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学科主题: 药学
题名:
Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense
作者: Han, Xiao-Qiang1,2; Chan, Ben Chung Lap2; Yu, Hua2,3; Yang, Yin-Hua1; Hu, Shui-Qing2; Ko, Chun-Hay2; Dong, Cai-Xia3; Wong, Chun-Kwok2,6; Shaw, Pang-Chui2,4,5; Fung, Kwok-Pui2; Leung, Ping-Chung2; Hsiao, Wen-Luan3; Tu, Peng-Fei1; Han, Quan-Bin2,3
关键词: Ganoderma sinense ; Polysaccharide ; Immunological activities ; Human peripheral blood mononuclear cells ; Macrophages
刊名: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
发表日期: 2012-11-01
DOI: 10.1016/j.ijbiomac.2012.06.029
卷: 51, 期:4, 页:597-603
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: EDIBLE MUSHROOM ; FUNGAL ; FOODS
英文摘要:

A protein-bound polysaccharide (GSP-4) with a molecular weight of 8.3 x 10(5) Da, was isolated from the water extract of the fruiting bodies of Ganoderma sinense. Chemical study revealed that this fraction was composed of mannose, glucose and galactose in the molar ratio of 4.7:27.1:1.0, with the sugar residues of t-, 1,3-, 1,4-, 1,6-, 1,3,4- and 1,3.6-linked Glcp, t-linked Galp, and 1,6-linked Manp. The immnomodulatory effects of GSP-4 were assessed using human peripheral blood mononuclear cells (PBMCs) and murine monocyte/macrophage cell line RAW 264.7. We found that GSP-4 could significantly stimulate the production of the immunomodulatory markers tumor necrosis factor alpha (INF-alpha), interleukin (IL)-1 beta, IL-12, and granulocyte-macrophage colony-stimulating factor (GM-CSF) in PBMCs. This observation was further substantiated in RAW 264.7 cells, as indicated by the increase of nitric oxide (NO), TNF-alpha and IL-6 production. GSP-4 also enhanced the expression of inducible NO synthase mRNA in dose-dependent manner. Our current finding gives the first piece of evidence to support that GSP-4 possesses some promising immunomodulating effects and it could be a potential candidate to be further used in related cancer immunotherapy. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: ITS/311/09 ; InP/108/10 ; FRG2/11-12/048 ; SEG CUHK08 ; CUHK09
项目资助者: Innovation and Technology Fund of the Government of the Hong Kong Special Administrative Region ; Hong Kong Baptist University ; One-Off Special Equipment Grant of the University Grants Council of Hong Kong SAR
WOS记录号: WOS:000309310500036
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67731
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Chinese Univ Hong Kong, Inst Chinese Med, State Key Lab Phytochem & Plant Resources W China, Shatin, Hong Kong, Peoples R China
3.Hong Kong Baptist Univ, Sch Chinese Med, Hong Kong, Hong Kong, Peoples R China
4.Chinese Univ Hong Kong, Sch Life Sci, Shatin, Hong Kong, Peoples R China
5.Chinese Univ Hong Kong, Ctr Prot Sci & Crystallog, Shatin, Hong Kong, Peoples R China
6.Chinese Univ Hong Kong, Dept Chem Pathol, Prince Wales Hosp, Shatin, Hong Kong, Peoples R China

Recommended Citation:
Han, Xiao-Qiang,Chan, Ben Chung Lap,Yu, Hua,et al. Structural characterization and immuno-modulating activities of a polysaccharide from Ganoderma sinense[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2012,51(4):597-603.
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