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学科主题口腔医学
Gene Expression Dynamics during Diabetic Periodontitis
Andriankaja, O. M.1,2; Galicia, J.1; Dong, G.1; Xiao, W.1,3; Alawi, F.4; Graves, D. T.1
关键词DAVID diabetes GSEA inflammation microarray periodontal disease(s)
刊名JOURNAL OF DENTAL RESEARCH
2012-12-01
DOI10.1177/0022034512465292
91期:12页:1160-1165
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]ENHANCED INFLAMMATION ; BONE-FORMATION ; APOPTOSIS ; GINGIVITIS ; RESOLUTION ; DISEASES ; RECEPTOR ; REPAIR ; RATS
英文摘要

Diabetes impairs the resolution of periodontal inflammation. We explored pathways altered by inflammation in the diabetic periodontium by using ligatures to induce periodontitis in type-2 diabetic Goto-Kakizaki rats. Ligatures were removed after 7 days, and rats were then treated with TNF inhibitor (pegsunercept) or vehicle alone and euthanized 4 days later. RNA was extracted from periodontal tissue, examined by mRNA profiling, and further analyzed by functional criteria. We found that 1,754 genes were significantly up-regulated and 1,243 were down-regulated by pegsunercept (p < 0.05). Functional analysis revealed up-regulation of neuron-associated and retina-associated gene clusters as well as those related to cell activity and signaling. Others were down-regulated by TNF inhibition and included genes associated with host defense, apoptosis, cell signaling and activity, and coagulation/hemostasis/complement. For selected genes, findings with microarray and rt-PCR agreed. PPAR-alpha was investigated further by immunohistochemistry due to its anti-inflammatory function and was found to be up-regulated in the gingiva during the resolution of periodontal inflammation and suppressed by diabetes. The results indicate that diabetes-enhanced inflammation both up- and down-regulates genes involved in cellular activity and cell signaling, while it predominantly up-regulates genes involved in the host response, apoptosis, and coagulation/homeostasis/complement and down-regulates mRNA levels of neuron, retina, and energy/metabolism-associated genes.

语种英语
WOS记录号WOS:000311037600012
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被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67786
专题北京大学口腔医学院_牙周科
作者单位1.Univ Penn, Sch Dent Med, Dept Periodont, Philadelphia, PA 19104 USA
2.Univ Puerto Rico, Sch Dent Med, Ctr Clin Res & Hlth Promot, San Juan, PR 00936 USA
3.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing, Peoples R China
4.Univ Penn, Sch Dent Med, Dept Pathol, Philadelphia, PA 19104 USA
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GB/T 7714
Andriankaja, O. M.,Galicia, J.,Dong, G.,et al. Gene Expression Dynamics during Diabetic Periodontitis[J]. JOURNAL OF DENTAL RESEARCH,2012,91(12):1160-1165.
APA Andriankaja, O. M.,Galicia, J.,Dong, G.,Xiao, W.,Alawi, F.,&Graves, D. T..(2012).Gene Expression Dynamics during Diabetic Periodontitis.JOURNAL OF DENTAL RESEARCH,91(12),1160-1165.
MLA Andriankaja, O. M.,et al."Gene Expression Dynamics during Diabetic Periodontitis".JOURNAL OF DENTAL RESEARCH 91.12(2012):1160-1165.
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