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学科主题: 临床医学
题名:
Sensitive quantification of rifaximin in human plasma by liquid chromatography-tandem mass spectrometry
作者: Zhang, Xianhua; Duan, Jingli; Li, Ke; Zhou, Liya; Zhai, Suodi
关键词: rifaximin ; metoprolol ; LC-MS/MS ; human plasma
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2007-05-01
DOI: 10.1016/jjchromb.2006.12.001
卷: 850, 期:1-2, 页:348-355
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: RIFAMYCIN ; LACTULOSE
英文摘要:

A liquid chromatography-mass spectrometry method (LC-MS/MS) for the quantitative determination of rifaximin in human plasma was developed and validated. In the developed procedure, metoprolol was added to human plasma as an internal standard (IS) and acetonitrile was used to precipitate the plasma proteins before LC-MS/MS analysis. Chromatographic separation was obtained on a RESTEK Pinnacle C18 column (50 mm x 2.1 mm, 5 mu m) with a mobile phase consisted of ammonium acetate solution (15 mM, pH 4.32) as buffer A and methanol as mobile phase B. Quantification was performed in positive mode using multiple reaction monitoring (MRM) of the transitions m/z 786.1 -> 754.1 for rifaximin and m/z 268.3 -> 116.1 for the IS. The assay has been validated over the concentration range of 0.5-10 ng/ml (r= 0.9992) based on the analysis of 0.2 ml of plasma. The assay accuracy was between 98.2% and 109%. The within-day and between-day precision was better than 3.9% and 8.9% at three concentration levels. The freeze-thaw stability was also investigated and it was found that both rifaximin and the IS were quite stable. This method provides a rapid, sensitive, specific and robust tool for the quantitative determination of rifaximin in human plasma, which is especially useful for the pharmacokinetic study of rifaximin. (c) 2006 Elsevier B.V All rights reserved.

语种: 英语
WOS记录号: WOS:000246703500044
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67692
Appears in Collections:北京大学第三临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 3, Ctr Therapeut Drugs Monitoring & Clin Toxicol, Beijing 100083, Peoples R China
3.Peking Univ, Hosp 3, Dept Gastroenterol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Xianhua,Duan, Jingli,Li, Ke,et al. Sensitive quantification of rifaximin in human plasma by liquid chromatography-tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2007,850(1-2):348-355.
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