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学科主题临床医学
Gene expression profile in streptozotocin-induced diabetic mice kidneys undergoing glomerulosclerosis
Wada, J; Zhang, H; Tsuchiyama, Y; Hiragushi, K; Hida, K; Shikata, K; Kanwar, YS; Makino, H
关键词CD-1 (ICR) mouse diabetic nephropathy hyperglycemia tubulointerstitial fibrosis end-stage renal failure
刊名KIDNEY INTERNATIONAL
2001-04-01
59期:4页:1363-1373
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Urology & Nephrology
研究领域[WOS]Urology & Nephrology
关键词[WOS]ACTIVATED PROTEIN-KINASE ; DNA HELICASE-II ; GLUCOSE-METABOLISM ; EXTRACELLULAR-MATRIX ; RENAL HYPERTROPHY ; EPITHELIAL-CELLS ; BINDING-PROTEIN ; MESSENGER-RNA ; MOUSE KIDNEY ; MELLITUS
英文摘要

Background. To elucidate the molecular mechanism of diabetic nephropathy, a high-density DNA filter array was employed to survey the gene expression profile of streptozotocin-induced diabetic CD-1 (ICR) mouse kidneys.

Methods. Ten-week-old CD-1 male mice were divided into four groups: (1) control, (2) unilaterally nephrectomized (UX) mice, (3) streptozotocin (STZ)-induced diabetic (STZ) mice, and (4) STZ mice with unilateral renal ablation (STZ-UX). Pathological changes were examined at 24 weeks after the induction. The gene expression profile was compared between the control and STZ mice by a Gene Discovery Array (GDA).

Results. The glomeruli in UX mouse kidney showed prominent glomerular hypertrophy, while the accumulation of mesangial matrix was minimal. Both STZ and STZ + UX mice had significant glomerular hypertrophy and glomerulosclerosis, and the lesions were not enhanced by renal ablation. By comparison between control and STZ mice, 16 clones that increased in expression with the induction of diabetes and 65 clones that decreased in diabetic kidneys were identified. The 37 known genes were related to glucose and lipid metabolism, ion transport, transcription factors, signaling molecules, and extracellular matrix-related molecules. The genes known to be involved in cell differentiation and organogenesis in various tissues (that is, Unc-18 homolog, POU domain transcription factor 2, lunatic fringe gene homolog, fibrous sheath component 1, Sox-17, fibulin 2, and MRJ) were found to be differentially expressed in the early phase of diabetic kidneys.

Conclusions. Hyperglycemia is a major determinant of glomerulosclerosis in STZ-induced diabetic CD-1 mice, and the altered gene expression in the early phase of diabetic kidney may be critical for the development of diabetic nephropathy.

语种英语
WOS记录号WOS:000167737200017
引用统计
被引频次:57[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67697
专题北京大学第一临床医学院_肾脏内科
作者单位1.Okayama Univ, Sch Med, Dept Med 3, Okayama 7008558, Japan
2.Beijing Med Univ, Teaching Hosp 1, Dept Nephrol, Beijing 100083, Peoples R China
3.Northwestern Univ, Sch Med, Dept Pathol, Chicago, IL 60611 USA
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GB/T 7714
Wada, J,Zhang, H,Tsuchiyama, Y,et al. Gene expression profile in streptozotocin-induced diabetic mice kidneys undergoing glomerulosclerosis[J]. KIDNEY INTERNATIONAL,2001,59(4):1363-1373.
APA Wada, J.,Zhang, H.,Tsuchiyama, Y.,Hiragushi, K.,Hida, K.,...&Makino, H.(2001).Gene expression profile in streptozotocin-induced diabetic mice kidneys undergoing glomerulosclerosis.KIDNEY INTERNATIONAL,59(4),1363-1373.
MLA Wada, J,et al."Gene expression profile in streptozotocin-induced diabetic mice kidneys undergoing glomerulosclerosis".KIDNEY INTERNATIONAL 59.4(2001):1363-1373.
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