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IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科  > 期刊论文
学科主题: 临床医学
题名:
De novo sequencing of antigen-derived peptides expressed on the surface of human liver cancer HLE cells by nanoelectrospray tandem mass spectrometry
作者: Wang Hongxia; Li Ping; He Jiatian; Zhou Mai; Liu Bingyu; Yang Songcheng; Zhang Xuemin
关键词: HLE cells ; antigen-derived peptides at the cell surface ; electrospray tandem mass spectrometry ; de novo sequencing
刊名: CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
发表日期: 2006-07-01
卷: 34, 期:7, 页:915-918
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: IDENTIFICATION ; PROTEINS ; BRAIN
英文摘要:

Antigen-derived peptides expressed at the cell surface of tumor cells may be recognized by melanoma-specific cytotoxic T lymphocytes (CTLs) and could be used in peptide-based melanoma vaccines. Such peptides from human liver cancer HLE cells surface were eluted by weak acid and were subsequently fractionated by reverse phase high performance liquid chromatography (RP-HPLC). The peptides from human liver cells were also eluted and then separated by RP-HPLC with the exact same methods. Five peaks specifically existed in FILE cells after comparing the RP-HPLC spectrum with the normal liver cells. Nanoelectrospray tandem mass spectrometry (nano ESI-MS/MS) was used to sequence and identify these peptide. Twenty peptides were sequenced and among these 12 got confirmed amino acid sequence after sequence analysis. The mass range of these peptides were 1000 - 2000 Da. After database search, three peptides were derived from protein calcium calmodulin, ribosomal protein S19 and chaperonin 10 respectively and the other nine peptides were new peptides. The biological function of these peptides and their relationship with tumor need be studied further. This study showed that nanoESI-MS/MS is a powerful tool for peptide sequencing from mixture with small amount.

语种: 中文
WOS记录号: WOS:000242858300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67800
Appears in Collections:北京大学第二临床医学院_肝胆外科_期刊论文

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作者单位: 1.Natl Ctr Biomed Anal, Beijing 100850, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China

Recommended Citation:
Wang Hongxia,Li Ping,He Jiatian,et al. De novo sequencing of antigen-derived peptides expressed on the surface of human liver cancer HLE cells by nanoelectrospray tandem mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(7):915-918.
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