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学科主题: 临床医学
题名:
Proteomic profiling reveals comprehensive insights into adrenergic receptor-mediated hypertrophy in neonatal rat cardiomyocytes
作者: Li, Zijian1,2; Liu, Ning3,4; Zhang, Li-Shu5; Gong, Kaizheng1,2; Cai, Yuanbin1,2; Gao, Wei1,2; Liu, Zhiqiang4; Liu, Shuying4; Han, Qide1,2; Zhang, Youyi1,2
关键词: Adrenergic receptor ; Cardiac hypertrophy
刊名: PROTEOMICS CLINICAL APPLICATIONS
发表日期: 2009-12-01
DOI: 10.1002/prca.200900029
卷: 3, 期:12, 页:1407-1421
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: CARDIAC-HYPERTROPHY ; OXIDATIVE STRESS ; ANGIOTENSIN-II ; HEART-FAILURE ; ALPHA(1)-ADRENOCEPTOR SUBTYPES ; PRESSURE-OVERLOAD ; TROPONIN-T ; IN-VITRO ; PATHWAYS ; CELLS
英文摘要:

Myocardial adrenergic receptors (ARs) play important roles in cardiac hypertrophy. However, the detailed molecular mechanism of AR-mediated cardiac hypertrophy remains elusive to date. To gain full insight into how ARs are involved in the regulation of cardiac hypertrophy, protein expression profiling was performed with comparative proteomics approach on neonatal rat cardiomyocytes. Forty-six proteins were identified as differentially expressed in hypertrophic cardiomyocytes induced by AR stimulation. To better understand the biological significance of the obtained proteomic data, we utilized the ingenuity pathway analysis tool to construct biological networks and analyze function and pathways that might associate with AR-mediated cardiac hypertrophy. Pathway analysis strongly suggested that ROS may be involved in the development of AR-mediated cardiac hypertrophy, which was then confirmed by further experimentation. The results showed that a marked increase in ROS production was detected in AR-mediated cardiac hypertrophy and blocking of ROS production significantly inhibited AR-mediated cardiac hypertrophy. We further proved that the ROS production was through NADPH oxidase or the mitochondrial electron transport chain and this ROS accumulation resulted in activation of extracellular signal-regulated kinase 1/2 leading to AR-mediated cardiac hypertrophy. These experimental results support the hypothesis, from the ingenuity pathway analysis, that AR-mediated cardiac hypertrophy is associated with the dysregulation of a complicated oxidative stress-regulatory network. In conclusion, our results provide a basis for understanding the detailed molecular mechanisms of AR-mediated cardiac hypertrophy.

语种: 英语
所属项目编号: 2006CB503806 ; 30400552 ; 30821001 ; 20675079 ; NO 307001
项目资助者: Major State Basic Research Development Program of People&prime ; s Republic of China ; National Science Foundation of China ; Chinese Ministry of Education
WOS记录号: WOS:000273184700006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67637
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100191, Peoples R China
2.Minist Educ, Key Lab Mol Cardiol, Beijing, Peoples R China
3.Jilin Univ, Changchun 130023, Peoples R China
4.Chinese Acad Sci, Changchun Inst Appl Chem, Changchun Ctr Mass Spectrometry, Changchun 130022, Peoples R China
5.Univ Texas SW Med Ctr Dallas, Dept Dermatol, Dallas, TX 75390 USA

Recommended Citation:
Li, Zijian,Liu, Ning,Zhang, Li-Shu,et al. Proteomic profiling reveals comprehensive insights into adrenergic receptor-mediated hypertrophy in neonatal rat cardiomyocytes[J]. PROTEOMICS CLINICAL APPLICATIONS,2009,3(12):1407-1421.
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