北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 感染疾病科  > 期刊论文
学科主题: 临床医学
题名:
Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells
作者: Liu, MC; Yu, M; Zhang, NL; Gong, WB; Wang, Y; Piao, WH; Wang, QH; Wang, GQ
关键词: correlation ; covalently closed circular DNA ; hepatitis B virus antigen ; hepatitis B virus DNA
刊名: JOURNAL OF VIRAL HEPATITIS
发表日期: 2004-03-01
卷: 11, 期:2, 页:124-129
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Infectious Diseases ; Virology
研究领域[WOS]: Gastroenterology & Hepatology ; Infectious Diseases ; Virology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; EXPERIMENTAL-INFECTION ; HBV REPLICATION ; HEPG2 CELLS ; VIRAL-DNA ; IN-VITRO ; DUCK
英文摘要:

The 2.2.15 cells-derived from HepG2 cells transfected with a plasmid containing hepatitis B virus (HBV) DNA secrete surface antigen (HBsAg) particles, nucleocapsids and virions (Proc Natl Acad Sci U S A 1987; 84: 1005-1009). The latter elicit acute hepatitis in chimpanzees (Proc Natl Acad Sci U S A 1987; 84: 4641-4644). We studied the presence of intracellular and extracellular HBV covalently closed circular (ccc) DNA in this culture system by polymerase chain reaction (PCR), kinetically analysed HBsAg and hepatitis B e antigen (HBeAg) released in the culture media by quantitative enzyme-linked immunosorbent assay and quantitated by real-time PCR but HBV DNA from intracellular and extracellular HBV-DNA. HBV cccDNA was found both intracellularly and extracellularly. A significant correlation was seen between the extracellular HBV DNA levels and virus antigens (r = 0.833; P = 0.01 and r = 0.939; P < 0.01 for HBsAg and HBeAg, respectively), whereas there was no statistical correlation between intracellular HBV DNA levels and virus antigen levels (r = 0.024; P = 0.955 and r = 0.177; P = 0.625 for HBsAg and HBeAg, respectively). These data would be valuable in studies of the HBV life cycle and of potential anti-viral agents.

语种: 英语
WOS记录号: WOS:000189141900004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67802
Appears in Collections:北京大学第一临床医学院_感染疾病科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Hosp 1, Dept Infect Dis, Beijing 100034, Peoples R China

Recommended Citation:
Liu, MC,Yu, M,Zhang, NL,et al. Dynamic analysis of hepatitis B virus DNA and its antigens in 2.2.15 cells[J]. JOURNAL OF VIRAL HEPATITIS,2004,11(2):124-129.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, MC]'s Articles
[Yu, M]'s Articles
[Zhang, NL]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, MC]‘s Articles
[Yu, M]‘s Articles
[Zhang, NL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace