IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科
学科主题临床医学
De novo sequencing of antigen-derived peptides expressed on the surface of human liver cancer HLE cells by nanoelectrospray tandem mass spectrometry
Wang Hongxia; Li Ping; He Jiatian; Zhou Mai; Liu Bingyu; Yang Songcheng; Zhang Xuemin
关键词HLE cells antigen-derived peptides at the cell surface electrospray tandem mass spectrometry de novo sequencing
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2006-07-01
34期:7页:915-918
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]IDENTIFICATION ; PROTEINS ; BRAIN
英文摘要

Antigen-derived peptides expressed at the cell surface of tumor cells may be recognized by melanoma-specific cytotoxic T lymphocytes (CTLs) and could be used in peptide-based melanoma vaccines. Such peptides from human liver cancer HLE cells surface were eluted by weak acid and were subsequently fractionated by reverse phase high performance liquid chromatography (RP-HPLC). The peptides from human liver cells were also eluted and then separated by RP-HPLC with the exact same methods. Five peaks specifically existed in FILE cells after comparing the RP-HPLC spectrum with the normal liver cells. Nanoelectrospray tandem mass spectrometry (nano ESI-MS/MS) was used to sequence and identify these peptide. Twenty peptides were sequenced and among these 12 got confirmed amino acid sequence after sequence analysis. The mass range of these peptides were 1000 - 2000 Da. After database search, three peptides were derived from protein calcium calmodulin, ribosomal protein S19 and chaperonin 10 respectively and the other nine peptides were new peptides. The biological function of these peptides and their relationship with tumor need be studied further. This study showed that nanoESI-MS/MS is a powerful tool for peptide sequencing from mixture with small amount.

语种中文
WOS记录号WOS:000242858300004
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67800
Collection北京大学第二临床医学院_肝胆外科
作者单位1.Natl Ctr Biomed Anal, Beijing 100850, Peoples R China
2.Peking Univ, Peoples Hosp, Ctr Hepatobiliary Surg, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Wang Hongxia,Li Ping,He Jiatian,et al. De novo sequencing of antigen-derived peptides expressed on the surface of human liver cancer HLE cells by nanoelectrospray tandem mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(7):915-918.
APA Wang Hongxia.,Li Ping.,He Jiatian.,Zhou Mai.,Liu Bingyu.,...&Zhang Xuemin.(2006).De novo sequencing of antigen-derived peptides expressed on the surface of human liver cancer HLE cells by nanoelectrospray tandem mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(7),915-918.
MLA Wang Hongxia,et al."De novo sequencing of antigen-derived peptides expressed on the surface of human liver cancer HLE cells by nanoelectrospray tandem mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.7(2006):915-918.
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