IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Multiple genetic switches spontaneously modulating bacterial mutability
Chen, Fang1,2; Liu, Wei-Qiao1,3; Eisenstark, Abraham4,5; Johnston, Randal N.6; Liu, Gui-Rong1; Liu, Shu-Lin1,2,3
刊名BMC EVOLUTIONARY BIOLOGY
2010-09-13
DOI10.1186/1471-2148-10-277
10期:1
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Evolutionary Biology ; Genetics & Heredity
研究领域[WOS]Evolutionary Biology ; Genetics & Heredity
关键词[WOS]DNA MISMATCH REPAIR ; ESCHERICHIA-COLI POPULATIONS ; STRESS-INDUCED MUTAGENESIS ; HIGH MUTATION-RATES ; SALMONELLA-TYPHIMURIUM ; ADAPTIVE EVOLUTION ; HIGH-FREQUENCY ; GENOME ; MUTL ; MECHANISMS
英文摘要

Background: All life forms need both high genetic stability to survive as species and a degree of mutability to evolve for adaptation, but little is known about how the organisms balance the two seemingly conflicting aspects of life: genetic stability and mutability. The DNA mismatch repair (MMR) system is essential for maintaining genetic stability and defects in MMR lead to high mutability. Evolution is driven by genetic novelty, such as point mutation and lateral gene transfer, both of which require genetic mutability. However, normally a functional MMR system would strongly inhibit such genomic changes. Our previous work indicated that MMR gene allele conversion between functional and non-functional states through copy number changes of small tandem repeats could occur spontaneously via slipped-strand mis-pairing during DNA replication and therefore may play a role of genetic switches to modulate the bacterial mutability at the population level. The open question was: when the conversion from functional to defective MMR is prohibited, will bacteria still be able to evolve by accepting laterally transferred DNA or accumulating mutations?

Results: To prohibit allele conversion, we "locked" the MMR genes through nucleotide replacements. We then scored changes in bacterial mutability and found that Salmonella strains with MMR locked at the functional state had significantly decreased mutability. To determine the generalizability of this kind of mutability ′switching′ among a wider range of bacteria, we examined the distribution of tandem repeats within MMR genes in over 100 bacterial species and found that multiple genetic switches might exist in these bacteria and may spontaneously modulate bacterial mutability during evolution.

Conclusions: MMR allele conversion through repeats-mediated slipped-strand mis-pairing may function as a spontaneous mechanism to switch between high genetic stability and mutability during bacterial evolution.

语种英语
WOS记录号WOS:000282769600002
项目编号NSFC30970078 ; NSFC30870098 ; 30970119 ; 81030029 ; 20092307110001
资助机构Canadian Institutes of Health Research ; National Natural Science Foundation of China ; Natural Science Foundation of Heilongjiang Province of China ; Harbin Medical University ; Peking University Health Science Center ; Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP)
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67673
专题北京大学基础医学院_病原生物学系
作者单位1.Canc Res Ctr, Columbia, MO USA
2.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
3.Univ Missouri, Columbia, MO USA
4.Univ Calgary, Dept Biochem & Mol Biol, Calgary, AB, Canada
5.Harbin Med Coll, Genom Res Ctr, State Prov Key Labs Biomed Pharmaceut China, Harbin, Peoples R China
6.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Chen, Fang,Liu, Wei-Qiao,Eisenstark, Abraham,et al. Multiple genetic switches spontaneously modulating bacterial mutability[J]. BMC EVOLUTIONARY BIOLOGY,2010,10(1).
APA Chen, Fang,Liu, Wei-Qiao,Eisenstark, Abraham,Johnston, Randal N.,Liu, Gui-Rong,&Liu, Shu-Lin.(2010).Multiple genetic switches spontaneously modulating bacterial mutability.BMC EVOLUTIONARY BIOLOGY,10(1).
MLA Chen, Fang,et al."Multiple genetic switches spontaneously modulating bacterial mutability".BMC EVOLUTIONARY BIOLOGY 10.1(2010).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Multiple genetic swi(2426KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Chen, Fang]的文章
[Liu, Wei-Qiao]的文章
[Eisenstark, Abraham]的文章
百度学术
百度学术中相似的文章
[Chen, Fang]的文章
[Liu, Wei-Qiao]的文章
[Eisenstark, Abraham]的文章
必应学术
必应学术中相似的文章
[Chen, Fang]的文章
[Liu, Wei-Qiao]的文章
[Eisenstark, Abraham]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Multiple genetic switches spontaneously modulating bacterial mutability.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。