IR@PKUHSC  > 北京大学基础医学院  > 药理学系
学科主题基础医学
Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M prostate cancer cells
Pan, Xue-Yang; Guo, Hao; Han, Jing; Hao, Feng; An, Yu; Xu, Yan; Xiaokaiti, Yilixiati; Pan, Yan; Li, Xue-Jun1
关键词Ginsenoside Rg3 Cell migration PC-3M cells Aquaporin 1 p38 MAPK Deletion analysis
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2012-05-15
DOI10.1016/j.ejphar.2012.02.040
683期:1-3页:27-34
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]HYPERTONICITY-RESPONSIVE ELEMENT ; WATER CHANNEL ; IN-VITRO ; NATURAL-PRODUCTS ; AQP1 EXPRESSION ; METASTASIS ; PATHWAYS ; RG(3) ; SAPONINS ; PROMOTER
英文摘要

Ginsenoside Rg3 (Rg3), one of the bioactive extracts found in ginseng root, was reported to have anti-cancer activity in various cancer models. The anti-proliferation effect of Rg3 on prostate cancer cells has been well reported. To test whether Rg3 has an anti-metastatic effect on prostate cancer, we treated a highly metastatic PC-3M prostate cancer cell line with Rg3. We found that Rg3 (10 mu M) led to remarkable inhibition of PC-3M cell migration. Simultaneously, exposure to Rg3 suppressed expression of the aquaporin 1 (AQP1) water channel protein, which has previously been reported to be involved in cell migration. Overexpression of AQP1 attenuated Rg3-induced inhibition of cell migration, and introduction of a shRNA targeting AQP1 abrogated the inhibitory effect of Rg3, although the basal level of cell migration was decreased by RNA interference. In mechanism study, estrogen receptor-and glucocorticoid receptor-dependent pathways are proved uninvolved in the AQP1 regulation by Rg3. However, Rg3 treatment triggered the activation of p38 MAPK; and SB202190, a specific inhibitor of p38 MAPK, antagonized the Rg3-induced regulation of AQP1 and cell migration, suggesting a crucial role for p38 in the regulation process. Deletion analysis of the promoter region of AQP1 was also conducted using dual-luciferase assay, which indicated that the -1000 bp to -200 bp promoter region was involved in the AQP1 regulation by Rg3. In all, we conclude that Rg3 effectively suppresses migration of PC-3M cells by down-regulating AQP1 expression through p38 MAPK pathway and some transcription factors acting on the AQP1 promoter. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000303436200004
项目编号81020108031 ; 30572202 ; 30973558 ; 30772571 ; 30901815 ; 30901803 ; 2009ZX09103-144 ; B07001
资助机构National Natural Science Foundation of China ; Ministry of Science and Technology in China ; Ministry of Education of China
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67747
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China
2.Peking Univ, Sch Basic Med Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Pan, Xue-Yang,Guo, Hao,Han, Jing,et al. Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M prostate cancer cells[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2012,683(1-3):27-34.
APA Pan, Xue-Yang.,Guo, Hao.,Han, Jing.,Hao, Feng.,An, Yu.,...&Li, Xue-Jun.(2012).Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M prostate cancer cells.EUROPEAN JOURNAL OF PHARMACOLOGY,683(1-3),27-34.
MLA Pan, Xue-Yang,et al."Ginsenoside Rg3 attenuates cell migration via inhibition of aquaporin 1 expression in PC-3M prostate cancer cells".EUROPEAN JOURNAL OF PHARMACOLOGY 683.1-3(2012):27-34.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Pan, Xue-Yang]的文章
[Guo, Hao]的文章
[Han, Jing]的文章
百度学术
百度学术中相似的文章
[Pan, Xue-Yang]的文章
[Guo, Hao]的文章
[Han, Jing]的文章
必应学术
必应学术中相似的文章
[Pan, Xue-Yang]的文章
[Guo, Hao]的文章
[Han, Jing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。