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Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis
Ling, Xiaomei; Liu, Yi; Fan, Hui; Zhong, Yingcheng; Li, Dan; Wang, Ying
关键词capillary zone electrophoresis PDCD5 heparin VV8 tat-NBD
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2007-02-01
DOI10.1007/s00216-006-0983-3
387期:3页:909-916
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]B KINASE COMPLEX ; CELLULAR UPTAKE ; GROWTH-FACTOR ; BINDING ; NEMO ; INTERNALIZATION ; PROTEOGLYCANS ; ACTIVATION ; COMPONENT ; SUBUNIT
英文摘要

Capillary zone electrophoresis (CZE) was used to investigate interactions between heparin and programmed cell death 5 (PDCD5), and between heparin and PDCD5-related peptides. Samples containing PDCD5, PDCD5-related peptides, and heparin at various ratios were incubated at room temperature and then separated by CZE with tris-acetate buffer at pH 7.2. Both qualitative and quantitative characterizations of the binding of PDCD5 and PDCD5-related peptides to heparin were determined. The changes in the signals of PDCD5 and PDCD5-related peptides were monitored by comparing the electropherograms of the mixtures containing PDCD5 and heparin and PDCD5-related peptides and heparin with that of PDCD5 or PDCD5-related peptides only. The binding constant of the interaction between PDCD5 and heparin was calculated as 4.17 x 10(4) M(-1)p by Scatchard analysis. Our investigations show that it is possible to characterize the interaction between PDCD5 and heparin quantitatively and the interaction between PDCD5-related peptides and heparin qualitatively using CZE.

语种英语
WOS记录号WOS:000243815500019
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67753
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100083, Peoples R China
2.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Ling, Xiaomei,Liu, Yi,Fan, Hui,et al. Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2007,387(3):909-916.
APA Ling, Xiaomei,Liu, Yi,Fan, Hui,Zhong, Yingcheng,Li, Dan,&Wang, Ying.(2007).Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,387(3),909-916.
MLA Ling, Xiaomei,et al."Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 387.3(2007):909-916.
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