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学科主题口腔医学
The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs
Hu, Hongcheng1,2; Pu, Yinfei1,2; Lu, Songhe1,2; Zhang, Kuo3; Guo, Yuan1,2; Lu, Hui1,2; Li, Deli1,2; Li, Xuefen2; Li, Zichen4; Wu, Yuwei1,2; Tang, Zhihui1,2
关键词adiponectin BMP2 (bone morphogenetic protein 2) dental material mesenchymal stem cells beagle dog
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
2015-10-01
DOI10.3390/ijms161024946
16期:10页:24946-24964
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]BONE MORPHOGENETIC PROTEIN ; OSTEOBLAST DIFFERENTIATION ; SIGNALING PATHWAY ; ADIPONECTIN ; METABOLISM ; SURGERY ; REPAIR ; MICE
英文摘要

A plastic and biodegradable bone substitute consists of poly (l-lactic-co-glycolic) acid and 30 wt % -tricalcium phosphate has been previously fabricated, but its osteogenic capability required further improvement. We investigated the use of globular adiponectin (gAPN) as an anabolic agent for tissue-engineered bone using this scaffold. A qualitative analysis of the bone regeneration process was carried out using CT and histological analysis 12 weeks after implantation. CBCT (Cone Beam Computed Tomography) superimposition was used to characterise the effect of the different treatments on bone formation. In this study, we also explored adiponectin′s (APN) influence on primary cultured human jaw bone marrow mesenchymal stem cells gene expressions involved in the osteogenesis. We found that composite scaffolds loaded with gAPN or bone morphogenetic protein 2 (BMP2) exhibited significantly increased bone formation and mineralisation following 12 weeks in the extraction sockets of beagle dogs, as well as enhanced expression of osteogenic markers. In vitro investigation revealed that APN also promoted osteoblast differentiation of primary cultured human jaw bone marrow mesenchymal stem cells (h-JBMMSCs), accompanied by increased activity of alkaline phosphatase, greater mineralisation, and production of the osteoblast-differentiated genes osteocalcin, bone sialoprotein and collagen type I, which was reversed by APPL1 siRNA. Therefore, the composite scaffold loaded with APN exhibited superior activity for guided bone regeneration compared with blank control or Bio-Oss((R)) (a commercially available product). The composite scaffold with APN has significant potential for clinical applications in bone tissue engineering.

语种英语
WOS记录号WOS:000364232100095
项目编号81300851 ; SKLODd2009001
资助机构National Natural Science Foundation of China ; Beijing Municipal Natural Science Foundation ; Peking University School of Stamotology Youth Fund
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67639
专题北京大学口腔医学院_第二门诊部
北京大学医学部管理机构_医学部
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Dent Ctr 2, Sch & Hosp Stomatol, Beijing 100101, Peoples R China
2.Peking Univ, Natl Engn Lab Digital & Mat Technol Stomatol, Sch & Hosp Stomatol, Beijing 100081, Peoples R China
3.Peking Univ, Dept Lab Anim Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
4.Peking Univ, Dept Polymer Sci & Engn, Coll Chem & Mol Engn, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Hu, Hongcheng,Pu, Yinfei,Lu, Songhe,et al. The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2015,16(10):24946-24964.
APA Hu, Hongcheng.,Pu, Yinfei.,Lu, Songhe.,Zhang, Kuo.,Guo, Yuan.,...&Tang, Zhihui.(2015).The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,16(10),24946-24964.
MLA Hu, Hongcheng,et al."The Osteogenesis Effect and Underlying Mechanisms of Local Delivery of gAPN in Extraction Sockets of Beagle Dogs".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 16.10(2015):24946-24964.
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