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学科主题: 基础医学
题名:
Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M prostate cancer cells
作者: Pan, Xue-Yang; Guo, Hao; Han, Jing; Hao, Feng; An, Yu; Xu, Yan; Xiaokaiti, Yilixiati; Pan, Yan; Li, Xue-Jun1
关键词: Ginsenoside Rg3 ; Cell migration ; PC-3M cells ; Aquaporin 1 ; p38 MAPK ; Deletion analysis
刊名: EUROPEAN JOURNAL OF PHARMACOLOGY
发表日期: 2012-05-15
DOI: 10.1016/j.ejphar.2012.02.040
卷: 683, 期:1-3, 页:27-34
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: HYPERTONICITY-RESPONSIVE ELEMENT ; WATER CHANNEL ; IN-VITRO ; NATURAL-PRODUCTS ; AQP1 EXPRESSION ; METASTASIS ; PATHWAYS ; RG(3) ; SAPONINS ; PROMOTER
英文摘要:

Ginsenoside Rg3 (Rg3), one of the bioactive extracts found in ginseng root, was reported to have anti-cancer activity in various cancer models. The anti-proliferation effect of Rg3 on prostate cancer cells has been well reported. To test whether Rg3 has an anti-metastatic effect on prostate cancer, we treated a highly metastatic PC-3M prostate cancer cell line with Rg3. We found that Rg3 (10 mu M) led to remarkable inhibition of PC-3M cell migration. Simultaneously, exposure to Rg3 suppressed expression of the aquaporin 1 (AQP1) water channel protein, which has previously been reported to be involved in cell migration. Overexpression of AQP1 attenuated Rg3-induced inhibition of cell migration, and introduction of a shRNA targeting AQP1 abrogated the inhibitory effect of Rg3, although the basal level of cell migration was decreased by RNA interference. In mechanism study, estrogen receptor-and glucocorticoid receptor-dependent pathways are proved uninvolved in the AQP1 regulation by Rg3. However, Rg3 treatment triggered the activation of p38 MAPK; and SB202190, a specific inhibitor of p38 MAPK, antagonized the Rg3-induced regulation of AQP1 and cell migration, suggesting a crucial role for p38 in the regulation process. Deletion analysis of the promoter region of AQP1 was also conducted using dual-luciferase assay, which indicated that the -1000 bp to -200 bp promoter region was involved in the AQP1 regulation by Rg3. In all, we conclude that Rg3 effectively suppresses migration of PC-3M cells by down-regulating AQP1 expression through p38 MAPK pathway and some transcription factors acting on the AQP1 promoter. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81020108031 ; 30572202 ; 30973558 ; 30772571 ; 30901815 ; 30901803 ; 2009ZX09103-144 ; B07001
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China
WOS记录号: WOS:000303436200004
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67747
Appears in Collections:基础医学院_药理学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Pan, Xue-Yang,Guo, Hao,Han, Jing,et al. Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M prostate cancer cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2012,683(1-3):27-34.
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