IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
PKC delta mediates thrombin-augmented fibroblast-mediated collagen gel contraction
Fang, Qiuhong2; Mao, Lijun3; Kobayashi, Tetsu4; Wang, Xingqi1; Wyatt, Todd A.1; Kim, Huijung5; Liu, Xiangde1; Rennard, Stephen I.1
关键词fibroblast PKC tissue repair
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2008-05-16
DOI10.1016/j.bbrc.2008.03.016
369期:4页:1199-1203
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]PROTEIN-KINASE-C ; BRONCHIAL EPITHELIAL-CELLS ; ACTIVATED RECEPTORS ; INACTIVATION ; ALPHA ; PHOSPHORYLATION ; DIACYLGLYCEROL ; INFLAMMATION ; MECHANISMS ; ISOFORMS
英文摘要

Fibroblast-mediated collagen gel contraction has been used as an in vitro model of tissue remodeling. Thrombin is one of the mediators present in the milieu of airway inflammation and may be involved in airway tissue remodeling. We have previously reported that thrombin stimulates fibroblast-mediated collagen gel contraction partially through the PAR1/PKC epsilon signaling pathway [Q. Fang, X. Liu, S. Abe, T. Kobayashi, X.Q. Wang, T. Kohyama, M. Hashimoto, T. Wyatt, S.I. Rennard, Thrombin induces collagen gel contraction partially through PAR] activation and PKC-epsilon, Eur. Respir. J. 24 (2004) 918-924]. Here, we further report that the delta-isoform of PKC (PKC delta) is also activated by thrombin and involved in the thrombin-mediated augmentation of collagen gel contraction. Thrombin (10nM) significantly increased PKC delta activity (over 5-fold increase after 15-30 min stimulation) and stimulated phosphorylation of PKC delta. Rottlerin, a PKC delta inhibitor, Completely inhibited activation of PKC delta and partially blocked collagen gel contraction stimulated by thrombin. Similarly, PKC delta-specific siRNA significantly inhibited PKC delta activation without affecting PKC delta expression and activation. Furthermore, suppression of PKC delta by siRNA resulted in partial blockade of thrombin-augmented collagen gel contraction. These results suggest that thrombin contributes to the tissue remodeling in inflammatory airways and lung diseases at least partially through both PKC delta and PKCE signaling. (C) 2008 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000254864700041
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67656
专题北京大学第三临床医学院
作者单位1.Mie Univ, Grad Sch Med, Tsu, Mie 514, Japan
2.Wonkwang Univ, Kunpo Med Ctr, Seoul, South Korea
3.Peking Univ, Hosp 3, Beijing, Peoples R China
4.Univ Nebraska Med Ctr, Omaha, NE 68198 USA
5.Peking Univ, Beijing Shijitan Hosp, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Fang, Qiuhong,Mao, Lijun,Kobayashi, Tetsu,et al. PKC delta mediates thrombin-augmented fibroblast-mediated collagen gel contraction[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,369(4):1199-1203.
APA Fang, Qiuhong.,Mao, Lijun.,Kobayashi, Tetsu.,Wang, Xingqi.,Wyatt, Todd A..,...&Rennard, Stephen I..(2008).PKC delta mediates thrombin-augmented fibroblast-mediated collagen gel contraction.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,369(4),1199-1203.
MLA Fang, Qiuhong,et al."PKC delta mediates thrombin-augmented fibroblast-mediated collagen gel contraction".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 369.4(2008):1199-1203.
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