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学科主题基础医学
Angiotensin II up-regulates soluble epoxide hydrolase in vascular endothelium in vitro and in vivo
Ai, Ding; Fu, Yi; Guo, Deliang; Tanaka, Hiromasa; Wang, Nanping; Tang, Chaoshu; Hammock, Bruce D.; Shyy, John Y. -J.; Zhu, Yi
关键词endothelial cells arachidonic acid AP-1 promoter hypertension
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
2007-05-22
DOI10.1073/pnas.0703229104
104期:21页:9018-9023
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]EPOXYGENASE-DERIVED EICOSANOIDS ; SMOOTH-MUSCLE-CELLS ; KAPPA-B KINASE ; EPOXYEICOSATRIENOIC ACIDS ; ACTIVATOR PROTEIN-1 ; GENE-EXPRESSION ; HYPERTENSION ; PHOSPHORYLATION ; PATHWAYS ; P65
英文摘要

Epoxyeicosatrienoic acids (EETs), as metabolites of arachidonic acid, may function as antihypertensive and antiatherosclerotic mediators for vasculature. EETs are degraded by soluble epoxide hydrolase (sEH). Pharmacological inhibition and genetic ablation of sEH have been shown to increase the level of EETs, and treating angiotensin II (Ang II)-infused hypertension rats with sEH-selective inhibitors increased the levels of EETs with attendant decrease in systolic blood pressure. To elucidate the mechanisms by which Ang II regulates sEH expression, we treated human umbilical vein endothelial cells (ECs) and bovine aortic ECs with Ang II and found increased sEH expression at both the mRNA and protein levels. Transient transfection assays showed that the activity of the human sEH promoter was increased in ECs in response to Ang II. Further analysis of the promoter region of the sEH gene demonstrated that treatment with Ang II, like overexpression of c-Jun/c-Fos, activates the sEH promoter through an AP-1-binding motif. The binding of c-Jun to the AP-1 site of the sEH promoter was confirmed by chromatin immunoprecipitation assays. in contrast, adenovirus overexpression of the dominant-negative mutant of c-Jun significantly attenuated the effects of Ang II on sEH induction. An elevated level of sEH was found in the aortic intima of both spontaneously hypertensive rats and Ang II-infused Wistar rats. Blocking Ang II binding to Ang II receptor 1 by losartan abolished the sEH induction. Thus, AP-1 activation is involved in the transcriptional up-regulation of sEH by Ang II in ECs, which may contribute to Ang II-induced hypertension.

语种英语
WOS记录号WOS:000246853700061
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被引频次:109[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67797
专题北京大学基础医学院_心血管所
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Educ Minist, Beijing 100083, Peoples R China
2.Peking Univ, Cardiovasc Res Inst, Beijing 100083, Peoples R China
3.Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA
4.Univ Calif Davis, Dept Entomol, Davis, CA 95616 USA
5.Univ Calif Davis, Canc Res Ctr, Davis, CA 95616 USA
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GB/T 7714
Ai, Ding,Fu, Yi,Guo, Deliang,et al. Angiotensin II up-regulates soluble epoxide hydrolase in vascular endothelium in vitro and in vivo[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2007,104(21):9018-9023.
APA Ai, Ding.,Fu, Yi.,Guo, Deliang.,Tanaka, Hiromasa.,Wang, Nanping.,...&Zhu, Yi.(2007).Angiotensin II up-regulates soluble epoxide hydrolase in vascular endothelium in vitro and in vivo.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,104(21),9018-9023.
MLA Ai, Ding,et al."Angiotensin II up-regulates soluble epoxide hydrolase in vascular endothelium in vitro and in vivo".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104.21(2007):9018-9023.
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