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学科主题: 临床医学
题名:
Monitoring MRD with flow cytometry: an effective method to predict relapse for ALL patients after allogeneic hematopoietic stem cell transplantation
作者: Zhao, Xiao-Su; Liu, Yan-Rong; Zhu, Hong-Hu; Xu, Lan-Ping; Liu, Dai-Hong; Liu, Kai-Yan; Huang, Xiao-Jun
关键词: Minimal residual disease ; Acute lymphoblastic leukemia ; Relapse ; Hematopoietic stem cell transplantation ; Flow cytometry
刊名: ANNALS OF HEMATOLOGY
发表日期: 2012-02-01
DOI: 10.1007/s00277-011-1285-1
卷: 91, 期:2, 页:183-192
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology
研究领域[WOS]: Hematology
关键词[WOS]: MINIMAL RESIDUAL DISEASE ; ACUTE LYMPHOBLASTIC-LEUKEMIA ; BONE-MARROW-TRANSPLANTATION ; POLYMERASE-CHAIN-REACTION ; CLINICAL-SIGNIFICANCE ; ADULT PATIENTS ; CHILDREN ; QUANTIFICATION ; CLEARANCE ; PCR
英文摘要:

This study evaluated the prognostic value of minimal residual disease (MRD) monitoring by four-color flow cytometry (FCM) in patients with acute lymphoblastic leukemia (ALL) undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). MRD was examined with four-color FCM at different time points in 139 patients (including pediatric and adult patients) with ALL after allo-HSCT. Real-time quantitative polymerase chain reaction (RQ-PCR) was applied to evaluate the MRD of Philadelphia chromosome-positive ALL (Ph+ ALL) patients. Patients who were FCM-positive (FCM+) after transplantation had a lower event-free survival (EFS) of 0.54 and a higher cumulative incidence of relapse (CIR) of 0.54 compared to an EFS of 0.80 and a CIR of 0.08 in FCM-negative (FCM-) patients (EFS, p < 0.001; CIR, p < 0.001). Similar results were obtained in high-risk patients and Ph+ ALL patients. Moreover, a FCM+ status after the second month post-HSCT (defined as MRD positive) proved to be a predictor of leukemia relapse. Multivariate analysis for EFS, OS and CIR showed that MRD status after transplantation was an independent prognostic factor (p < 0.001, p = 0.013, and p < 0.001, respectively). A good correlation was found between the MRD results of FCM and RQ-PCR (n = 126 pairs, Spearman r = 0.8139, p < 0.001). MRD monitoring by four-color FCM post-transplantation is an important tool for relapse prediction in ALL patients. Prompt and appropriate pre-emptive anti-leukemia treatment could be considered based on the status of MRD after HSCT.

语种: 英语
所属项目编号: 30725038 ; IRT0702
项目资助者: National Outstanding Young Scientist&prime ; s Foundation of China ; Program for Innovative Research Team in University ; Clinical Faculty
WOS记录号: WOS:000299055000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67716
Appears in Collections:北京大学第二临床医学院_血液科_期刊论文

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作者单位: Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Zhao, Xiao-Su,Liu, Yan-Rong,Zhu, Hong-Hu,et al. Monitoring MRD with flow cytometry: an effective method to predict relapse for ALL patients after allogeneic hematopoietic stem cell transplantation[J]. ANNALS OF HEMATOLOGY,2012,91(2):183-192.
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