北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 神经生物学系  > 期刊论文
学科主题: 基础医学
题名:
The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes
作者: Chen, XQ; Yu, ACH
关键词: 14-3-3 gamma ; actin ; astrocyte ; ischemia ; cell division ; mitosis ; apoptosis
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2002-08-23
卷: 296, 期:3, 页:657-663
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: ADRENAL CHROMAFFIN CELLS ; PROTEIN-KINASE ; MOLECULAR-CLONING ; 14-3-3-PROTEINS ; ISCHEMIA ; EXPRESSION ; SIGNAL ; RAF-1
英文摘要:

The 14-3-3 protein family plays critical regulatory roles in signaling pathways in cell division and apoptosis. 14-3-3gamma is mainly expressed in brain. Using primary cultures of cerebral cortical astrocytes, we investigated the relationships between 14-3-3gamma proteins and actin in astrocytes in cell division and under ischemia. Our results showed that endogenous 14-3-3gamma proteins in immature astrocytes appeared filamentous and co-localized with filamentous actin (F-actin). During certain stages of mitosis, 14-3-3gamma proteins first aggregated and then formed a ring-like structure that surrounded the daughter nuclei and enclosed the F-actin. In 4-week-old cultures of astrocytes, 14-3-3gamma proteins appeared as punctate aggregates in the cytoplasm. Under ischemia, 14-3-3gamma proteins formed filamentous structures and were closely associated with F-actin in surviving astrocytes. However, in apoptotic astrocytes, the intensity of immunostaining of 14-3-3gamma proteins in the cytoplasm decreased. The proteins aggregated around the nucleus and dissociated from the actin filaments. Reciprocal co-immunoprecipitations demonstrated that endogenous 14-3-3gamma proteins bound to detergent-soluble actin and the level of binding increased after 4 h of ischemia. As actin is a critical structural protein heavily involved in cell division and apoptotic death, our findings suggest that 14-3-3gamma proteins play a role in cytoskeletal function during the process of cell division and apoptosis in astrocytes in association with actin. (C) 2002 Elsevier Science (USA). All rights reserved.

语种: 英语
WOS记录号: WOS:000177747800023
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67757
Appears in Collections:基础医学院_神经生物学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes.pdf(372KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100083, Peoples R China
3.Hong Kong Univ Sci & Technol, Dept Biol, Hong Kong, Hong Kong, Peoples R China
4.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Chen, XQ,Yu, ACH. The association of 14-3-3 gamma and actin plays a role in cell division and apoptosis in astrocytes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2002,296(3):657-663.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Chen, XQ]'s Articles
[Yu, ACH]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Chen, XQ]‘s Articles
[Yu, ACH]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace