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学科主题: 临床医学
题名:
CRISPR/CAS9-Mediated Genome Editing of miRNA-155 Inhibits Proinflammatory Cytokine Production by RAW264.7 Cells
作者: Jing, Weixia1; Zhang, Xuewu2; Sun, Wenyan3; Hou, Xiujuan4; Yao, Zhongqiang5; Zhu, Yuelan4
刊名: BIOMED RESEARCH INTERNATIONAL
发表日期: 2015
DOI: 10.1155/2015/326042
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Medicine, Research & Experimental
研究领域[WOS]: Biotechnology & Applied Microbiology ; Research & Experimental Medicine
关键词[WOS]: INDUCED CYTIDINE DEAMINASE ; RHEUMATOID-ARTHRITIS ; MICRORNA-155 ; RNA ; CAS9 ; REGULATOR ; MIR-155 ; PATHWAY ; GENE
英文摘要:

MicroRNA 155 (miR-155) is a key proinflammatory regulator in clinical and experimental rheumatoid arthritis (RA). Here we generated a miR-155 genome knockout (GKO) RAW264.7 macrophage cell line using the clustered regulatory interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CAS9) technology. While upregulating the Src homology-2 domain-containing inositol 5-phosphatase 1 (SHIP1), the miR-155 GKO line is severely impaired in producing proinflammatory cytokines but slightly increased in osteoclastogenesis upon treatment with receptor activator of nuclear factor-kappa B ligand (RANKL). Taken together, our results suggest that genome editing of miR-155 holds the potential as a therapeutic strategy in RA.

语种: 英语
所属项目编号: 81273697
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000366410100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67643
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Beijing Univ Chinese Med, Affiliated Hosp 3, Dept Med Serv, Beijing 100029, Peoples R China
2.Peking Univ, Peoples Hosp, Clin Immunol Ctr, Dept Rheumatol & Immunol, Beijing 100044, Peoples R China
3.Beijing Univ Chinese Med, Sch Chinese Med, Dept Pharmacol, Beijing 100102, Peoples R China
4.Beijing Univ Chinese Med, Dongfang Hosp, Dept Rheumatol, Beijing 100078, Peoples R China
5.Peking Univ, Hosp 3, Dept Rheumatol & Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Jing, Weixia,Zhang, Xuewu,Sun, Wenyan,et al. CRISPR/CAS9-Mediated Genome Editing of miRNA-155 Inhibits Proinflammatory Cytokine Production by RAW264.7 Cells[J]. BIOMED RESEARCH INTERNATIONAL,2015.
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