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学科主题: 基础医学
题名:
Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer
作者: Liu, Xue-en1,2,3; Dewaele, Sylviane1,3; Vanhooren, Valerie1,3; Fan, Ye-Dong4; Wang, Ling2; Van Huysse, Jacques5; Zhuang, Hui2; Contreras, Roland1,3; Libert, Claude1,3; Chen, Cuiying Chitty1,3
关键词: DENA ; hepatocellular carcinoma (HCC) ; marker ; N-glycan
刊名: LIVER INTERNATIONAL
发表日期: 2010-09-01
DOI: 10.1111/j.1478-3231.2010.02279.x
卷: 30, 期:8, 页:1221-1228
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: SERUM-PROTEIN GLYCOMICS ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; MOUSE MODELS ; GLYCOSYLATION ; DNA ; RATS ; EXPRESSION ; HEPATOMA ; GLYCANS ; DISEASE
英文摘要:

Background and aims

There is a demand for serum markers that can routinely assess the progression of liver cancer. DENA (diethylnitrosamine), a hepatocarcinogen, is commonly used in an experimental mouse model to induce liver cancer that closely mimics a subclass of human hepatocellular carcinoma (HCC). However, blood monitoring of the progression of HCC in mouse model has not yet been achieved. In this report, we studied glycomics during the development of mouse HCC induced by DENA.

Methods

Mouse HCC was induced by DENA. Serum N-glycans were profiled using the sequencer assisted-Fluorophore-assisted carbohydrate electrophoresis technique developed in our laboratory. Possible alteration in the transcription of genes relevant to the synthesis of the changed glycans was analysed by real-time polymerase chain reaction.

Results

In comparison with the control mice that received the same volume of saline, a tri-antennary glycan (peak 8) and a biantennary glycan (peak 4) in serum total glycans of DENA mice increased gradually but significantly during progression of liver cancer, whereas a core-fucosylated biantennary glycan (peak 6) decreased. Expression of alpha-1,6-fucosyltransferase 8 (Fut8), which is responsible for core fucosylation, decreased in the liver of DENA mice compared with that of age-matched control mice. Likewise, the expression level of Mgat4a, which is responsible for tri-antennary, significantly increased in the liver of DENA mice (P < 0.001).

Conclusions

The changes of N-glycan levels in the serum could be used as a biomarker to monitor the progress of HCC and to follow up the treatment of liver tumours in this DENA mouse model.

语种: 英语
所属项目编号: 01106205 ; 011S605
项目资助者: Ghent University (BOF) ; Flanders-China Bilateral project
WOS记录号: WOS:000280666800020
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67679
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ Ghent, Dept Biomed Mol Biol, B-9052 Ghent, Belgium
2.Ghent Univ Hosp, Dept Surg, B-9000 Ghent, Belgium
3.Ghent Univ Hosp, Dept Pathol, B-9000 Ghent, Belgium
4.Univ Ghent, VIB, Dept Mol Biomed Res, B-9052 Ghent, Belgium
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Xue-en,Dewaele, Sylviane,Vanhooren, Valerie,et al. Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer[J]. LIVER INTERNATIONAL,2010,30(8):1221-1228.
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