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学科主题: 口腔医学
题名:
Electrospun Gelatin/beta-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling
作者: Zhang, Xuehui1,2; Meng, Song1,3; Huang, Ying1; Xu, Mingming1; He, Ying1; Lin, Hong2; Han, Jianmin2; Chai, Yuan2; Wei, Yan1; Deng, Xuliang1,4,5
刊名: STEM CELLS INTERNATIONAL
发表日期: 2015
DOI: 10.1155/2015/507154
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell & Tissue Engineering
研究领域[WOS]: Cell Biology
关键词[WOS]: MESENCHYMAL STEM-CELLS ; BETA-TRICALCIUM PHOSPHATE ; BIOMIMETIC NANOCOMPOSITES ; STROMAL CELLS ; SCAFFOLDS ; CALCIUM ; REGENERATION ; HYDROXYAPATITE ; RELEASE ; MINERALIZATION
英文摘要:

Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/beta-tricalcium phosphate (TCP) and confirmed their biological activity in vitro and bone regeneration in vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/beta-TCP composite nanofibers were fabricated by incorporating 20 wt% beta-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the composite beta-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of the beta-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/beta-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/beta-TCP composite scaffolds promote osteogenic differentiation of BMSCs in vitro and bone regeneration in vivo by activating Ca2+-sensing receptor signaling.

语种: 英语
所属项目编号: 2012CB933900 ; 2011DFA32190 ; 81171000 ; 20130001120112 ; 2015AA032004 ; 2012AA022501 ; Z14111000180000
项目资助者: National Basic Research Program of China ; Key International S&amp ; T Cooperation Project ; National Natural Science Foundation of China ; Doctoral Scientific Fund Project of the Ministry of Education of China ; National High Technology Research and Development Program of China ; Beijing Nova Program
WOS记录号: WOS:000356385900001
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/67813
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Geriatr Dent, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Dent Mat, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Dept Prosthodont, Beijing 100081, Peoples R China
4.Natl Engn Lab Digital & Mat Technol Stomatol, Beijing 100081, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Beijing Lab Biomed Mat, Beijing 100081, Peoples R China

Recommended Citation:
Zhang, Xuehui,Meng, Song,Huang, Ying,et al. Electrospun Gelatin/beta-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling[J]. STEM CELLS INTERNATIONAL,2015.
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