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学科主题: 临床医学
题名:
A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels
作者: Guo Xue-qian1; Tian Bei2,3; Liu Zhi-cheng1; Wei Wen-bin2,3; Tao Yong4; Sun Shi-jie1; Zhang Yu1
关键词: glaucoma ; rat ; retina ; silicone oil ; electrocoagulation ; corneal limbus
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2011-01-20
DOI: 10.3760/cma.j.issn.0366-6999.2011.02.029
卷: 124, 期:2, 页:309-314
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: ELEVATED INTRAOCULAR-PRESSURE ; GANGLION-CELL DEATH ; LASER PHOTOCOAGULATION ; TRABECULAR MESHWORK ; RABBIT ; DAMAGE ; SIZE
英文摘要:

Background A satisfied glaucoma model is absent now. The aim of this study was to evaluate the effect of a combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins in the rats to establish glaucoma model.

Methods Operation was performed in each of the left eyes of 90 adult male rats. Right eyes were used as controls. Measurement of intraocular pressure (IOP) was performed with an applanation tonometer (Tono-Pen). Retinal ganglion cells (RGCs) were retrogradely labeled by applying FluoroGold onto the bilateral superior colliculus.

Results During the follow-up (24 weeks), the IOP of the study eyes was significantly higher (P <0.05) than the control eyes (at final examination, IOP of control eyes was (13.4 +/- 1.0) mmHg and IOP of study eyes was (16.1 +/- 1.8) mmHg). Correspondingly, at 24 weeks after operation, the RGCs density of the study eyes (2286.11 +/- 290.45/mm(2)) was significantly lower than the control eyes (2626.46 +/- 164.85/mm(2), P <0.01). In the operated eyes, histological examination showed excavation of optic disc and increased neuroglial cells in the optic nerve, reduced thickness of retina and diminution of retinal ganglion cells, and atrophy of ciliary body and iris. Notably, the anterior chamber angle of the operated eye remained open.

Conclusions A combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins may establish a reliable glaucoma model for further research. Chin Med J 2011;124(2):309-314

语种: 英语
所属项目编号: 30670514 ; KM200910025007 ; 2009B04
项目资助者: National Natural Science Foundation of China ; Program of Beijing Education Commission ; Beijing Novel Program
WOS记录号: WOS:000286787300031
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67768
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Beijing Ophthalmol & Visual Sci Key Lab, Beijing 100730, Peoples R China
2.Capital Med Univ, Sch Biomed Engn, Beijing 100069, Peoples R China
3.Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China
4.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China

Recommended Citation:
Guo Xue-qian,Tian Bei,Liu Zhi-cheng,et al. A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels[J]. CHINESE MEDICAL JOURNAL,2011,124(2):309-314.
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