IR@PKUHSC  > 北京大学第一临床医学院  > 临床药理研究所
学科主题临床医学
Determination of Antofloxacin in Human Plasma by Solid Phase Extraction-Liquid Chromatography-Mass Spectrometry
Wei Min-Ji; Zhao Cai-Yun; Mao Long-Qing; Kang Zi-Sheng; Li Tian-Yun; Lu Yuan
关键词High performance liquid chromatography-tandem mass spectrometry Solid phase extraction Antofloxacin
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2012-05-01
DOI10.3724/SP.J.1096.2012.10460
40期:5页:787-790
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]HYDROCHLORIDE ; FLUOROQUINOLONE
英文摘要

A new and specific method has been developed for the determination of antofloxacin in human plasma by solid phase extraction-liquid chromatography coupled with tandem spectrometry. The samples were extracted and purified by wean anion solid phase extraction. The eluent was dried and reconstituted before analysis. The separation was performed on an Agilent Eclipse plus C-8 column, with a mobile phase consisting of 10 mmol/L ammonium acetate(pH 3.5)-methanol(35:65, V/V) at a flow of 0.35 mL/min. Identification and quantification were achieved by LC-MS/MS in a positive mode and multiple reactions monitoring (MRM). Good linearity was observed in a range of 7.0-5080 mu g/L with a correlation coefficient of more than 0.996. The detection limit was 0.5 mu g/L. Sample recovery was >77.6%, and inter-day coefficient of variation was less than 7.9%. The proposed method has been applied to the determination of antofloxacin in plasma after subjects were orally given 400 mg of antofloxacin tablets.

语种中文
WOS记录号WOS:000313814700018
Citation statistics
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/67814
Collection北京大学第一临床医学院_临床药理研究所
作者单位Peking Univ, Hosp 1, Inst Clin Pharmacol, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Wei Min-Ji,Zhao Cai-Yun,Mao Long-Qing,et al. Determination of Antofloxacin in Human Plasma by Solid Phase Extraction-Liquid Chromatography-Mass Spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2012,40(5):787-790.
APA Wei Min-Ji,Zhao Cai-Yun,Mao Long-Qing,Kang Zi-Sheng,Li Tian-Yun,&Lu Yuan.(2012).Determination of Antofloxacin in Human Plasma by Solid Phase Extraction-Liquid Chromatography-Mass Spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,40(5),787-790.
MLA Wei Min-Ji,et al."Determination of Antofloxacin in Human Plasma by Solid Phase Extraction-Liquid Chromatography-Mass Spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 40.5(2012):787-790.
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