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学科主题: 口腔医学
题名:
Gene Expression Dynamics during Diabetic Periodontitis
作者: Andriankaja, O. M.1,2; Galicia, J.1; Dong, G.1; Xiao, W.1,3; Alawi, F.4; Graves, D. T.1
关键词: DAVID ; diabetes ; GSEA ; inflammation ; microarray ; periodontal disease(s)
刊名: JOURNAL OF DENTAL RESEARCH
发表日期: 2012-12-01
DOI: 10.1177/0022034512465292
卷: 91, 期:12, 页:1160-1165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: ENHANCED INFLAMMATION ; BONE-FORMATION ; APOPTOSIS ; GINGIVITIS ; RESOLUTION ; DISEASES ; RECEPTOR ; REPAIR ; RATS
英文摘要:

Diabetes impairs the resolution of periodontal inflammation. We explored pathways altered by inflammation in the diabetic periodontium by using ligatures to induce periodontitis in type-2 diabetic Goto-Kakizaki rats. Ligatures were removed after 7 days, and rats were then treated with TNF inhibitor (pegsunercept) or vehicle alone and euthanized 4 days later. RNA was extracted from periodontal tissue, examined by mRNA profiling, and further analyzed by functional criteria. We found that 1,754 genes were significantly up-regulated and 1,243 were down-regulated by pegsunercept (p < 0.05). Functional analysis revealed up-regulation of neuron-associated and retina-associated gene clusters as well as those related to cell activity and signaling. Others were down-regulated by TNF inhibition and included genes associated with host defense, apoptosis, cell signaling and activity, and coagulation/hemostasis/complement. For selected genes, findings with microarray and rt-PCR agreed. PPAR-alpha was investigated further by immunohistochemistry due to its anti-inflammatory function and was found to be up-regulated in the gingiva during the resolution of periodontal inflammation and suppressed by diabetes. The results indicate that diabetes-enhanced inflammation both up- and down-regulates genes involved in cellular activity and cell signaling, while it predominantly up-regulates genes involved in the host response, apoptosis, and coagulation/homeostasis/complement and down-regulates mRNA levels of neuron, retina, and energy/metabolism-associated genes.

语种: 英语
所属项目编号: DE017732
项目资助者: NIDCR
WOS记录号: WOS:000311037600012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67786
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Univ Penn, Sch Dent Med, Dept Periodont, Philadelphia, PA 19104 USA
2.Univ Puerto Rico, Sch Dent Med, Ctr Clin Res & Hlth Promot, San Juan, PR 00936 USA
3.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing, Peoples R China
4.Univ Penn, Sch Dent Med, Dept Pathol, Philadelphia, PA 19104 USA

Recommended Citation:
Andriankaja, O. M.,Galicia, J.,Dong, G.,et al. Gene Expression Dynamics during Diabetic Periodontitis[J]. JOURNAL OF DENTAL RESEARCH,2012,91(12):1160-1165.
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