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学科主题: 临床医学
题名:
Radiosensitivity of prostate cancer cells is enhanced by EGFR inhibitor C225
作者: Liu, Feng1; Wang, Jun-Jie1; You, Zhen-Yu1; Zhang, Ying-Dong1; Zhao, Yong2
关键词: EGFR inhibitor ; Prostate cancer cells ; Cell proliferation ; Apoptosis
刊名: UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
发表日期: 2010
DOI: 10.1016/j.urolonc.2008.07.001
卷: 28, 期:1, 页:59-66
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Urology & Nephrology
研究领域[WOS]: Oncology ; Urology & Nephrology
关键词[WOS]: GROWTH-FACTOR RECEPTOR ; MODULATED RADIATION-THERAPY ; ACTIVATED PROTEIN-KINASE ; SURVIVIN EXPRESSION ; MAPK PATHWAY ; LUNG-CANCER ; NECK-CANCER ; COMBINATION ; APOPTOSIS ; BINDING
英文摘要:

Purpose: To determine the direct effects of the epidermal growth factor receptor (EGFR) inhibitor C225 on the radiosensitivity of human prostate cancer cells.

Experimental design: Human prostate cancer DU145 cells were irradiated with (60)Co (1.953 Gy/min) at various doses in the presence or absence of C225. The cellular proliferation and cell-survival rate were evaluated by MTT and colony-forming assays after irradiation. The cell-cycle distribution, cell apoptosis, and MAPK expression were investigated using FCM. The expression of Cyclin D1, CDK2, CDK4, and Survivin were determined by RT-PCR.

Results: The RBE in the C225 group compared with that in the control group was 1.39. Cells treated with C225 and irradiated at 4 Gy predominantly exhibited GIG, phase arrest and significant decrease in the fraction of cells in the S phase in comparison with those in the control cells, respectively. An evidently higher apoptosis rate on irradiation at 4 Gy was observed in C225-treated cells compared with that in the control cells. Decreased cell proliferation and increased cell death were further supported by the down-regulation of cyclin D1, CDK2, CDK4, and survivin in C225-treated DU145 cells, as determined by RT-PCR. Furthermore, C225 significantly inhibited the phosphorylation of P38-MAPK in DU145 cells.

Conclusions: The EGER inhibitor C225 increased the radiosensitivity of DU145 cells through antiproliferative effect, inhibition of clonal growth, G(0)/G(1) phase arrest, apoptosis induction, and inhibition of EGFR-signaling pathways by the down-regulation of MAPK activation. (C) 2010 Published by Elsevier Inc.

语种: 英语
WOS记录号: WOS:000278948500009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67755
Appears in Collections:北京大学第三临床医学院_肿瘤放疗科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Ctr Canc, Beijing 100871, Peoples R China
2.Chinese Acad Sci, Inst Zool, State Key Lab Biomembrane & Membrane Biotechnol, Transplantat Biol Res Div, Beijing, Peoples R China

Recommended Citation:
Liu, Feng,Wang, Jun-Jie,You, Zhen-Yu,et al. Radiosensitivity of prostate cancer cells is enhanced by EGFR inhibitor C225[J]. UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS,2010,28(1):59-66.
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