopodium Alkaloids
作者: Zhang, Yanqing1,2,3; Xie, Junbo3,4; Chen, Wen-Qian2; Zhou, Tian-Yan2; Lu, Wei1,2
刊名: JOURNAL OF AOAC INTERNATIONAL
发表日期: 2009-07-01
卷: 92, 期:4, 页:1060-1063
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Food Science & Technology
研究领域[WOS]: Chemistry ; Food Science & Technology
关键词[WOS]: RAT PLASMA ; ACETYLCHOLINESTERASE
英文摘要:

A sensitive HPLC method with simple extraction was developed for simultaneous determination of huperzine A (HupA) and huperzine B (HupB) in Huperzia serrata, H. crispata, H. miyoshiana, and Lycopodiastrum casuarinoides. In order to avoid conventional multiple-step and time-consuming sample preparation methods, direct reflux extraction with alkaline chloroform was adopted. The quantitative determination was conducted by reversed-phase HPLC with a photodiode array detector set at 308 nm. Separation was performed on a Luna C18 column (250 x 4.6 mm id, 5 pm) with methanol-0.2% aqueous acetic acid (18 + 82, v/v) mobile phase. The method was validated for accuracy, reproducibility, precision, and limits of detection and quantification. Quantification of the two active compounds in the samples was performed by this newly developed method, and the content of HupA and HupB varied substantially among four different species. The satisfactory results indicated that the developed method can readily be utilized for quality control of the species of Huperziaceae and Lycopodiaceae containing the two compounds.

语种: 英语
WOS记录号: WOS:000269248500009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67628
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Peking Univ, Dept Pharmaceut, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
3.Tianjin Key Lab Food Biotechnol, Tianjin 300134, Peoples R China
4.Tianjin Univ Commerce, Dept Pharmaceut Engn, Tianjin 300134, Peoples R China

Recommended Citation:
Zhang, Yanqing,Xie, Junbo,Chen, Wen-Qian,et al. Development of a Sensitive High-Performance Liquid Chromatographic Method with Simple Extraction for Simultaneous Determination of Huperzine A and Huperzine B in the Species Containing Lycopodium Alkaloids[J]. JOURNAL OF AOAC INTERNATIONAL,2009,92(4):1060-1063.
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