IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Proteomic profiling reveals comprehensive insights into adrenergic receptor-mediated hypertrophy in neonatal rat cardiomyocytes
Li, Zijian1,2; Liu, Ning3,4; Zhang, Li-Shu5; Gong, Kaizheng1,2; Cai, Yuanbin1,2; Gao, Wei1,2; Liu, Zhiqiang4; Liu, Shuying4; Han, Qide1,2; Zhang, Youyi1,2
关键词Adrenergic receptor Cardiac hypertrophy
刊名PROTEOMICS CLINICAL APPLICATIONS
2009-12-01
DOI10.1002/prca.200900029
3期:12页:1407-1421
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
资助者Major State Basic Research Development Program of People&prime ; s Republic of China ; National Science Foundation of China ; Chinese Ministry of Education ; Major State Basic Research Development Program of People&prime ; s Republic of China ; National Science Foundation of China ; Chinese Ministry of Education
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]CARDIAC-HYPERTROPHY ; OXIDATIVE STRESS ; ANGIOTENSIN-II ; HEART-FAILURE ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; PRESSURE-OVERLOAD ; TROPONIN-T ; IN-VITRO ; PATHWAYS ; CELLS
英文摘要

Myocardial adrenergic receptors (ARs) play important roles in cardiac hypertrophy. However, the detailed molecular mechanism of AR-mediated cardiac hypertrophy remains elusive to date. To gain full insight into how ARs are involved in the regulation of cardiac hypertrophy, protein expression profiling was performed with comparative proteomics approach on neonatal rat cardiomyocytes. Forty-six proteins were identified as differentially expressed in hypertrophic cardiomyocytes induced by AR stimulation. To better understand the biological significance of the obtained proteomic data, we utilized the ingenuity pathway analysis tool to construct biological networks and analyze function and pathways that might associate with AR-mediated cardiac hypertrophy. Pathway analysis strongly suggested that ROS may be involved in the development of AR-mediated cardiac hypertrophy, which was then confirmed by further experimentation. The results showed that a marked increase in ROS production was detected in AR-mediated cardiac hypertrophy and blocking of ROS production significantly inhibited AR-mediated cardiac hypertrophy. We further proved that the ROS production was through NADPH oxidase or the mitochondrial electron transport chain and this ROS accumulation resulted in activation of extracellular signal-regulated kinase 1/2 leading to AR-mediated cardiac hypertrophy. These experimental results support the hypothesis, from the ingenuity pathway analysis, that AR-mediated cardiac hypertrophy is associated with the dysregulation of a complicated oxidative stress-regulatory network. In conclusion, our results provide a basis for understanding the detailed molecular mechanisms of AR-mediated cardiac hypertrophy.

语种英语
所属项目编号2006CB503806 ; 30400552 ; 30821001 ; 20675079 ; NO 307001
资助者Major State Basic Research Development Program of People&prime ; s Republic of China ; National Science Foundation of China ; Chinese Ministry of Education ; Major State Basic Research Development Program of People&prime ; s Republic of China ; National Science Foundation of China ; Chinese Ministry of Education
WOS记录号WOS:000273184700006
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67637
专题北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100191, Peoples R China
2.Minist Educ, Key Lab Mol Cardiol, Beijing, Peoples R China
3.Jilin Univ, Changchun 130023, Peoples R China
4.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun Ctr Mass Spectrometry, Changchun 130022, Peoples R China
5.Univ Texas SW Med Ctr Dallas, Dept Dermatol, Dallas, TX 75390 USA
推荐引用方式
GB/T 7714
Li, Zijian,Liu, Ning,Zhang, Li-Shu,et al. Proteomic profiling reveals comprehensive insights into adrenergic receptor-mediated hypertrophy in neonatal rat cardiomyocytes[J]. PROTEOMICS CLINICAL APPLICATIONS,2009,3(12):1407-1421.
APA Li, Zijian.,Liu, Ning.,Zhang, Li-Shu.,Gong, Kaizheng.,Cai, Yuanbin.,...&Zhang, Youyi.(2009).Proteomic profiling reveals comprehensive insights into adrenergic receptor-mediated hypertrophy in neonatal rat cardiomyocytes.PROTEOMICS CLINICAL APPLICATIONS,3(12),1407-1421.
MLA Li, Zijian,et al."Proteomic profiling reveals comprehensive insights into adrenergic receptor-mediated hypertrophy in neonatal rat cardiomyocytes".PROTEOMICS CLINICAL APPLICATIONS 3.12(2009):1407-1421.
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