IR@PKUHSC  > 北京大学第一临床医学院  > 感染疾病科
学科主题临床医学
Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells
Liu, MC; Yu, M; Zhang, NL; Gong, WB; Wang, Y; Piao, WH; Wang, QH; Wang, GQ
关键词correlation covalently closed circular DNA hepatitis B virus antigen hepatitis B virus DNA
刊名JOURNAL OF VIRAL HEPATITIS
2004-03-01
11期:2页:124-129
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology ; Infectious Diseases ; Virology
研究领域[WOS]Gastroenterology & Hepatology ; Infectious Diseases ; Virology
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; EXPERIMENTAL-INFECTION ; HBV REPLICATION ; HEPG2 CELLS ; VIRAL-DNA ; IN-VITRO ; DUCK
英文摘要

The 2.2.15 cells-derived from HepG2 cells transfected with a plasmid containing hepatitis B virus (HBV) DNA secrete surface antigen (HBsAg) particles, nucleocapsids and virions (Proc Natl Acad Sci U S A 1987; 84: 1005-1009). The latter elicit acute hepatitis in chimpanzees (Proc Natl Acad Sci U S A 1987; 84: 4641-4644). We studied the presence of intracellular and extracellular HBV covalently closed circular (ccc) DNA in this culture system by polymerase chain reaction (PCR), kinetically analysed HBsAg and hepatitis B e antigen (HBeAg) released in the culture media by quantitative enzyme-linked immunosorbent assay and quantitated by real-time PCR but HBV DNA from intracellular and extracellular HBV-DNA. HBV cccDNA was found both intracellularly and extracellularly. A significant correlation was seen between the extracellular HBV DNA levels and virus antigens (r = 0.833; P = 0.01 and r = 0.939; P < 0.01 for HBsAg and HBeAg, respectively), whereas there was no statistical correlation between intracellular HBV DNA levels and virus antigen levels (r = 0.024; P = 0.955 and r = 0.177; P = 0.625 for HBsAg and HBeAg, respectively). These data would be valuable in studies of the HBV life cycle and of potential anti-viral agents.

语种英语
WOS记录号WOS:000189141900004
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67802
专题北京大学第一临床医学院_感染疾病科
作者单位Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Liu, MC,Yu, M,Zhang, NL,et al. Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells[J]. JOURNAL OF VIRAL HEPATITIS,2004,11(2):124-129.
APA Liu, MC.,Yu, M.,Zhang, NL.,Gong, WB.,Wang, Y.,...&Wang, GQ.(2004).Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells.JOURNAL OF VIRAL HEPATITIS,11(2),124-129.
MLA Liu, MC,et al."Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells".JOURNAL OF VIRAL HEPATITIS 11.2(2004):124-129.
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