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学科主题: 临床医学
题名:
Treatment of Mice with Dextran Sulfate Sodium-Induced Colitis with Human Interleukin 10 Secreted by Transformed Bifidobacterium longum
作者: Yao, Jun1; Wang, Jian-yao3; Lai, Ming-Guang1; Li, Ying-xue1; Zhu, Hui-ming1; Shi, Rui-yue1; Mo, Jing4; Xun, An-ying5; Jia, Chun-hong2; Feng, Ju-ling1; Wang, Li-Sheng1; Zeng, Wei-sen2; Liu, Lei3
关键词: ulcerative colitis ; Bifidobacterium longum ; BL-hIL-10 ; interleukin 10
刊名: MOLECULAR PHARMACEUTICS
发表日期: 2011-03-01
DOI: 10.1021/mp100331r
卷: 8, 期:2, 页:488-497
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: INFLAMMATORY-BOWEL-DISEASE ; REGULATORY T-CELLS ; ULCERATIVE-COLITIS ; MURINE COLITIS ; IMMUNE-RESPONSES ; BACTERIAL ; IL-10 ; INHIBITION ; PREVENTION ; FOXP3
英文摘要:

Ulcerative colitis (UC) is an inflammatory bowel disease (IBD) the etiology of which has not yet been fully clarified. Cytokine interleukin-10 (IL-10) plays a central role in downregulating inflammatory cascade in UC and is likely a candidate for therapeutic intervention. However, its intravenous administration is costly and inconvenient. Therefore, we established a novel IL-10 delivery system by transforming a hIL-10-containing plasmid into B. longum (BL-hIL-10) and investigated its effects on 5% dextran sulfate sodium (DSS)-induced ulcerative colitis in mice and the possible underlying mechanism. Our results show that (1) hIL-10 was expressed and secreted into the culture supernatant of BL-hIL-10 after L-arabinose induction in vitro as examined by Western blot, enzyme-linked immunosorbent assay (ELISA) and RT-PCR; (2) addition of BL-hIL-10 culture supernatant had no cytotoxic effect and morphological alteration, but significantly inhibited the enhancement of proinflammatory cytokines by lipopolysaccharide (LPS) in THP-1 cells; (3) oral administration of BL-hIL-10 alleviated colitis syndrome of the model mice, attenuated colitis-activated NF-kappa B pathway measured by DNA-binding assay and colitis-elevated expression of proinflammatory cytokines examined with CCK cytotoxic kits, and upregulated CD4(+)CD25(+)Foxp3(+) Treg in blood and mesenteric lymph nodes measured by flow cytometry. In conclusion, BL-hIL-10 as a novel oral hIL-10 delivery system has been successfully established and oral administration of BL-hIL-10 alleviated inflammatory damage of colonic tissue in the model mice by blocking the colitis-activated NF-kappa B pathway and upregulating CD4(+)CD25(+)Foxp3(+) Treg in blood and mesenteric lymph nodes in mice.

语种: 英语
WOS记录号: WOS:000289008600018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67663
Appears in Collections:北京大学深圳医院_消化内科_期刊论文

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作者单位: 1.Jinan Univ Med Sci, Shenzhen Municipal Peoples Hosp, Dept Gastroenterol, Shenzhen 518020, Guangdong, Peoples R China
2.So Med Univ, Dept Cell Biol, Guangzhou 510515, Guangdong, Peoples R China
3.Shenzhen Childrens Hosp, Dept Gen Surg, Shenzhen 518026, Guangdong, Peoples R China
4.Guangzhou Med Univ, Affiliated Hosp 3, Dept Pediat, Guangzhou 510150, Guangdong, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Gastroenterol, Shenzhen 518020, Guangdong, Peoples R China

Recommended Citation:
Yao, Jun,Wang, Jian-yao,Lai, Ming-Guang,et al. Treatment of Mice with Dextran Sulfate Sodium-Induced Colitis with Human Interleukin 10 Secreted by Transformed Bifidobacterium longum[J]. MOLECULAR PHARMACEUTICS,2011,8(2):488-497.
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