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High-performance liquid chromatographic determination of tanshinones in the roots of Salvia miltiorrhiza and related traditional Chinese medicinal preparations
Liu, AH; Lin, YH; Yang, M; Sun, JH; Guo, H; Guo, DA
刊名JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
2006
9期:1页:1-9
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]NATURAL-PRODUCTS ; CELLS ; PROLIFERATION ; EXTRACTION ; BUNGE
英文摘要

Purpose: This paper describes a validated high-performance liquid chromatographic method to quantitate four tanshinones as markers; dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA for use in the quality control of the roots of Salvia miltiorrhiza and its related traditional Chinese medicinal preparations. Methods: Separation was achieved using a Zorbax Extend C18 reserved-phase column (5 mu m, 250 x 4.6mm) at 20 degrees C with a gradient mixture of deionized water and acetonitrile at a flow rate of 1.2ml/ min. Result: The limits of quantitation were 0.13, 0.08, 0.06 and 0.05 mu g/ml for dihydrotanshinone I, cryptotanshinone, tanshinone I and tanshinone IIA, respectively. This method provided good reproducibility and sensitivity for the quantification of four tanshinones with overall RSD values for intra-day and inter-day precision and accuracy better than 3.8% and higher than 94.9%, respectively. The recovery of the method was 95.4- 104.4% for all the tanshinones and showed good linearity ( r> 0.9998) over a relatively wide concentration range. Conclusions: This assay was successfully applied to the determination of four tanshinones in the roots of Salvia miltiorrhiza and its related traditional Chinese medicinal preparations. The results indicated that the HPLC assay could be readily utilized as a quality control method for the roots of Salvia miltiorrhiza and its related traditional Chinese medicinal preparations.

语种英语
WOS记录号WOS:000236885300001
Citation statistics
Cited Times:44[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67659
Collection北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Liu, AH,Lin, YH,Yang, M,et al. High-performance liquid chromatographic determination of tanshinones in the roots of Salvia miltiorrhiza and related traditional Chinese medicinal preparations[J]. JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES,2006,9(1):1-9.
APA Liu, AH,Lin, YH,Yang, M,Sun, JH,Guo, H,&Guo, DA.(2006).High-performance liquid chromatographic determination of tanshinones in the roots of Salvia miltiorrhiza and related traditional Chinese medicinal preparations.JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES,9(1),1-9.
MLA Liu, AH,et al."High-performance liquid chromatographic determination of tanshinones in the roots of Salvia miltiorrhiza and related traditional Chinese medicinal preparations".JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES 9.1(2006):1-9.
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