IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer
Liu, Xue-en1,2,3; Dewaele, Sylviane1,3; Vanhooren, Valerie1,3; Fan, Ye-Dong4; Wang, Ling2; Van Huysse, Jacques5; Zhuang, Hui2; Contreras, Roland1,3; Libert, Claude1,3; Chen, Cuiying Chitty1,3
关键词DENA hepatocellular carcinoma (HCC) marker N-glycan
刊名LIVER INTERNATIONAL
2010-09-01
DOI10.1111/j.1478-3231.2010.02279.x
30期:8页:1221-1228
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
资助者Ghent University (BOF) ; Flanders-China Bilateral project ; Ghent University (BOF) ; Flanders-China Bilateral project
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]SERUM-PROTEIN GLYCOMICS ; ACETYLGLUCOSAMINYLTRANSFERASE-III ; MOUSE MODELS ; GLYCOSYLATION ; DNA ; RATS ; EXPRESSION ; HEPATOMA ; GLYCANS ; DISEASE
英文摘要

Background and aims

There is a demand for serum markers that can routinely assess the progression of liver cancer. DENA (diethylnitrosamine), a hepatocarcinogen, is commonly used in an experimental mouse model to induce liver cancer that closely mimics a subclass of human hepatocellular carcinoma (HCC). However, blood monitoring of the progression of HCC in mouse model has not yet been achieved. In this report, we studied glycomics during the development of mouse HCC induced by DENA.

Methods

Mouse HCC was induced by DENA. Serum N-glycans were profiled using the sequencer assisted-Fluorophore-assisted carbohydrate electrophoresis technique developed in our laboratory. Possible alteration in the transcription of genes relevant to the synthesis of the changed glycans was analysed by real-time polymerase chain reaction.

Results

In comparison with the control mice that received the same volume of saline, a tri-antennary glycan (peak 8) and a biantennary glycan (peak 4) in serum total glycans of DENA mice increased gradually but significantly during progression of liver cancer, whereas a core-fucosylated biantennary glycan (peak 6) decreased. Expression of alpha-1,6-fucosyltransferase 8 (Fut8), which is responsible for core fucosylation, decreased in the liver of DENA mice compared with that of age-matched control mice. Likewise, the expression level of Mgat4a, which is responsible for tri-antennary, significantly increased in the liver of DENA mice (P < 0.001).

Conclusions

The changes of N-glycan levels in the serum could be used as a biomarker to monitor the progress of HCC and to follow up the treatment of liver tumours in this DENA mouse model.

语种英语
所属项目编号01106205 ; 011S605
资助者Ghent University (BOF) ; Flanders-China Bilateral project ; Ghent University (BOF) ; Flanders-China Bilateral project
WOS记录号WOS:000280666800020
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67679
专题北京大学基础医学院_病原生物学系
作者单位1.Univ Ghent, Dept Biomed Mol Biol, B-9052 Ghent, Belgium
2.Ghent Univ Hosp, Dept Surg, B-9000 Ghent, Belgium
3.Ghent Univ Hosp, Dept Pathol, B-9000 Ghent, Belgium
4.Univ Ghent, VIB, Dept Mol Biomed Res, B-9052 Ghent, Belgium
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Liu, Xue-en,Dewaele, Sylviane,Vanhooren, Valerie,et al. Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer[J]. LIVER INTERNATIONAL,2010,30(8):1221-1228.
APA Liu, Xue-en.,Dewaele, Sylviane.,Vanhooren, Valerie.,Fan, Ye-Dong.,Wang, Ling.,...&Chen, Cuiying Chitty.(2010).Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer.LIVER INTERNATIONAL,30(8),1221-1228.
MLA Liu, Xue-en,et al."Alteration of N-glycome in diethylnitrosamine-induced hepatocellular carcinoma mice: a non-invasive monitoring tool for liver cancer".LIVER INTERNATIONAL 30.8(2010):1221-1228.
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