|Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer|
|Liu, Xue-en1,2,3; Dewaele, Sylviane1,3; Vanhooren, Valerie1,3; Fan, Ye-Dong4; Wang, Ling2; Van Huysse, Jacques5; Zhuang, Hui2; Contreras, Roland1,3; Libert, Claude1,3; Chen, Cuiying Chitty1,3|
|关键词||DENA hepatocellular carcinoma (HCC) marker N-glycan|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology|
|研究领域[WOS]||Gastroenterology & Hepatology|
|关键词[WOS]||SERUM-PROTEIN GLYCOMICS ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; MOUSE MODELS ; GLYCOSYLATION ; DNA ; RATS ; EXPRESSION ; HEPATOMA ; GLYCANS ; DISEASE|
Background and aims
There is a demand for serum markers that can routinely assess the progression of liver cancer. DENA (diethylnitrosamine), a hepatocarcinogen, is commonly used in an experimental mouse model to induce liver cancer that closely mimics a subclass of human hepatocellular carcinoma (HCC). However, blood monitoring of the progression of HCC in mouse model has not yet been achieved. In this report, we studied glycomics during the development of mouse HCC induced by DENA.
Mouse HCC was induced by DENA. Serum N-glycans were profiled using the sequencer assisted-Fluorophore-assisted carbohydrate electrophoresis technique developed in our laboratory. Possible alteration in the transcription of genes relevant to the synthesis of the changed glycans was analysed by real-time polymerase chain reaction.
In comparison with the control mice that received the same volume of saline, a tri-antennary glycan (peak 8) and a biantennary glycan (peak 4) in serum total glycans of DENA mice increased gradually but significantly during progression of liver cancer, whereas a core-fucosylated biantennary glycan (peak 6) decreased. Expression of alpha-1,6-fucosyltransferase 8 (Fut8), which is responsible for core fucosylation, decreased in the liver of DENA mice compared with that of age-matched control mice. Likewise, the expression level of Mgat4a, which is responsible for tri-antennary, significantly increased in the liver of DENA mice (P < 0.001).
The changes of N-glycan levels in the serum could be used as a biomarker to monitor the progress of HCC and to follow up the treatment of liver tumours in this DENA mouse model.
|项目编号||01106205 ; 011S605|
|资助机构||Ghent University (BOF) ; Flanders-China Bilateral project|
|作者单位||1.Univ Ghent, Dept Biomed Mol Biol, B-9052 Ghent, Belgium|
2.Ghent Univ Hosp, Dept Surg, B-9000 Ghent, Belgium
3.Ghent Univ Hosp, Dept Pathol, B-9000 Ghent, Belgium
4.Univ Ghent, VIB, Dept Mol Biomed Res, B-9052 Ghent, Belgium
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
|Liu, Xue-en,Dewaele, Sylviane,Vanhooren, Valerie,et al. Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer[J]. LIVER INTERNATIONAL,2010,30(8):1221-1228.|
|APA||Liu, Xue-en.,Dewaele, Sylviane.,Vanhooren, Valerie.,Fan, Ye-Dong.,Wang, Ling.,...&Chen, Cuiying Chitty.(2010).Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer.LIVER INTERNATIONAL,30(8),1221-1228.|
|MLA||Liu, Xue-en,et al."Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer".LIVER INTERNATIONAL 30.8(2010):1221-1228.|
|Alteration of N-glyc（1602KB）||期刊论文||出版稿||开放获取||CC BY-NC-SA||浏览 请求全文|