IR@PKUHSC  > 北京大学第二临床医学院
学科主题临床医学
Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia
Qu, Jiuxin1; Gu, Li1; Wu, Jiang2; Dong, Jianping3; Pu, Zenghui4; Gao, Yan5; Hu, Ming6; Zhang, Yongxiang7; Gao, Feng8; Cao, Bin1; Wang, Chen9; BNACAP
关键词Community-acquired pneumonia Mycoplasma pneumoniae Diagnosis Adult
刊名BMC INFECTIOUS DISEASES
2013-04-11
DOI10.1186/1471-2334-13-172
13
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Infectious Diseases
资助者Beijing Science and Technology Project ; New Century Excellent Talents in University ; Beijing Municipal Health Bureau ; Beijing Science and Technology Project ; New Century Excellent Talents in University ; Beijing Municipal Health Bureau
研究领域[WOS]Infectious Diseases
关键词[WOS]RESPIRATORY-TRACT INFECTIONS ; SEROLOGY
英文摘要

Background: Diagnosis of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae in adults and adolescents is hampered by a lack of rapid and standardized tests for detection.

Methods: CAP patients from 12 teaching hospitals were prospectively and consecutively recruited. Basic and clinical information, throat swabs and paired sera were collected. Mycoplasma pneumoniae was detected by IgG and IgM antibody tests, fluorescence quantitative polymerase chain reaction (FQ-PCR) and culture. A comparative study of the diagnostic values of three methods, including sensitivity, specificity, positive and negative predictive values and positive likelihood ratio (PLR) was conducted. A fourfold or greater increase of IgG antibody titers of paired sera was set as the diagnostic "gold standard".

Results: One hundred and twenty-five CAP patients (52.8% males, median age 47 years, range 14-85) were enrolled. Twenty-seven (21.6%) patients were diagnosed with acute Mycoplasma pneumoniae infections by the "gold standard". Specificity values of all three methods were around 90%. An increasing trend of sensitivity, positive predictive value and PLR was found, with the lowest in IgM testing (7.4%, 28.6% and 1.45), intermediate in FQ-PCR (40.7%, 50% and 3.63), and highest in culture (55.6%, 75% and 10.9).

Conclusions: In the defined group of patients, there was a good agreement between positive rate of MP cultivation of throat swabs and acute M. pneumoniae infection (PLR of 10.9). Since the sensitivity is low in all of the evaluated methods, the logical approach would be to incorporate PCR, culture and serological tests for optimum diagnosis of acute Mycoplasma pneumoniae infections in adults and adolescents.

语种英语
所属项目编号D101100049810002 ; NCET-10-0006 ; 2011-1004-02
资助者Beijing Science and Technology Project ; New Century Excellent Talents in University ; Beijing Municipal Health Bureau ; Beijing Science and Technology Project ; New Century Excellent Talents in University ; Beijing Municipal Health Bureau
WOS记录号WOS:000318559900001
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67706
专题北京大学第二临床医学院
作者单位1.Capital Med Univ, Beijing Chao Yang Hosp, Beijing Inst Resp Med, Dept Infect Dis & Clin Microbiol, Beijing 100020, Peoples R China
2.Beijing Ctr Dis Control & Prevent, Beijing 100020, Peoples R China
3.Beijing Hai Dian Hosp, Beijing 100020, Peoples R China
4.Yan Tai Yu Huang Ding Hosp, Beijing 100020, Peoples R China
5.Peking Univ, Peoples Hosp, Beijing 100020, Peoples R China
6.Capital Med Univ, Lu He teaching Hosp, Beijing 100020, Peoples R China
7.Beijing Da Xing Hosp, Beijing 100020, Peoples R China
8.Chinese Acad Chinese Med Sci, WangJing Hosp, Beijing 100020, Peoples R China
9.Beijing Hosp, Beijing Inst Resp Med, Minist Hlth, Beijing 100020, Peoples R China
推荐引用方式
GB/T 7714
Qu, Jiuxin,Gu, Li,Wu, Jiang,et al. Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia[J]. BMC INFECTIOUS DISEASES,2013,13.
APA Qu, Jiuxin.,Gu, Li.,Wu, Jiang.,Dong, Jianping.,Pu, Zenghui.,...&BNACAP.(2013).Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia.BMC INFECTIOUS DISEASES,13.
MLA Qu, Jiuxin,et al."Accuracy of IgM antibody testing, FQ-PCR and culture in laboratory diagnosis of acute infection by Mycoplasma pneumoniae in adults and adolescents with community-acquired pneumonia".BMC INFECTIOUS DISEASES 13(2013).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Qu, Jiuxin]的文章
[Gu, Li]的文章
[Wu, Jiang]的文章
百度学术
百度学术中相似的文章
[Qu, Jiuxin]的文章
[Gu, Li]的文章
[Wu, Jiang]的文章
必应学术
必应学术中相似的文章
[Qu, Jiuxin]的文章
[Gu, Li]的文章
[Wu, Jiang]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。