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A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels
Guo Xue-qian1; Tian Bei2,3; Liu Zhi-cheng1; Wei Wen-bin2,3; Tao Yong4; Sun Shi-jie1; Zhang Yu1
关键词glaucoma rat retina silicone oil electrocoagulation corneal limbus
刊名CHINESE MEDICAL JOURNAL
2011-01-20
DOI10.3760/cma.j.issn.0366-6999.2011.02.029
124期:2页:309-314
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
资助者National Natural Science Foundation of China ; Program of Beijing Education Commission ; Beijing Novel Program ; National Natural Science Foundation of China ; Program of Beijing Education Commission ; Beijing Novel Program
研究领域[WOS]General & Internal Medicine
关键词[WOS]ELEVATED INTRAOCULAR-PRESSURE ; GANGLION-CELL DEATH ; LASER PHOTOCOAGULATION ; TRABECULAR MESHWORK ; RABBIT ; DAMAGE ; SIZE
英文摘要

Background A satisfied glaucoma model is absent now. The aim of this study was to evaluate the effect of a combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins in the rats to establish glaucoma model.

Methods Operation was performed in each of the left eyes of 90 adult male rats. Right eyes were used as controls. Measurement of intraocular pressure (IOP) was performed with an applanation tonometer (Tono-Pen). Retinal ganglion cells (RGCs) were retrogradely labeled by applying FluoroGold onto the bilateral superior colliculus.

Results During the follow-up (24 weeks), the IOP of the study eyes was significantly higher (P <0.05) than the control eyes (at final examination, IOP of control eyes was (13.4 +/- 1.0) mmHg and IOP of study eyes was (16.1 +/- 1.8) mmHg). Correspondingly, at 24 weeks after operation, the RGCs density of the study eyes (2286.11 +/- 290.45/mm(2)) was significantly lower than the control eyes (2626.46 +/- 164.85/mm(2), P <0.01). In the operated eyes, histological examination showed excavation of optic disc and increased neuroglial cells in the optic nerve, reduced thickness of retina and diminution of retinal ganglion cells, and atrophy of ciliary body and iris. Notably, the anterior chamber angle of the operated eye remained open.

Conclusions A combination of intracameral injection of silicone oil and electrocoagulation of corneal limbal vessels and episcleral veins may establish a reliable glaucoma model for further research. Chin Med J 2011;124(2):309-314

语种英语
所属项目编号30670514 ; KM200910025007 ; 2009B04
资助者National Natural Science Foundation of China ; Program of Beijing Education Commission ; Beijing Novel Program ; National Natural Science Foundation of China ; Program of Beijing Education Commission ; Beijing Novel Program
WOS记录号WOS:000286787300031
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67768
Collection北京大学第二临床医学院_眼科
作者单位1.Beijing Ophthalmol & Visual Sci Key Lab, Beijing 100730, Peoples R China
2.Capital Med Univ, Sch Biomed Engn, Beijing 100069, Peoples R China
3.Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Beijing 100730, Peoples R China
4.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Guo Xue-qian,Tian Bei,Liu Zhi-cheng,et al. A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels[J]. CHINESE MEDICAL JOURNAL,2011,124(2):309-314.
APA Guo Xue-qian.,Tian Bei.,Liu Zhi-cheng.,Wei Wen-bin.,Tao Yong.,...&Zhang Yu.(2011).A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels.CHINESE MEDICAL JOURNAL,124(2),309-314.
MLA Guo Xue-qian,et al."A new rat model of glaucoma induced by intracameral injection of silicone oil and electrocoagulation of limbal vessels".CHINESE MEDICAL JOURNAL 124.2(2011):309-314.
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