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学科主题基础医学
The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes
Chen, XQ; Yu, ACH
关键词14-3-3 gamma actin astrocyte ischemia cell division mitosis apoptosis
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2002-08-23
296期:3页:657-663
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]ADRENAL CHROMAFFIN CELLS ; PROTEIN-KINASE ; MOLECULAR-CLONING ; 14-3-3-PROTEINS ; ISCHEMIA ; EXPRESSION ; SIGNAL ; RAF-1
英文摘要

The 14-3-3 protein family plays critical regulatory roles in signaling pathways in cell division and apoptosis. 14-3-3gamma is mainly expressed in brain. Using primary cultures of cerebral cortical astrocytes, we investigated the relationships between 14-3-3gamma proteins and actin in astrocytes in cell division and under ischemia. Our results showed that endogenous 14-3-3gamma proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). During certain stages of mitosis, 14-3-3gamma proteins first aggregated and then formed a ring-like structure that surrounded the daughter nuclei and enclosed the F-actin. In 4-week-old cultures of astrocytes, 14-3-3gamma proteins appeared as punctate aggregates in the cytoplasm. Under ischemia, 14-3-3gamma proteins formed filamentous structures and were closely associated with F-actin in surviving astrocytes. However, in apoptotic astrocytes, the intensity of immunostaining of 14-3-3gamma proteins in the cytoplasm decreased. The proteins aggregated around the nucleus and dissociated from the actin filaments. Reciprocal co-immunoprecipitations demonstrated that endogenous 14-3-3gamma proteins bound to detergent-soluble actin and the level of binding increased after 4 h of ischemia. As actin is a critical structural protein heavily involved in cell division and apoptotic death, our findings suggest that 14-3-3gamma proteins play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. (C) 2002 Elsevier Science (USA). All rights reserved.

语种英语
WOS记录号WOS:000177747800023
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被引频次:33[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67757
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China
4.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
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Chen, XQ,Yu, ACH. The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,296(3):657-663.
APA Chen, XQ,&Yu, ACH.(2002).The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,296(3),657-663.
MLA Chen, XQ,et al."The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 296.3(2002):657-663.
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