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学科主题临床医学
Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer
Chen, Zi-Yi1; Zhang, Jun-Ling1; Yao, Hong-Xin2; Wang, Peng-Yuan1; Zhu, Jing1; Wang, Wei1; Wang, Xin1; Wan, Yuan-Lian1; Chen, Shan-Wen1; Chen, Guo-Wei1; Liu, Yu-Cun1
刊名SCIENTIFIC REPORTS
2014-12-17
DOI10.1038/srep07035
4
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]DNA METHYLATION ; POOR SURVIVAL ; EXPRESSION ; HYPERMETHYLATION ; PROTEIN ; CELLS ; PROGRESSION ; CARCINOMA ; PHENOTYPE ; RICH
英文摘要

Secreted protein acidic and rich in cysteine (SPARC) gene has been shown to be epigenetically silenced in several cancers. We investigated the loss of expression and promoter methylation of this tumor suppressor gene in gastric cancers and correlated the data with clinicopathological features. We observed the loss of SPARC mRNA and SPARC protein expression in 7 of 10 (70%) gastric cancer cell lines. Upon treatment of expression-negative cell lines with a demethylating agent, expression of mRNA and protein was restored in all cells. Methylation rate of SPARC gene was 80% in ten gastric cancer cell lines and 74% (163 of 220) in primary tumors, while it was 5% in normal gastric mucosa (n = 40). In intestinal gastric cancer, SPARC methylation correlated with a negative prognosis (P < 0.001; relative risk 2.754, 95% confidence interval 1.780-4.261). Immunostaining revealed that SPARC protein was overexpressed in stromal fibroblasts adjacent to neoplastic epithelium but rarely expressed in the primary gastric cancer cells. These results implicate SPARC promoter methylation as an important factor in the tumorigenesis of gastric carcinomas and provide new insights into the potential use of SPARC as a novel biomarker and the potential clinical importance in human gastric cancers.

语种英语
WOS记录号WOS:000346403300001
项目编号30901417/H1617
资助机构National Natural Science Foundation of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67695
专题北京大学第一临床医学院_普通外科
作者单位1.Peking Univ, Hosp 1, Dept Gen Surg, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat Surg, Beijing 100034, Peoples R China
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GB/T 7714
Chen, Zi-Yi,Zhang, Jun-Ling,Yao, Hong-Xin,et al. Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer[J]. SCIENTIFIC REPORTS,2014,4.
APA Chen, Zi-Yi.,Zhang, Jun-Ling.,Yao, Hong-Xin.,Wang, Peng-Yuan.,Zhu, Jing.,...&Liu, Yu-Cun.(2014).Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer.SCIENTIFIC REPORTS,4.
MLA Chen, Zi-Yi,et al."Aberrant methylation of the SPARC gene promoter and its clinical implication in gastric cancer".SCIENTIFIC REPORTS 4(2014).
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