IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Inhibitory effects of tetrandrine on the serum- and platelet-derived growth factor-BB-induced proliferation of rat aortic smooth muscle cells through inhibition of cell cycle progression, DNA synthesis, ERK1/2 activation and c-fos expression
Fang, LH; Zhang, YH; Ma, JJ; Du, GH; Ku, BS; Yao, HY; Yun, YP; Kim, TJ
关键词tetrandrine proliferation cell cycle MAPK c-fos
刊名ATHEROSCLEROSIS
2004-06-01
DOI10.1016/j.atherosclerosis.2004.01.036
174期:2页:215-223
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]PROTEIN-KINASE ; CA2+ TRANSIENTS ; PRIMARY CULTURE ; ATHEROSCLEROSIS ; MIGRATION ; MECHANISMS ; PATHWAY
英文摘要

Tetrandrine (TET) is a well known naturally occurred nonspecific Ca2+ channel blocker. It has long been used for the treatment of arrhythmia, hypertension, and occlusive cardiovascular disorders. The objective of the present study was to investigate the effect of TET on the proliferation of primary cultured rat aortic smooth muscle cells (RASMCs). TET significantly inhibited both 10% fetal bovine serum (FBS) and 50 ng/ml platelet-derived growth factor (PDGF)-BB-induced proliferation, [H-3]thymidine incorporation into DNA, and p42/p44 mitogen-activated protein kinase (ERK1/2) phosphorylation at the concentration of 1.0 and 5.0 muM. Flow cytometry analysis of DNA content in synchronized cells revealed blocking of the FBS-inducible cell cycle progression by TET. In accordance with these findings, TET 5 muM caused a 48% decrease in the early elevation of c-fos expression induced after 10% FBS addition. Furthermore, in contrast to its distinguishable higher potency of Ca2+ antagonistic activity, verapamil showed lower potent antiproliferative activities than TET. These results suggest that TET can exert antiproliferative effects against mitogenic stimuli for RASMCs in vitro by a mechanism that involves the MAPK pathway, altering cell cycle progression, and the inhibitory action cannot be limited to its Ca2+ modulation. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000221674200003
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67655
专题北京大学基础医学院_药理学系
作者单位1.Yanbian Univ, Coll Pharm, Yanji 133000, Peoples R China
2.Chungbuk Natl Univ, Coll Pharmaceut, Cheongju, South Korea
3.Peking Univ, Dept Pharmacol, Sch Basic Med Sci, Beijing 100083, Peoples R China
4.Chinese Acad Med Sci, Inst Mat Med, Natl Ctr Pharmaceut Screening, Beijing 100050, Peoples R China
5.Peking Union Med Coll, Beijing 100050, Peoples R China
推荐引用方式
GB/T 7714
Fang, LH,Zhang, YH,Ma, JJ,et al. Inhibitory effects of tetrandrine on the serum- and platelet-derived growth factor-BB-induced proliferation of rat aortic smooth muscle cells through inhibition of cell cycle progression, DNA synthesis, ERK1/2 activation and c-fos expression[J]. ATHEROSCLEROSIS,2004,174(2):215-223.
APA Fang, LH.,Zhang, YH.,Ma, JJ.,Du, GH.,Ku, BS.,...&Kim, TJ.(2004).Inhibitory effects of tetrandrine on the serum- and platelet-derived growth factor-BB-induced proliferation of rat aortic smooth muscle cells through inhibition of cell cycle progression, DNA synthesis, ERK1/2 activation and c-fos expression.ATHEROSCLEROSIS,174(2),215-223.
MLA Fang, LH,et al."Inhibitory effects of tetrandrine on the serum- and platelet-derived growth factor-BB-induced proliferation of rat aortic smooth muscle cells through inhibition of cell cycle progression, DNA synthesis, ERK1/2 activation and c-fos expression".ATHEROSCLEROSIS 174.2(2004):215-223.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Fang, LH]的文章
[Zhang, YH]的文章
[Ma, JJ]的文章
百度学术
百度学术中相似的文章
[Fang, LH]的文章
[Zhang, YH]的文章
[Ma, JJ]的文章
必应学术
必应学术中相似的文章
[Fang, LH]的文章
[Zhang, YH]的文章
[Ma, JJ]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。