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IR@PKUHSC  > 北京大学临床肿瘤学院  > 分子肿瘤学研究室  > 期刊论文
学科主题: 临床医学
题名:
Matrix metalloproteinase 11 depletion inhibits cell proliferation in gastric cancer cells
作者: Deng, H; Guo, RF; Li, WM; Zhao, M; Lu, YY
关键词: MMP11 ; gastric cancer ; RNAi ; proliferation
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2005-01-14
DOI: 10.1016/j.bbrc.2004.11.027
卷: 326, 期:2, 页:274-281
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: HUMAN STROMELYSIN-3 ; BREAST-CANCER ; TUMOR-CELL ; ATM GENE ; EXPRESSION ; METALLOPROTEINASE ; CARCINOMAS ; LINES ; PROGRESSION ; ACTIVATION
英文摘要:

Our previous study has shown that matrix metalloproteinase 11 (MMP11) is highly expressed in tumor cell lines and primary tumor of gastric cancer (GC). In order to reveal the correlation between expression of MMP11 and biological features of GC cell, we have constructed the recombinant plasmids producing hairpin small interfering RNA (siRNA) to target MMP11 mRNA using a vector-based RNA interference technology. Stable transfection of recombinants into GC cell line BGC823 specifically depleted the mRNA and protein of MMP11 as demonstrated by RT-PCR and Western blotting analysis. The siRNA-treated cells exhibited significantly decreased growth ability compared with mock transfectants and parental BGC823 cells. Furthermore, colony formation of MMP11 deficient cells was dramatically inhibited in soft agar and tumorigenicity was reduced in nude mice, respectively. These results provide new insights into the function of MMP11 and suggest that MMP11 may play an important role in the control of cell proliferation and tumor development in GC. (C) 2004 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000225997300003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67717
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

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作者单位: Peking Univ, Mol Oncol Lab, Inst Canc Res, Sch Oncol, Beijing 100034, Peoples R China

Recommended Citation:
Deng, H,Guo, RF,Li, WM,et al. Matrix metalloproteinase 11 depletion inhibits cell proliferation in gastric cancer cells[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2005,326(2):274-281.
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