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学科主题: 药学
题名:
Characteristics and biodistribution of soybean sterylglucoside and polyethylene glycol-modified cationic liposomes and their complexes with antisense oligodeoxynucleotide
作者: Shi, J; Yan, WW; Qi, XR; Maitani, Y; Nagai, T
关键词: antisense oligodeoxynucleotide ; cationic liposomes ; hepatocytes ; pegylated ; soybean sterylglucoside
刊名: DRUG DELIVERY
发表日期: 2005-11-01
DOI: 10.1080/10717540590968215
卷: 12, 期:6, 页:349-356
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: HEPATITIS-B-VIRUS ; GENE-TRANSFER ; IN-VIVO ; INHIBITION ; CELLS ; OLIGONUCLEOTIDES ; EXPRESSION ; SURFACE ; PHOSPHATIDYLETHANOLAMINE ; STABILITY
英文摘要:

A novel cationic liposome modified with soybean sterylglucoside (SG) and polyethylene glycol-distearoylphosphatidylethanolamine (PEG-DSPE) as a carrier of antisense oligodeoxynucleotide (ODN) for hepatitis B virus (HBV) therapy was constructed. Characteristics of the cationic liposomes modified with SG and PEG (SG/PEG-CL) and their complexes with 15-mer phosphorothioate ODN (SG/PEG-CL-ODN complex) were investigated by incorporation efficiency, morphology, electrophoresis, zeta potentials, and size analysis. Antisense activity of the liposomes and ODN complexes was determined as hepatitis B surface antigen ( HBsAg) and hepatitis B e antigen ( HBeAg) in HepG(2) 2.2.15 cells by ELISA. Their tissue and intrahepatic distribution were evaluated following intravenous injection in mice. The complexes gained high incorporation efficiency and intact vesicular structure with mean size at similar to 200 nm. The SG/PEG-CL-ODN complexes enhanced the inhibition of both HBsAg and HBeAg expression in the cultured HepG2 2.2.15 cells relative to free ODN. The uptake of SG/PEG-CL and nonmodified cationic liposomes ( CL) was primarily by liver, spleen, and lung. Furthermore, the concentration of SG/PEG-CL was significant higher than that of CL in hepatoctyes at 0.5 hr postinjection. The biodistribution of SG/DSPE-CL-ODN complex compare with free ODN showed that liposomes enhanced the accumulation of ODN in the liver and spleen, while decreasing its blood concentration. SG/PEG-CL-mediated ODN transfer to the liver is an effective gene delivery method for cell-specific targeting, which has a potential for gene therapy of HBV infections. SG and PEG-modified cationic liposomes have proven to be an alternative carrier for hepatocyte-selective drug targeting.

语种: 英语
WOS记录号: WOS:000232882700003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67725
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Hoshi Univ, Inst Med Chem, Shinagawa Ku, Tokyo 142, Japan
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China

Recommended Citation:
Shi, J,Yan, WW,Qi, XR,et al. Characteristics and biodistribution of soybean sterylglucoside and polyethylene glycol-modified cationic liposomes and their complexes with antisense oligodeoxynucleotide[J]. DRUG DELIVERY,2005,12(6):349-356.
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