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IR@PKUHSC  > 北京大学口腔医学院  > 第二门诊部  > 期刊论文
学科主题: 口腔医学
题名:
The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs
作者: Hu, Hongcheng1,2; Pu, Yinfei1,2; Lu, Songhe1,2; Zhang, Kuo3; Guo, Yuan1,2; Lu, Hui1,2; Li, Deli1,2; Li, Xuefen2; Li, Zichen4; Wu, Yuwei1,2; Tang, Zhihui1,2
关键词: adiponectin ; BMP2 (bone morphogenetic protein 2) ; dental material ; mesenchymal stem cells ; beagle dog
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2015-10-01
DOI: 10.3390/ijms161024946
卷: 16, 期:10, 页:24946-24964
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: BONE MORPHOGENETIC PROTEIN ; OSTEOBLAST DIFFERENTIATION ; SIGNALING PATHWAY ; ADIPONECTIN ; METABOLISM ; SURGERY ; REPAIR ; MICE
英文摘要:

A plastic and biodegradable bone substitute consists of poly (l-lactic-co-glycolic) acid and 30 wt % -tricalcium phosphate has been previously fabricated, but its osteogenic capability required further improvement. We investigated the use of globular adiponectin (gAPN) as an anabolic agent for tissue-engineered bone using this scaffold. A qualitative analysis of the bone regeneration process was carried out using CT and histological analysis 12 weeks after implantation. CBCT (Cone Beam Computed Tomography) superimposition was used to characterise the effect of the different treatments on bone formation. In this study, we also explored adiponectin′s (APN) influence on primary cultured human jaw bone marrow mesenchymal stem cells gene expressions involved in the osteogenesis. We found that composite scaffolds loaded with gAPN or bone morphogenetic protein 2 (BMP2) exhibited significantly increased bone formation and mineralisation following 12 weeks in the extraction sockets of beagle dogs, as well as enhanced expression of osteogenic markers. In vitro investigation revealed that APN also promoted osteoblast differentiation of primary cultured human jaw bone marrow mesenchymal stem cells (h-JBMMSCs), accompanied by increased activity of alkaline phosphatase, greater mineralisation, and production of the osteoblast-differentiated genes osteocalcin, bone sialoprotein and collagen type I, which was reversed by APPL1 siRNA. Therefore, the composite scaffold loaded with APN exhibited superior activity for guided bone regeneration compared with blank control or Bio-Oss((R)) (a commercially available product). The composite scaffold with APN has significant potential for clinical applications in bone tissue engineering.

语种: 英语
所属项目编号: 81300851 ; SKLODd2009001
项目资助者: National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; Peking University School of Stamotology Youth Fund
WOS记录号: WOS:000364232100095
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67639
Appears in Collections:北京大学口腔医学院_第二门诊部_期刊论文

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作者单位: 1.Peking Univ, Dent Ctr 2, Sch & Hosp Stomatol, Beijing 100101, Peoples R China
2.Peking Univ, Natl Engn Lab Digital & Mat Technol Stomatol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Dept Lab Anim Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Dept Polymer Sci & Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China

Recommended Citation:
Hu, Hongcheng,Pu, Yinfei,Lu, Songhe,et al. The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2015,16(10):24946-24964.
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