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Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients
Liu, Caiyun1; Guo, Jianping1; Qu, Like1; Bing, Dong2; Meng, Lin1; Wu, Jian1; Shou, Chengchao1
关键词synuclein-gamma monoclonal antibody colorectal cancer immunoassay
刊名CANCER LETTERS
2008-09-28
DOI10.1016/j.canlet.2008.04.037
269期:1页:148-158
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]GENE CPG ISLAND ; BREAST-CANCER ; ALPHA-SYNUCLEIN ; PARKINSONS-DISEASE ; HUMAN PLASMA ; EXPRESSION ; CELLS ; LOCALIZATION ; PROMOTES ; IDENTIFICATION
英文摘要

Overexpressions of synuclein-gamma (SNCG) in different cancers display stage-specific patterns. At present, appropriate anti-SNCG monoclonal antibodies (mAbs) with high specificity and affinity are unavailable for different immunoassays in clinical applications. In this study, we generated 10 mAbs against endogenous SNCG and evaluated SNCG levels in several colorectal cancer cell lines, serum samples and tumor tissues from colorectal cancer (CRC) patients. Elevated SNCG levels in cancer cell lines evaluated by a novel sandwich ELISA were consistent with data obtained from Western blot. Secreted SNCG protein levels in sera from CRC patients could be detected by the sandwich ELISA and were further confirmed by Western blot analysis following SNCG enrichment. Immunohistochemical results showed that SNCG was highly expressed in tumor cells of CRC patients, but was undetectable in the adjacent normal epithelium. Taken together, these novel anti-SNCG mAbs specifically recognized endogenous SNCG and were suitable for measuring SNCG levels in cell lysates, human serum samples, and tumor tissues. Elevated serum SNCG and overexpressed SNCG in tumor tissue from CRC patients suggest SNCG is a potential biomarker for CRC. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000259847100017
项目编号20022023 ; 2005-3051 ; 2006AA02A249
资助机构Foundation of Capital Medicine Development of Beijing ; Peking university and 863 High Technology Program
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67651
专题北京大学临床肿瘤学院_病理科
北京大学第三临床医学院_检验科
北京大学临床肿瘤学院_生化与分子生物学研究室
作者单位1.Peking Univ, Key Lab Minist Educat Canc Res & Applicat, Dept Biochem & Mol Biol, Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China
2.Peking Univ, Dept Pathol, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China
推荐引用方式
GB/T 7714
Liu, Caiyun,Guo, Jianping,Qu, Like,et al. Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients[J]. CANCER LETTERS,2008,269(1):148-158.
APA Liu, Caiyun.,Guo, Jianping.,Qu, Like.,Bing, Dong.,Meng, Lin.,...&Shou, Chengchao.(2008).Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients.CANCER LETTERS,269(1),148-158.
MLA Liu, Caiyun,et al."Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients".CANCER LETTERS 269.1(2008):148-158.
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