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学科主题: 临床医学
题名:
Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients
作者: Liu, Caiyun1; Guo, Jianping1; Qu, Like1; Bing, Dong2; Meng, Lin1; Wu, Jian1; Shou, Chengchao1
关键词: synuclein-gamma ; monoclonal antibody ; colorectal cancer ; immunoassay
刊名: CANCER LETTERS
发表日期: 2008-09-28
DOI: 10.1016/j.canlet.2008.04.037
卷: 269, 期:1, 页:148-158
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: GENE CPG ISLAND ; BREAST-CANCER ; ALPHA-SYNUCLEIN ; PARKINSONS-DISEASE ; HUMAN PLASMA ; EXPRESSION ; CELLS ; LOCALIZATION ; PROMOTES ; IDENTIFICATION
英文摘要:

Overexpressions of synuclein-gamma (SNCG) in different cancers display stage-specific patterns. At present, appropriate anti-SNCG monoclonal antibodies (mAbs) with high specificity and affinity are unavailable for different immunoassays in clinical applications. In this study, we generated 10 mAbs against endogenous SNCG and evaluated SNCG levels in several colorectal cancer cell lines, serum samples and tumor tissues from colorectal cancer (CRC) patients. Elevated SNCG levels in cancer cell lines evaluated by a novel sandwich ELISA were consistent with data obtained from Western blot. Secreted SNCG protein levels in sera from CRC patients could be detected by the sandwich ELISA and were further confirmed by Western blot analysis following SNCG enrichment. Immunohistochemical results showed that SNCG was highly expressed in tumor cells of CRC patients, but was undetectable in the adjacent normal epithelium. Taken together, these novel anti-SNCG mAbs specifically recognized endogenous SNCG and were suitable for measuring SNCG levels in cell lysates, human serum samples, and tumor tissues. Elevated serum SNCG and overexpressed SNCG in tumor tissue from CRC patients suggest SNCG is a potential biomarker for CRC. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
所属项目编号: 20022023 ; 2005-3051 ; 2006AA02A249
项目资助者: Foundation of Capital Medicine Development of Beijing ; Peking university and 863 High Technology Program
WOS记录号: WOS:000259847100017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67651
Appears in Collections:北京大学临床肿瘤学院_病理科_期刊论文

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作者单位: 1.Peking Univ, Key Lab Minist Educat Canc Res & Applicat, Dept Biochem & Mol Biol, Sch Oncol,Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China
2.Peking Univ, Dept Pathol, Sch Oncol, Beijing Canc Hosp & Inst, Beijing 100036, Peoples R China

Recommended Citation:
Liu, Caiyun,Guo, Jianping,Qu, Like,et al. Applications of novel monoclonal antibodies specific for synuclein-gamma in evaluating its levels in sera and cancer tissues from colorectal cancer patients[J]. CANCER LETTERS,2008,269(1):148-158.
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