IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
Mechanical characterization of cervical squamous carcinoma cells by atomic force microscopy at nanoscale
Ding, Yong-xia1; Cheng, Yuan1; Sun, Quan-mei2; Zhang, You-yi3,4; You, Ke1; Guo, Yan-li1; Han, Dong2; Geng, Li1
关键词Cervical cancer AFM Young&prime s modulus P16
刊名MEDICAL ONCOLOGY
2015-03-01
DOI10.1007/s12032-015-0507-0
32期:3
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
研究领域[WOS]Oncology
关键词[WOS]INTRAEPITHELIAL NEOPLASIA ; CANCER ; STIFFNESS ; WOMEN ; AFM
英文摘要

To investigate the nanoscale mechanical properties of exfoliated cervical epithelial cells from patients to further reveal the pathogenesis of cervical cancer and help early diagnose. Exfoliated cells were collected from nine patients with chronic cervicitis or CIN1(control group), 30 patients with CIN2-3 (CIN 2-3 group), and 13 patients with cervical cancer (cervical cancer group). Stiffness of the cells was determined by atomic force microscope (AFM). Expression of P16INK4A was studied by immunocytochemistry. Environmental scanning electron microscopy was performed to observe the surface microtopography of the exfoliated cells. Young′s modulus was measured for cells exfoliated from control and patients with CIN 2-3 and cervical cancer by AFM. The results showed that with increasing cervical lesions, the Young′s modulus of the exfoliated cervical cells increased (P < 0.05). The modulus of the exfoliated cells was significantly decreased in the three patients 1 year after the surgery compared with the value before the surgery. Expression of P16INK4A in the exfoliated cells had not been statistically significant. Squamous cells from cervical cancer group had dense and disordered microvilli without clear microridges compare to other groups. The Young′s modulus is increased from the control group, to CIN2-3 and cervical cancer groups, suggesting that the stiffness of cervical epithelial cells increases gradually with increasing cervical lesions. The changes in the mechanical properties of the exfoliated cells occur earlier than the changes in cell morphology. Therefore, analysis of mechanical properties of the exfoliated cells may be used to aid early diagnosis of the cancer.

语种英语
所属项目编号81472429 ; 2013CB933702
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program)
WOS记录号WOS:000350060000033
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67642
专题北京大学第三临床医学院
作者单位1.Peking Univ, Dept Gynecol & Obstet, Hosp 3, Beijing 100191, Peoples R China
2.Natl Ctr Nanosci & Technol, Lab Biol Imaging & Nanomed, Beijing 100190, Peoples R China
3.Peking Univ, Inst Vasc Med, Hosp 3, Beijing 100191, Peoples R China
4.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptide, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ding, Yong-xia,Cheng, Yuan,Sun, Quan-mei,et al. Mechanical characterization of cervical squamous carcinoma cells by atomic force microscopy at nanoscale[J]. MEDICAL ONCOLOGY,2015,32(3).
APA Ding, Yong-xia.,Cheng, Yuan.,Sun, Quan-mei.,Zhang, You-yi.,You, Ke.,...&Geng, Li.(2015).Mechanical characterization of cervical squamous carcinoma cells by atomic force microscopy at nanoscale.MEDICAL ONCOLOGY,32(3).
MLA Ding, Yong-xia,et al."Mechanical characterization of cervical squamous carcinoma cells by atomic force microscopy at nanoscale".MEDICAL ONCOLOGY 32.3(2015).
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