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学科主题: 临床医学
题名:
Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer
作者: Chen, Zi-Yi1; Zhang, Jun-Ling1; Yao, Hong-Xin2; Wang, Peng-Yuan1; Zhu, Jing1; Wang, Wei1; Wang, Xin1; Wan, Yuan-Lian1; Chen, Shan-Wen1; Chen, Guo-Wei1; Liu, Yu-Cun1
刊名: SCIENTIFIC REPORTS
发表日期: 2014-12-17
DOI: 10.1038/srep07035
卷: 4
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: DNA METHYLATION ; POOR SURVIVAL ; EXPRESSION ; HYPERMETHYLATION ; PROTEIN ; CELLS ; PROGRESSION ; CARCINOMA ; PHENOTYPE ; RICH
英文摘要:

Secreted protein acidic and rich in cysteine (SPARC) gene has been shown to be epigenetically silenced in several cancers. We investigated the loss of expression and promoter methylation of this tumor suppressor gene in gastric cancers and correlated the data with clinicopathological features. We observed the loss of SPARC mRNA and SPARC protein expression in 7 of 10 (70%) gastric cancer cell lines. Upon treatment of expression-negative cell lines with a demethylating agent, expression of mRNA and protein was restored in all cells. Methylation rate of SPARC gene was 80% in ten gastric cancer cell lines and 74% (163 of 220) in primary tumors, while it was 5% in normal gastric mucosa (n = 40). In intestinal gastric cancer, SPARC methylation correlated with a negative prognosis (P < 0.001; relative risk 2.754, 95% confidence interval 1.780-4.261). Immunostaining revealed that SPARC protein was overexpressed in stromal fibroblasts adjacent to neoplastic epithelium but rarely expressed in the primary gastric cancer cells. These results implicate SPARC promoter methylation as an important factor in the tumorigenesis of gastric carcinomas and provide new insights into the potential use of SPARC as a novel biomarker and the potential clinical importance in human gastric cancers.

语种: 英语
所属项目编号: 30901417/H1617
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000346403300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67695
Appears in Collections:北京大学第一临床医学院_普通外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat Surg, Beijing 100034, Peoples R China

Recommended Citation:
Chen, Zi-Yi,Zhang, Jun-Ling,Yao, Hong-Xin,et al. Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer[J]. SCIENTIFIC REPORTS,2014,4.
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