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学科主题: 临床医学
题名:
PKC delta mediates thrombin-augmented fibroblast-mediated collagen gel contraction
作者: Fang, Qiuhong2; Mao, Lijun3; Kobayashi, Tetsu4; Wang, Xingqi1; Wyatt, Todd A.1; Kim, Huijung5; Liu, Xiangde1; Rennard, Stephen I.1
关键词: fibroblast ; PKC ; tissue repair
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2008-05-16
DOI: 10.1016/j.bbrc.2008.03.016
卷: 369, 期:4, 页:1199-1203
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PROTEIN-KINASE-C ; BRONCHIAL EPITHELIAL-CELLS ; ACTIVATED RECEPTORS ; INACTIVATION ; ALPHA ; PHOSPHORYLATION ; DIACYLGLYCEROL ; INFLAMMATION ; MECHANISMS ; ISOFORMS
英文摘要:

Fibroblast-mediated collagen gel contraction has been used as an in vitro model of tissue remodeling. Thrombin is one of the mediators present in the milieu of airway inflammation and may be involved in airway tissue remodeling. We have previously reported that thrombin stimulates fibroblast-mediated collagen gel contraction partially through the PAR1/PKC epsilon signaling pathway [Q. Fang, X. Liu, S. Abe, T. Kobayashi, X.Q. Wang, T. Kohyama, M. Hashimoto, T. Wyatt, S.I. Rennard, Thrombin induces collagen gel contraction partially through PAR] activation and PKC-epsilon, Eur. Respir. J. 24 (2004) 918-924]. Here, we further report that the delta-isoform of PKC (PKC delta) is also activated by thrombin and involved in the thrombin-mediated augmentation of collagen gel contraction. Thrombin (10nM) significantly increased PKC delta activity (over 5-fold increase after 15-30 min stimulation) and stimulated phosphorylation of PKC delta. Rottlerin, a PKC delta inhibitor, Completely inhibited activation of PKC delta and partially blocked collagen gel contraction stimulated by thrombin. Similarly, PKC delta-specific siRNA significantly inhibited PKC delta activation without affecting PKC delta expression and activation. Furthermore, suppression of PKC delta by siRNA resulted in partial blockade of thrombin-augmented collagen gel contraction. These results suggest that thrombin contributes to the tissue remodeling in inflammatory airways and lung diseases at least partially through both PKC delta and PKCE signaling. (C) 2008 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000254864700041
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67656
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Mie Univ, Grad Sch Med, Tsu, Mie 514, Japan
2.Wonkwang Univ, Kunpo Med Ctr, Seoul, South Korea
3.Peking Univ, Hosp 3, Beijing, Peoples R China
4.Univ Nebraska Med Ctr, Omaha, NE 68198 USA
5.Peking Univ, Beijing Shijitan Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Fang, Qiuhong,Mao, Lijun,Kobayashi, Tetsu,et al. PKC delta mediates thrombin-augmented fibroblast-mediated collagen gel contraction[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,369(4):1199-1203.
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