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学科主题临床医学
Spred2 Modulates the Erythroid Differentiation Induced by Imatinib in Chronic Myeloid Leukemia Cells
Yang, Yuefeng1; Liu, Xiaoyun2; Xiao, Fengjun1; Xue, Shuya1; Xu, Qinqin5; Yin, Yue3; Sun, Huiyan1; Xu, Jie1; Wang, Hengxiang4; Zhang, Qunwei1; Wang, Hua1; Wang, Lisheng1
刊名PLOS ONE
2015-02-17
DOI10.1371/journal.pone.0117573
10期:2
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者Chinese National Basic Research and Development (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; Chinese National Basic Research and Development (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program)
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]CHRONIC MYELOGENOUS LEUKEMIA ; BCR-ABL ; MEGAKARYOCYTIC DIFFERENTIATION ; TRANSCRIPTION-FACTOR ; SIGNAL-TRANSDUCTION ; TYROSINE KINASE ; DOWN-REGULATION ; K562 ; ACTIVATION ; RESISTANCE
英文摘要

Differentiation induction is currently considered as an alternative strategy for treating chronic myelogenous leukemia (CML). Our previous work has demonstrated that Sprouty-related EVH1 domainprotein2 (Spred2) was involved in imatinib mediated cytotoxicity in CML cells. However, its roles in growth and lineage differentiation of CML cells remain unknown. In this study, we found that CML CD34(+) cells expressed lower level of Spred2 compared with normal hematopoietic progenitor cells, and adenovirus mediated restoration of Spred2 promoted the erythroid differentiation of CML cells. Imatinib could induce Spred2 expression and enhance erythroid differentiation in K562 cells. However, the imatinib induced erythroid differentiation could be blocked by Spred2 silence using lentiviral vector PLKO.1-shSpred2. Spred2 interference activated phosphorylated-ERK (p-ERK) and inhibited erythroid differentiation, while ERK inhibitor, PD98059, could restore the erythroid differentiation, suggesting Spred2 regulated the erythroid differentiation partly through ERK signaling. Furthermore, Spred2 interference partly restored p-ERK level leading to inhibition of erythroid differentiation in imatinib treated K562 cells. In conclusion, Spred2 was involved in erythroid differentiation of CML cells and participated in imatinib induced erythroid differentiation partly through ERK signaling.

语种英语
所属项目编号2012CB518205 ; 81170460 ; 2012AA02A211 ; SS2014AA020515
资助者Chinese National Basic Research and Development (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; Chinese National Basic Research and Development (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program)
WOS记录号WOS:000350322700058
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67687
专题北京大学第一临床医学院_血液内科
作者单位1.Qinghai Prov Peoples Hosp, Dept Oncol, Xining, Peoples R China
2.Lanzhou Command, Ctr Dis Control & Prevent, Lanzhou, Peoples R China
3.Gen Hosp Air Force, Dept Hematol, Beijing, Peoples R China
4.Beijing Inst Radiat Med, Dept Expt Hematol, Beijing, Peoples R China
5.Peking Univ, Hosp 1, Dept Hematol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Yang, Yuefeng,Liu, Xiaoyun,Xiao, Fengjun,et al. Spred2 Modulates the Erythroid Differentiation Induced by Imatinib in Chronic Myeloid Leukemia Cells[J]. PLOS ONE,2015,10(2).
APA Yang, Yuefeng.,Liu, Xiaoyun.,Xiao, Fengjun.,Xue, Shuya.,Xu, Qinqin.,...&Wang, Lisheng.(2015).Spred2 Modulates the Erythroid Differentiation Induced by Imatinib in Chronic Myeloid Leukemia Cells.PLOS ONE,10(2).
MLA Yang, Yuefeng,et al."Spred2 Modulates the Erythroid Differentiation Induced by Imatinib in Chronic Myeloid Leukemia Cells".PLOS ONE 10.2(2015).
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