IR@PKUHSC  > 北京大学第三临床医学院  > 药剂科
学科主题临床医学
Sensitive quantification of rifaximin in human plasma by liquid chromatography-tandem mass spectrometry
Zhang, Xianhua; Duan, Jingli; Li, Ke; Zhou, Liya; Zhai, Suodi
关键词rifaximin metoprolol LC-MS/MS human plasma
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2007-05-01
DOI10.1016/jjchromb.2006.12.001
850期:1-2页:348-355
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]RIFAMYCIN ; LACTULOSE
英文摘要

A liquid chromatography-mass spectrometry method (LC-MS/MS) for the quantitative determination of rifaximin in human plasma was developed and validated. In the developed procedure, metoprolol was added to human plasma as an internal standard (IS) and acetonitrile was used to precipitate the plasma proteins before LC-MS/MS analysis. Chromatographic separation was obtained on a RESTEK Pinnacle C18 column (50 mm x 2.1 mm, 5 mu m) with a mobile phase consisted of ammonium acetate solution (15 mM, pH 4.32) as buffer A and methanol as mobile phase B. Quantification was performed in positive mode using multiple reaction monitoring (MRM) of the transitions m/z 786.1 -> 754.1 for rifaximin and m/z 268.3 -> 116.1 for the IS. The assay has been validated over the concentration range of 0.5-10 ng/ml (r= 0.9992) based on the analysis of 0.2 ml of plasma. The assay accuracy was between 98.2% and 109%. The within-day and between-day precision was better than 3.9% and 8.9% at three concentration levels. The freeze-thaw stability was also investigated and it was found that both rifaximin and the IS were quite stable. This method provides a rapid, sensitive, specific and robust tool for the quantitative determination of rifaximin in human plasma, which is especially useful for the pharmacokinetic study of rifaximin. (c) 2006 Elsevier B.V All rights reserved.

语种英语
WOS记录号WOS:000246703500044
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67692
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Ctr Therapeut Drugs Monitoring & Clin Toxicol, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Xianhua,Duan, Jingli,Li, Ke,et al. Sensitive quantification of rifaximin in human plasma by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,850(1-2):348-355.
APA Zhang, Xianhua,Duan, Jingli,Li, Ke,Zhou, Liya,&Zhai, Suodi.(2007).Sensitive quantification of rifaximin in human plasma by liquid chromatography-tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,850(1-2),348-355.
MLA Zhang, Xianhua,et al."Sensitive quantification of rifaximin in human plasma by liquid chromatography-tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 850.1-2(2007):348-355.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Xianhua]的文章
[Duan, Jingli]的文章
[Li, Ke]的文章
百度学术
百度学术中相似的文章
[Zhang, Xianhua]的文章
[Duan, Jingli]的文章
[Li, Ke]的文章
必应学术
必应学术中相似的文章
[Zhang, Xianhua]的文章
[Duan, Jingli]的文章
[Li, Ke]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。