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Genetic environment of beta-lactamase genes of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates from patients with lower respiratory tract infection in China
Liu Lin1; Wang Xiaorong2; An Shuchang3; Zhang Xiangyan4; Chen Lin1; Li Yuqian1; Xu Li1; Zhang Yijie1; Gao Zhancheng1
关键词Klebsiella pneumoniae promoter beta-lactamase insertion sequence
刊名CHINESE MEDICAL JOURNAL
2014-07-05
DOI10.3760/cma.j.issn.0366-6999.20133307
127期:13页:2445-2450
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]ESCHERICHIA-COLI ; KLUYVERA-ASCORBATA ; BLA(CTX-M) GENES ; RESISTANCE ; ENTEROBACTERIACEAE ; PREVALENCE ; EXPRESSION ; PROMOTERS ; PLASMIDS ; CTX-M-3
英文摘要

Background Extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae (K. pneumoniae) is one of the most popular pathogens that cause refractory respiratory tract infection. The genetic environment, including insertion sequences and the types of promoter, plays a key role in exploration of the mechanism of prevalence and dismission of the ESBL-producing K. pneumoniae isolates. The aim of the investigation was to target analysis the genetic environment and promoter sequences of bla(CTX-M), bla(SHV) and bla(TEM), the most popular beta-lactamase genes harbored by ESBL-producing K. pneumoniae isolates.

Methods From February 2010 to July 2011, 158 of 416 K. pneumoniae isolates producing ESBL from patients with lower respiratory tract infection were collected from seven tertiary hospitals from Beijing, Anhui, Fujian, Liaoning, Hebei and Inner Mongolia Autonomous Region in China. The genetic environment including promoters of 10 types of bla(CTX-M), 18 types of bla(SHV) and 2 types of bla(TEM) were analyzed by amplification and direct sequencing with various sets of PCR primers.

Results ISEcp1 was located upstream of the 5′ end of the bla(CTX-M) gene in 130 (97.0%) out of 134 K. pneumoniae isolates harboring bla(CTX-M) and provided a conserved promoter to bla(CTX-M). A non-coding sequence preceded by kdpC and recF was identified in all of the bla(SHV) genes except bla(SHV-12) and bla(SHV-2a). IS26 was also found upstream of 1 bla(CTX-M-15), 10 bla(SHV-1), strains, 4 bla(TEM-1) and all of the bla(SHV-2), bla(SHV-2a), bla(SHV-5) and bla(SHV-12). Eighty-seven of 91 strains harboring bla(TEM-1) carried a copy of Tn2 and IS26-bla(TEM-1), fragments were also detected in 4 strains. With respect to K. pneumoniae, the genetic environment of bla(CTX-M-38), bla(SHV-142) and bla(TEM-135) were firstly elaborated, and four kinds of novel genetic environment of bla(CTX-M-3), bia(CTX-M-15) and bla(TEM-1) have been detected as well.

Conclusions Perfective implementation of the genetic environment information of beta-lactamase gene needs to be further explored and supplemented. ISEcp1 and IS26 elements are widespread upstream of the bla(CTX-M), bla(SHV) and bla(TEM) genes and contribute to horizontal transmission and genetic expression.

语种英语
WOS记录号WOS:000339470600009
项目编号2006AA02Z4A9 ; 2012BAI05B02 ; 2012ZX10004-206 ; 201202011
资助机构National High-Tech R&amp ; D Program of the Ministry of Science and Technology of China ; National 12th Five-Year Plan Major Scientific and Technological Program of the Ministry of Science and Technology of China ; Special Fund for Health-scientific Research in the Public Interest Program of the Ministry of Health of China
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67677
专题北京大学第二临床医学院
医学人文研究院/公共教学部_哲学与社会科学系
北京大学第二临床医学院_呼吸科
作者单位1.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
2.Huazhong Univ Sci & Technol, Dept Resp Med, Affiliated Union Hosp, Wuhan 430022, Hubei, Peoples R China
3.Tsinghua Univ, Affiliated Hosp 1, Dept Resp Med, Beijing 100016, Peoples R China
4.Guizhou Prov Peoples Hosp, Dept Resp & Crit Care Med, Guiyang 550002, Guizhou, Peoples R China
推荐引用方式
GB/T 7714
Liu Lin,Wang Xiaorong,An Shuchang,et al. Genetic environment of beta-lactamase genes of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates from patients with lower respiratory tract infection in China[J]. CHINESE MEDICAL JOURNAL,2014,127(13):2445-2450.
APA Liu Lin.,Wang Xiaorong.,An Shuchang.,Zhang Xiangyan.,Chen Lin.,...&Gao Zhancheng.(2014).Genetic environment of beta-lactamase genes of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates from patients with lower respiratory tract infection in China.CHINESE MEDICAL JOURNAL,127(13),2445-2450.
MLA Liu Lin,et al."Genetic environment of beta-lactamase genes of extended-spectrum beta-lactamase-producing Klebsiella pneumoniae isolates from patients with lower respiratory tract infection in China".CHINESE MEDICAL JOURNAL 127.13(2014):2445-2450.
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