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学科主题: 临床医学
题名:
Validation study of I-131-RRL: Assessment of biodistribution, SPECT imaging and radiation dosimetry in mice
作者: Zhao, Qian; Yan, Ping; Yin, Lei; Li, Ling; Chen, Xue Qi; Ma, Chao; Wang, Rong Fu
关键词: I-131 ; peptide ; tumor angiogenesis ; molecular imaging ; biodistribuiton ; radiation dosimetry
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2013-04-01
DOI: 10.3892/mmr.2013.1338
卷: 7, 期:4, 页:1355-1360
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: ARGININE-ARGININE-LEUCINE ; HUMAN PROSTATE CARCINOMA ; INTERNAL DOSE ASSESSMENT ; PEPTIDE ; TUMORS ; ANGIOGENESIS ; CANCER
英文摘要:

Tumor angiogenesis is important in the growth and metastasis of malignant tumors. In our previous study, we demonstrated that an arginine-arginine-leucine (RRL) peptide is a tumor endothelial cell-specific binding sequence that may be used as a molecular probe for the imaging of malignant tumors in vivo. The aim of the present study was to further explore the characteristics of I-131-RRL by biodistribution tests, and to estimate the radiation dosimetry of I-131-RRL for humans using mice data. The RRL peptide was radiolabeled with I-131 by a chloramine-T (CH-T) method. The radiolabeling efficiency and radiochemical purity were then characterized in vitro. I-131-RRL was injected intravenously into B16 xenograft-bearing Kunming mice. Biodistribution analysis and in vivo imaging were performed periodically. The radiation dosimetry in humans was calculated according to the organ distribution and the standard medical internal radiation dose (MIRD) method in mice. All data were analyzed by statistical and MIRDOSE 3.1 software. The labeling efficiency of I-131-RRL reached 70.0 +/- 2.91% (n=5), and the radiochemical purity exceeded 95% following purification. In mice bearing B16 xenografts, I-131-RRL rapidly cleared from the blood and predominantly accumulated in the kidneys, the stomach and the tumor tissue. The specific uptake of I-131-RRL in the tumor increased over time and was significantly higher than that of the other organs, 24-72 h following injection (P<0.05). The ratio of tumor-to-skeletal muscle (T/SM) tissue exceeded 4.75, and the ratio of the tumor-to-blood (T/B) tissue peaked at 3.36. In the single-photon emission computed tomography (SPECT) imaging of Kunming mice bearing B16 xenografts, the tumors were clearly identifiable at 6 h, and significant uptake was evident 24-72 h following administration of I-131-RRL. The effective dose for the adult male dosimetric model was estimated to be 0.0293 mSv/MBq. Higher absorbed doses were estimated for the stomach (0.102 mGy/MBq), the small intestines (0.0699 mGy/MBq), the kidneys (0.0611 mGy/MBq) and the liver (0.055 mGy/MBq). These results highlight the potential of I-131-RRL as a ligand for the SPECT imaging of tumors. Administration of I-131-RRL led to a reasonable radiation dose burden and was safe for human use.

语种: 英语
所属项目编号: 30870729 ; 81071183 ; 2011YQ030114 ; 2011YQ03011409 ; BMU20120297 ; 110202 ; 120201
项目资助者: Natural Science Foundation of China (NSFC) ; Ministry of Science and Technology of China ; Research Fund for the Medicine and Engineering of Peking University (Beijing, China) ; Research Fund of the Key Laboratory of Radiopharmaceuticals, Beijing Normal University, China ; Ministry of Education, Department of Chemistry, Beijing Normal University
WOS记录号: WOS:000321467100049
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67791
Appears in Collections:北京大学第一临床医学院_核医学科_期刊论文

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作者单位: Peking Univ First Hosp, Dept Nucl Med, Beijing 100034, Peoples R China

Recommended Citation:
Zhao, Qian,Yan, Ping,Yin, Lei,et al. Validation study of I-131-RRL: Assessment of biodistribution, SPECT imaging and radiation dosimetry in mice[J]. MOLECULAR MEDICINE REPORTS,2013,7(4):1355-1360.
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