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学科主题临床医学
Targeted delivery of non-viral vectors to cartilage in vivo using a chondrocyte-homing peptide identified by phage display
Pi, Yanbin1; Zhang, Xin1; Shi, Junjun1; Zhu, Jinxian1; Chen, Wenqing1; Zhang, Chenguang2; Gao, Weiwei2; Zhou, Chunyan2; Ao, Yingfang1
关键词Phage display Cartilage-targeting Gene therapy Peptide-modified polyethylenimine
刊名BIOMATERIALS
2011-09-01
DOI10.1016/j.biomaterials.2011.05.017
32期:26页:6324-6332
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]Engineering ; Materials Science
关键词[WOS]GENE-THERAPY ; OSTEOARTHRITIS ; NANOPARTICLES ; REPAIR ; KNEE ; JOINT ; CELLS ; HIP
英文摘要

Gene therapy is a promising method for osteoarthritis and cartilage injury. However, specifically delivering target genes into chondrocytes is a great challenge because of their non-vascularity and the dense extracellular matrix of cartilage. In our study, we identified a chondrocyte-affinity peptide (CAP, DWRVIIPPRPSA) by phage display technology. Subsequent analysis suggests that the peptide can efficiently interact specifically with chondrocytes without any species specificity. Polyethylenimine (PEI) was covalently modified with CAP to construct a non-viral vector for cartilage-targeted therapy. To investigate the cartilage-targeting property of the CAP-modified vector, FITC-labeled CAP conjugated PEI/DNA particles were injected into rabbit knee joints, and visualized under confocal microscope. Higher concentrations of CAP-modified vector were detected in the cartilage and specifically taken up by chondrocytes compared with a randomly scrambled peptide (SP)-modified vector. To evaluate cartilage-targeting transfection efficiency, the GFP and luciferase genes were delivered into knee joints using CAP- and SP-modified PEI. Cartilage transfections mediated by CAP-modified PEI were much more efficient and specific than those by SP-modified PEI. This result suggests that CAP-modified PEI could be used as a specific cartilage-targeting vector for cartilage disorders. (C) 2011 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000292904100039
项目编号2007AA021809
资助机构National High Technology Research and Development Program of China
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67636
专题北京大学第三临床医学院_运动医学研究所
北京大学基础医学院
北京大学第三临床医学院_骨科
作者单位1.Peking Univ, Inst Sports Med, Hosp 3, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biochem & Mol Biol, Beijing 100191, Peoples R China
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Pi, Yanbin,Zhang, Xin,Shi, Junjun,et al. Targeted delivery of non-viral vectors to cartilage in vivo using a chondrocyte-homing peptide identified by phage display[J]. BIOMATERIALS,2011,32(26):6324-6332.
APA Pi, Yanbin.,Zhang, Xin.,Shi, Junjun.,Zhu, Jinxian.,Chen, Wenqing.,...&Ao, Yingfang.(2011).Targeted delivery of non-viral vectors to cartilage in vivo using a chondrocyte-homing peptide identified by phage display.BIOMATERIALS,32(26),6324-6332.
MLA Pi, Yanbin,et al."Targeted delivery of non-viral vectors to cartilage in vivo using a chondrocyte-homing peptide identified by phage display".BIOMATERIALS 32.26(2011):6324-6332.
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