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学科主题: 临床医学
题名:
Spred2 Modulates the Erythroid Differentiation Induced by Imatinib in Chronic Myeloid Leukemia Cells
作者: Yang, Yuefeng1; Liu, Xiaoyun2; Xiao, Fengjun1; Xue, Shuya1; Xu, Qinqin5; Yin, Yue3; Sun, Huiyan1; Xu, Jie1; Wang, Hengxiang4; Zhang, Qunwei1; Wang, Hua1; Wang, Lisheng1
刊名: PLOS ONE
发表日期: 2015-02-17
DOI: 10.1371/journal.pone.0117573
卷: 10, 期:2
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: CHRONIC MYELOGENOUS LEUKEMIA ; BCR-ABL ; MEGAKARYOCYTIC DIFFERENTIATION ; TRANSCRIPTION-FACTOR ; SIGNAL-TRANSDUCTION ; TYROSINE KINASE ; DOWN-REGULATION ; K562 ; ACTIVATION ; RESISTANCE
英文摘要:

Differentiation induction is currently considered as an alternative strategy for treating chronic myelogenous leukemia (CML). Our previous work has demonstrated that Sprouty-related EVH1 domainprotein2 (Spred2) was involved in imatinib mediated cytotoxicity in CML cells. However, its roles in growth and lineage differentiation of CML cells remain unknown. In this study, we found that CML CD34(+) cells expressed lower level of Spred2 compared with normal hematopoietic progenitor cells, and adenovirus mediated restoration of Spred2 promoted the erythroid differentiation of CML cells. Imatinib could induce Spred2 expression and enhance erythroid differentiation in K562 cells. However, the imatinib induced erythroid differentiation could be blocked by Spred2 silence using lentiviral vector PLKO.1-shSpred2. Spred2 interference activated phosphorylated-ERK (p-ERK) and inhibited erythroid differentiation, while ERK inhibitor, PD98059, could restore the erythroid differentiation, suggesting Spred2 regulated the erythroid differentiation partly through ERK signaling. Furthermore, Spred2 interference partly restored p-ERK level leading to inhibition of erythroid differentiation in imatinib treated K562 cells. In conclusion, Spred2 was involved in erythroid differentiation of CML cells and participated in imatinib induced erythroid differentiation partly through ERK signaling.

语种: 英语
所属项目编号: 2012CB518205 ; 81170460 ; 2012AA02A211 ; SS2014AA020515
项目资助者: Chinese National Basic Research and Development (973 Program) ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program)
WOS记录号: WOS:000350322700058
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67687
Appears in Collections:北京大学第一临床医学院_血液内科_期刊论文

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作者单位: 1.Qinghai Prov Peoples Hosp, Dept Oncol, Xining, Peoples R China
2.Lanzhou Command, Ctr Dis Control & Prevent, Lanzhou, Peoples R China
3.Gen Hosp Air Force, Dept Hematol, Beijing, Peoples R China
4.Beijing Inst Radiat Med, Dept Expt Hematol, Beijing, Peoples R China
5.Peking Univ, Hosp 1, Dept Hematol, Beijing 100871, Peoples R China

Recommended Citation:
Yang, Yuefeng,Liu, Xiaoyun,Xiao, Fengjun,et al. Spred2 Modulates the Erythroid Differentiation Induced by Imatinib in Chronic Myeloid Leukemia Cells[J]. PLOS ONE,2015,10(2).
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