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Monitoring MRD with flow cytometry: an effective method to predict relapse for ALL patients after allogeneic hematopoietic stem cell transplantation
Zhao, Xiao-Su; Liu, Yan-Rong; Zhu, Hong-Hu; Xu, Lan-Ping; Liu, Dai-Hong; Liu, Kai-Yan; Huang, Xiao-Jun
关键词Minimal residual disease Acute lymphoblastic leukemia Relapse Hematopoietic stem cell transplantation Flow cytometry
刊名ANNALS OF HEMATOLOGY
2012-02-01
DOI10.1007/s00277-011-1285-1
91期:2页:183-192
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Hematology
研究领域[WOS]Hematology
关键词[WOS]MINIMAL RESIDUAL DISEASE ; ACUTE LYMPHOBLASTIC-LEUKEMIA ; BONE-MARROW-TRANSPLANTATION ; POLYMERASE-CHAIN-REACTION ; CLINICAL-SIGNIFICANCE ; ADULT PATIENTS ; CHILDREN ; QUANTIFICATION ; CLEARANCE ; PCR
英文摘要

This study evaluated the prognostic value of minimal residual disease (MRD) monitoring by four-color flow cytometry (FCM) in patients with acute lymphoblastic leukemia (ALL) undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). MRD was examined with four-color FCM at different time points in 139 patients (including pediatric and adult patients) with ALL after allo-HSCT. Real-time quantitative polymerase chain reaction (RQ-PCR) was applied to evaluate the MRD of Philadelphia chromosome-positive ALL (Ph+ ALL) patients. Patients who were FCM-positive (FCM+) after transplantation had a lower event-free survival (EFS) of 0.54 and a higher cumulative incidence of relapse (CIR) of 0.54 compared to an EFS of 0.80 and a CIR of 0.08 in FCM-negative (FCM-) patients (EFS, p < 0.001; CIR, p < 0.001). Similar results were obtained in high-risk patients and Ph+ ALL patients. Moreover, a FCM+ status after the second month post-HSCT (defined as MRD positive) proved to be a predictor of leukemia relapse. Multivariate analysis for EFS, OS and CIR showed that MRD status after transplantation was an independent prognostic factor (p < 0.001, p = 0.013, and p < 0.001, respectively). A good correlation was found between the MRD results of FCM and RQ-PCR (n = 126 pairs, Spearman r = 0.8139, p < 0.001). MRD monitoring by four-color FCM post-transplantation is an important tool for relapse prediction in ALL patients. Prompt and appropriate pre-emptive anti-leukemia treatment could be considered based on the status of MRD after HSCT.

语种英语
WOS记录号WOS:000299055000005
项目编号30725038 ; IRT0702
资助机构National Outstanding Young Scientist&prime ; s Foundation of China ; Program for Innovative Research Team in University ; Clinical Faculty
引用统计
被引频次:49[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67716
专题北京大学第二临床医学院_血液科
作者单位Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100044, Peoples R China
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GB/T 7714
Zhao, Xiao-Su,Liu, Yan-Rong,Zhu, Hong-Hu,et al. Monitoring MRD with flow cytometry: an effective method to predict relapse for ALL patients after allogeneic hematopoietic stem cell transplantation[J]. ANNALS OF HEMATOLOGY,2012,91(2):183-192.
APA Zhao, Xiao-Su.,Liu, Yan-Rong.,Zhu, Hong-Hu.,Xu, Lan-Ping.,Liu, Dai-Hong.,...&Huang, Xiao-Jun.(2012).Monitoring MRD with flow cytometry: an effective method to predict relapse for ALL patients after allogeneic hematopoietic stem cell transplantation.ANNALS OF HEMATOLOGY,91(2),183-192.
MLA Zhao, Xiao-Su,et al."Monitoring MRD with flow cytometry: an effective method to predict relapse for ALL patients after allogeneic hematopoietic stem cell transplantation".ANNALS OF HEMATOLOGY 91.2(2012):183-192.
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